DNA detection of Gyrodactylus spp. in skin mucus of Nile tilapia Oreochromis niloticus

Ek-Huchim, Juan Pablo; Jiménez-García, Isabel; Rodríguez-Canul, Rossanna

doi:10.1016/j.vetpar.2019.07.004

Cited by 5 publications

(4 citation statements)

References 19 publications

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“…Molecular identification of gyrodactylid and dactylogyrid monogeneans is largely based on nuclear ribosomal DNA (rDNA) markers. 310 Ek-Huchim et al 311,312 designed primer combinations within the nuclear rDNA for non-invasive identification of monogeneans on tilapia.…”

Section: Researchmentioning

confidence: 99%

“…and dactylogyrids is time consuming and requires detailed morphometric analysis of microscopic structures of the parasite attachment and/or copulatory organs and specialist knowledge: a practical alternative in aquatic veterinary medicine could be screening fish mucus using PCR to identify parasite molecular markers. 312 The relevance of this approach is limited to situations where precise taxonomic identification is required to meet quarantine regulations for export permits, or where parasite life-cycles are well documented and approaches to strategic control are established. Most reports of farmed tilapia mortality, furthermore, are associated with G. cichlidarum.…”

Section: Researchmentioning

confidence: 99%

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A global review of problematic and pathogenic parasites of farmed tilapia

Shinn

Avenant‐Oldewage

Bondad‐Reantaso

et al. 2023

Reviews in Aquaculture

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Over the past 80 years, tilapia have been translocated globally for aquaculture; active production is recorded in >124 countries. Of 7 million tonnes of tilapia produced in aquaculture, 79% is from 79 countries outside the natural range of tilapia. Capture fisheries account for a further 723,627 tonnes of tilapia, and >47% of this is landed from established invasive populations outside Africa. Tilapias host a rich fauna of parasites, many of which have been translocated with their hosts. This review summarises >2500 host–parasite records from 73+ countries and >820 recorded tilapia translocations (provided in the supplementary materials). This work focuses on the notable pathogens that threaten the health of cultured populations of tilapia, providing a description of their pathology and includes species that also have substantial impacts on wild tilapia populations, where relevant. For each major parasite taxonomic group, we highlight which parasites have been translocated or have been acquired from the new environments into which tilapia have been introduced, together with remarks on standard treatment approaches and research on them and their management and control. Regarding the theme ‘Tilapia health: quo vadis?’, Africa has enormous potential for aquaculture growth, but substantial knowledge gaps about tilapia parasites in many African states remain, which creates associated production and biosecurity risks. For each parasitic group, therefore, the risks of parasite translocation to new regions as tilapia aquaculture industries expand are highlighted.

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Section: Researchmentioning

confidence: 99%

Section: Researchmentioning

confidence: 99%

A global review of problematic and pathogenic parasites of farmed tilapia

Shinn

Avenant‐Oldewage

Bondad‐Reantaso

et al. 2023

Reviews in Aquaculture

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“…This parasite has been recorded in several farmed and wild cichlids in Africa, including banded jewelfish Hemichromis fasciatus in Senegal 25 , Nile tilapia O. niloticus in Kenya 26 and Mozambique tilapia O. mossambicus in Madagascar 27 ; and outside Africa, it has been recorded on all continents except Antarctica 6 , 7 , 11 . Gyrodactylus cichlidarum is widely distributed in Mexico and infects not only farmed and feral “tilapia” throughout the country 12 – 17 , 28 , 29 , but has also been recorded on native poeciliid fishes 19 . This survey corroborates that this translocated African parasite is found throughout the country infecting several species/strains of “tilapia”, including O. niloticus , O. mossambicus , O. aureus , “Rocky mountain”, “Pargo UNAM” 30 and “Florida tilapia”; and provides the first records of infection of three native cichlid fishes in Mexico: P. nebuliferus and V. fenestrata (both in Oaxaca) and an unidentified native cichlid collected in Chiapas.…”

Section: Discussionmentioning

confidence: 99%

Morpho-molecular characterization of Gyrodactylus parasites of farmed tilapia and their spillover to native fishes in Mexico

García‐Vásquez

Pinacho-Pinacho

Guzmán‐Valdivieso

et al. 2021

Sci Rep

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Translocation of fishes for aquaculture has resulted in the co-introduction of some of their parasites. African cichlid fishes, generically called “tilapias” have been introduced worldwide, along with their monogenean parasites. In a nation-wide survey, we characterised monogeneans of the genus Gyrodactylus infecting farmed “tilapia” throughout Mexico. We also collected native fishes around farms, to look for potential parasite spillover from cultured fishes. Monogeneans were identified taxonomically using morphological and molecular characters. Originally African, pathogenic Gyrodactylus cichlidarum was recorded in every farm surveyed, infecting different “tilapia” varieties, as well as three native cichlid fish species. Previously, we had shown that G. cichlidarum also infects native, non-cichlid fishes in Mexico. We also recorded that Gyrodactylus yacatli is widely distributed in Mexico, infecting cultured “tilapia” and native fishes; and present data indicating that this is a further translocated African parasite. A third, unidentified gyrodactylid infected farmed and native fishes in Chiapas, southern Mexico; we describe the new species as Gyrodactylus shinni n. sp., and provide evidence that this is a third monogenean translocated with African fish. The wide distribution of exotic parasites co-introduced with “tilapia” and their spillover to native fishes may have an important impact on the ichthyofauna in Mexico, one the world’s megadiverse countries.

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“…5) showed a low yield of PCR product in comparison with the other 26 samples. The fish skin mucus swabbing method had been used in aquaculture to detect an aquaculture-related gene [23] and pathogen [24], however, our swabbing together with the disc method is much simpler and faster than any of the available procedures. Compared to the drawing of blood, the sampling process by swabbing was efficient and non-invasive.…”

Section: Efficacy Of Swabbing and Disc Technique For Fish Dna Samplingmentioning

confidence: 99%

Low-Cost and Rapid Method of DNA Extraction from Scaled Fish Blood and Skin Mucus

Liu

Lin

et al. 2022

Viruses

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PCR-based DNA amplification has been one of the major methods in aquaculture research for decades, although its use outside the modern laboratory environment is limited due to the relatively complex methods and high costs. To this end, we investigated a swabbing and disc protocol for the collection of DNA samples from fish which could extract DNA from fish skin mucus by a non-invasion technique costing only $0.02 (USD) and requiring less than 30 seconds. The disc method that we chose could use the cheap filter paper to extract DNA from above 104 crucian carp blood cells, which is comparable to the commercial kit. By using skin mucus swabbing and the disc method, we can obtain amplification-ready DNA from mucus to distinguish different species from our smallest fish (medaka, ~2.5 cm and crucian carp, ~7 cm) to our biggest fish (tilapia, ~15 cm). Furthermore, the viral pathogen Carassius auratus herpesvirus (CaHV) of crucian carp was detected using our method, which would make performing molecular diagnostic assays achievable in limited-resource settings including aquafarms and aqua stores outside the laboratory environment.

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DNA detection of Gyrodactylus spp. in skin mucus of Nile tilapia Oreochromis niloticus

Cited by 5 publications

References 19 publications

A global review of problematic and pathogenic parasites of farmed tilapia

A global review of problematic and pathogenic parasites of farmed tilapia

Morpho-molecular characterization of Gyrodactylus parasites of farmed tilapia and their spillover to native fishes in Mexico

Low-Cost and Rapid Method of DNA Extraction from Scaled Fish Blood and Skin Mucus

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