Nature of DNA-bound fatty acids inPseudomonas aurantiaca

Abstract

The existence of Pseudomonas aurantiaca DNA-bound fatty acids and lipids is presented in this work. The isolation of DNA was carried out by two different procedures, namely, phenol and detergent-based phenol isolation in order to prove the presence of DNA-bound lipids. The lipid content of DNA is expressed in terms of fatty acid profile. A high level of 16:0, 18:0 and 18:1 is characteristic for tightly bound DNA lipids. On the other hand, the fatty acids such as 14:1, iso14:0 and iso16:0 are found in trace amounts only in DNA lipid fraction, but these fatty acids are not found in the whole-cell lipids. Absolutely no 3-hydroxy fatty acids were found in DNA lipids. However, both C16 and C18 species represent the main fatty acids of whole-cell and DNA-bound lipids. The presence of DNA-bound lipids even under tough treatment of DNA allows to conclude that these lipids represent a special pool among cellular lipids.

Biomarkers of Bacillus subtilis total lipids FAME profile under various temperatures and growth phases

“…Some researchers studied the influence of environmental factors on the growth of bacterial strains by changes in their FAME profiles [4][5][6]. In some cases, specific markers of the influence of environmental factors on bacterial strains were found, which are commonly used to identify strains under different conditions [2,7]. The Bacillus subtilis strain OSU 142, which was used in this work, is a nitrogen fixing Gram positive bacterium exhibiting activity against a broad spectrum of pathogens (bacte ria and fungi).…”