DNA Carbon-Nanodots based Electrochemical Biosensor for Detection of Mutagenic Nitrosamines

Abstract

Mutagenic and Carcinogenic substances are a threat to any living organism, and its detection is of paramount importance. In this work, we fabricate for the first time a DNA-carbon dots based electrochemical biosensor for sensitive and selective detection of mutagenic nitrosamines like N-nitrosodimethylamine (NDMA) and N-nitrosodiethanolamine (NDEA). At first, on the glassy carbon electrode (GCE), chitosan carbon dot was deposited, then, DNA was electro-statically immobilizing on the surface of carbon dots to fabricate the sensing electrode (DNA/chiCD/GCE modified electrode). In the presence of NDMA and NDEA, in differential pulse voltammetry technique, the absolute peak current increases, and thus it can detect NDMA and NDEA. The system DNA/chiCD/GCE modified electrode is highly selective and sensitive toward NDMA and NDEA. The detection limit was determined to be 9.9 × 10–9 M and 9.6 × 10–9 M, respectively. The possible reason for DNA/chiCD/GCE modified electrode showing such electrochemical selectivity toward nitrosamines is investigated and discussed.

Electroanalytical overview: utilising micro- and nano-dimensional sized materials in electrochemical-based biosensing platforms

“…Carbon quantum dots have been used in conjunction with DNA as a biorecognition element through drop-casting [51] due to their high surface area and multiple feasible binding sites. Majumdar et al [50] reported this using carbon quantum dots from chitosan modified with DNA for the detection of mutagenic nitrosamines N-nitrosodimethylamine (NDMA) and N-nitrosodiethanolamine (NDEA). This is achieved through the drop-casting of the chitosan carbon dots onto a GCE followed by drop-casting of DNA (Fig.…”

Application of DNA-Nanosensor for Environmental Monitoring: Recent Advances and Perspectives

“…Carbon dot–based DNA nanosensor has been used for detection of mutagenic nitrosamines, namely N -nitrosodimethylamine and N -nitrosodiethanolamine. Chitosan carbon dot–modified glassy carbon electrode immobilized with DNA undergoes change in differential pulse voltammetry peak current in presence of nitrosamines [ 132 ] (Fig. 8 ).…”

“…8 Schematic representation of the process of fabricating modified electrode and subsequent detection of nitrosamine. Reproduced with permission from Majumdar et al, American Chemical Society [ 132 ] …”

Property of carbon dots and their applications in biosensor

“…Majumdar等 [104] 制造了基于DNA-碳点的电化学 [38] . 图 13 用于腺苷检测的化学发光传感器的检测机制 [101] (网 络版彩图) Figure 13 Detection mechanism of chemiluminescence sensor for adenosine detection [101] (color online).…”

“…参考文献 图 16 修饰电极的制作过程和亚硝胺的后续检测 [104] (网络 版彩图) Figure 16 The manufacturing process of the modified electrode and the subsequent detection of nitrosamine [104] (color online).…”