DNA damage induced by micellar-delivered doxorubicin and ultrasound: comet assay study

Husseini, Ghaleb A.; El-Fayoumi, Refaat Ibrahim; O’Neill, Kim L.; Rapoport, Natalya; Pitt, William G.

doi:10.1016/s0304-3835(00)00399-2

Cited by 67 publications

(65 citation statements)

References 17 publications

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“…These vesicles appear to prevent general and/or premature release of the drug [37][38][39]. In theory, liposomes and micelles should not be acoustically active if they do not contain any gas.…”

Section: Types Of Drug Carriersmentioning

confidence: 99%

Ultrasonic drug delivery – a general review

Pitt

Husseini

Staples

2004

Expert Opinion on Drug Delivery

557

428

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Ultrasound (US) has an ever-increasing role in the delivery of therapeutic agents including genetic material, proteins, and chemotherapeutic agents. Cavitating gas bodies such as microbubbles are the mediators through which the energy of relatively non-interactive pressure waves is concentrated to produce forces that permeabilize cell membranes and disrupt the vesicles that carry drugs. Thus the presence of microbubbles enormously enhances delivery of genetic material, proteins and smaller chemical agents. Delivery of genetic material is greatly enhanced by ultrasound in the presence of microbubbles. Attaching the DNA directly to the microbubbles or to gas-containing liposomes enhances gene uptake even further. US-enhanced gene delivery has been studied in various tissues including cardiac, vascular, skeletal muscle, tumor and even fetal tissue. US-enhanced delivery of proteins has found most application in transdermal delivery of insulin. Cavitation events reversibly disrupt the structure of the stratus corneum to allow transport of these large molecules. Other hormones and small proteins could also be delivered transdermally. Small chemotherapeutic molecules are delivered in research settings from micelles and liposomes exposed to ultrasound. Cavitation appears to play two roles: it disrupts the structure of the carrier vesicle and releases the drug; it also makes the cell membranes and capillaries more permeable to drugs. There remains a need to better understand the physics of cavitation of microbubbles and the impact that such cavitation has upon cells and drug-carrying vesicles.

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Section: Types Of Drug Carriersmentioning

confidence: 99%

Ultrasonic drug delivery – a general review

Pitt

Husseini

Staples

2004

Expert Opinion on Drug Delivery

557

428

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“…However, to our knowledge, most of them are used to detect the related phenomena. For example, the biomedical ultrasound can damage the genes [1]; it also can improve the gene transfection and curative effects of gene therapy via enhancing the permeability of cells [2]. As for the proteins macromolecules, biomedical ultrasound can reduce its activity and lead to its denaturalization.…”

Section: Introductionmentioning

confidence: 99%

Apoptosis induced by low-intensity ultrasound in vitro: Alteration of protein profile and potential molecular mechanism

Feng¹,

Wan²

2017

AIP Conference Proceedings

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Abstract. To analyze the potential mechanism related to the apoptosis induced by low intensity focused ultrasound, comparative proteomic method was introduced in the study. After ultrasound irradiation (3.0 W/cm 2 , 1 minute, 6 hours incubation post-irradiation), the human SMMC-7721 hepatocarcinoma cells were stained by trypan blue to detect the morphologic changes, and then the percentage of early apoptosis were tested by the flow cytometry with double staining of FITC-labelled Annexin V/Propidium iodide. Two-dimensional SDS polyacrylamide gel electrophoresis was used to get the protein profile and some proteins differently expressed after ultrasound irradiation were identified by MALDI-TOF mass spectrometry. It's proved early apoptosis of cells were induced by low intentisy focused ultrasound. After ultrasound irradiation, the expressing characteristics of several proteins changed, in which protein p53 and heat shock proteins are associated with apoptosis initiation. It is suggested that the low-intensity ultrasound-induced apoptotic cancer therapy has the potential application via understanding its relevant molecular signaling and key proteins. Moreover, the comparative proteomic method is proved to be useful to supply information about the protein expression to analyze the metabolic processes related to bio-effects of biomedical ultrasound.

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“…Our previous studies confirmed the hypothesis that Dox was encapsulated in Pluronic P105 micelles and released upon exposure to ultrasound. Such ultrasonically controlled release has been effective against cancer cells in vitro [6] and in vivo [7,8].…”

Section: Introductionmentioning

confidence: 99%

The role of cavitation in acoustically activated drug delivery

Husseini

Rosa

Richardson

et al. 2005

Journal of Controlled Release

Self Cite

156

139

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Pluronic P105 micelles are potential candidates as chemotherapy drug delivery vehicles using ultrasonic stimulation as a release trigger. Acoustic power has been previously shown to release two anthracycline agents from these polymeric carriers. In this study, an ultrasonic exposure chamber with fluorescence detection was used to examine the mechanism of doxorubicin release from P105 micelles. Acoustic spectra were collected and analyzed, at the same spatial position as fluorescence data, to probe the role of cavitation in drug release. Our study showed a strong correlation between percent drug release and subharmonic acoustic emissions, and we attribute the drug release to collapse cavitation that perturbs the structure of the micelle and releases drug.

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DNA damage induced by micellar-delivered doxorubicin and ultrasound: comet assay study

Cited by 67 publications

References 17 publications

Ultrasonic drug delivery – a general review

Ultrasonic drug delivery – a general review

Apoptosis induced by low-intensity ultrasound in vitro: Alteration of protein profile and potential molecular mechanism

The role of cavitation in acoustically activated drug delivery

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