DNA conformational equilibrium in the presence of Zn2+ ions in neutral and alkaline solutions

Sorokin, V. A.; Valeev, Vladimir A.; Usenko, E. L.; Andrushchenko, Valery

doi:10.1016/j.ijbiomac.2011.11.011

Cited by 12 publications

(12 citation statements)

References 17 publications

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“…There are few significant reasons that prompted us to use these buffers in the study. First of all, Mes, Pipes, and Caco are widely used in biological experiments as well as in calorimetric studies and are capable to maintain a pH of 6. Moreover, the binding constants ( K MB ) and the enthalpies of complexation (Δ H o MB ) of cobalt(II) and nickel(II) ions with Mes, Pipes, and Caco are known .…”

Section: Resultsmentioning

confidence: 99%

Simultaneous determination of thermodynamic and kinetic parameters of aminopolycarbonate complexes of cobalt(II) and nickel(II) based on isothermal titration calorimetry data

Tesmar

Wyrzykowski

Muñoz³

et al. 2016

J of Molecular Recognition

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The influence of the different side chain residues on the thermodynamic and kinetic parameters for complexation reactions of the Co and Ni ions has been investigated by using the isothermal titration calorimetry (ITC) technique supported by potentiometric titration data. The study was concerned with the 2 common tripodal aminocarboxylate ligands, namely, nitrilotriacetic acid and N-(2-hydroxyethyl) iminodiacetic acid. Calorimetric measurements (ITC) were run in the 2-(N-morpholino)ethanesulfonic acid hydrate (2-(N-morpholino) ethanesulfonic acid), piperazine-N,N'-bis(2-ethanesulfonic acid), and dimethylarsenic acid buffers (0.1 mol L , pH 6) at 298.15 K. The quantification of the metal-buffer interactions and their incorporation into the ITC data analysis enabled to obtain the pH-independent and buffer-independent thermodynamic parameters (K, ΔG, ΔH, and ΔS) for the reactions under study. Furthermore, the kinITC method was applied to obtain kinetic information on complexation reactions from the ITC data. Correlations, based on kinetic and thermodynamic data, between the kinetics of formation of Co and Ni complexes and their thermodynamic stabilities are discussed.

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Section: Resultsmentioning

confidence: 99%

Simultaneous determination of thermodynamic and kinetic parameters of aminopolycarbonate complexes of cobalt(II) and nickel(II) based on isothermal titration calorimetry data

Tesmar

Wyrzykowski

Muñoz³

et al. 2016

J of Molecular Recognition

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“…These buffer substances are widely used in biological experiments as well as in calorimetric studies (Good et al, 1966;Christensen et al, 2003;Wyrzykowski and Chmurzyński, 2010;Deegan et al, 2011;Wyrzykowski et al, 2011;Ghai et al, 2012;Sorokin et al, 2012). However, to the best of our knowledge, there are few reports on the interactions of such buffers with the zinc ions that are omnipresent in biological systems (Vallee and Auld, 1990;Dudev and Lim, 2000).…”

Section: Introductionmentioning

confidence: 99%

Zinc(II) complexation by some biologically relevant pH buffers

Wyrzykowski

Tesmar

Jacewicz

et al. 2014

J of Molecular Recognition

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The isothermal titration calorimetry (ITC) technique supported by potentiometric titration data was used to study the interaction of zinc ions with pH buffer substances, namely 2-(N-morpholino)ethanesulfonic acid (Mes), piperazine-N,N'-bis(2-ethanesulfonic acid) (Pipes), and dimethylarsenic acid (Caco). The displacement ITC titration method with nitrilotriacetic acid as a strong, competitive ligand was applied to determine conditional-independent thermodynamic parameters for the binding of Zn(II) to Mes, Pipes, and Caco. Furthermore, the relationship between the proposed coordination mode of the buffers and the binding enthalpy has been discussed.

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“…Divalent metal ions play an important role in the functionality and the conformation stability of biopolymers [6]. e numerous data on metal complexes of the natural DNA and double-stranded homopolynucleotides containing pairs of canonical nitrogenous bases (adenine-thymine and guanine-cytosine) are already available [1,[7][8][9][10][11][12][13][14][15][16]. It is known that the main binding sites of metal ions to polynucleotides are oxygen atoms of phosphate groups, as well as nitrogen or oxygen atoms of nitrogenous bases [10,[12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…e numerous data on metal complexes of the natural DNA and double-stranded homopolynucleotides containing pairs of canonical nitrogenous bases (adenine-thymine and guanine-cytosine) are already available [1,[7][8][9][10][11][12][13][14][15][16]. It is known that the main binding sites of metal ions to polynucleotides are oxygen atoms of phosphate groups, as well as nitrogen or oxygen atoms of nitrogenous bases [10,[12][13][14]. It is shown that alkali and alkaline earth metal ions interact predominantly with phosphate groups resulting in stabilization of the structure of nucleic acids.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Divalent Metal Ions on the Temperature Stability of Poly(I:C) Duplex

Usenko

Valeev

Glamazda

et al. 2020

Journal of Spectroscopy

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The effect of divalent ions on the thermal stability of the double-stranded polynucleotide polyinosinic:polycytidylic acid (poly(I:C)) in a buffer solution (pH 7) was studied by UV absorption spectroscopy and thermal denaturation by measuring the absorbance at 260 nm. The concentration dependence of the polynucleotide melting temperature (Tm) in the presence of metal ions (2.5 × 10−6–10−2 M) was obtained. It was found that the duplex thermal stability grew gradually upon an increase of Ca2+ and Mn2+ concentration. The thermal stability of the duplex in the presence of Cd2+, Ni2+, and Zn2+ rises up to an ion concentration of about 10−4 M and further falls. In contrast to these ions, the addition of Cu2+ to the solution leads to a sharp decline in Тm of the duplex at a concentration above 10−5 M. The observed difference in the influence of metal ions on the poly(I:C) thermal stability is explained by the different affinity of these ions to both the phosphate groups and the nitrogenous bases of the polynucleotides. Based on the analysis of the Tm concentration dependence, the dominant binding of each ion to the specific polynucleotide components was proposed.

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DNA conformational equilibrium in the presence of Zn2+ ions in neutral and alkaline solutions

Cited by 12 publications

References 17 publications

Simultaneous determination of thermodynamic and kinetic parameters of aminopolycarbonate complexes of cobalt(II) and nickel(II) based on isothermal titration calorimetry data

Simultaneous determination of thermodynamic and kinetic parameters of aminopolycarbonate complexes of cobalt(II) and nickel(II) based on isothermal titration calorimetry data

Zinc(II) complexation by some biologically relevant pH buffers

The Effect of Divalent Metal Ions on the Temperature Stability of Poly(I:C) Duplex

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