DNA Diagnostics to Identify Internal Feeders (Lepidoptera: Tortricidae) of Pome Fruits of Quarantine Importance

Bárcenas, Nina M.; Unruh, Thomas R.; Neven, Lisa

doi:10.1603/0022-0493-98.2.299

Cited by 26 publications

(17 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The oriental fruit moth is a major pest of stone and pome fruit in many fruit‐growing regions of the world (Il'ichev et al., ; Natale et al., ; Barcenas et al., ; Myers et al., ; Chen & Dorn, ). It can produce 3–7 generations per year depending on the latitude or elevation (Barnes, ).…”

Section: Introductionmentioning

confidence: 99%

Population genetics affected by pest management using fruit‐bagging: a case study with Grapholita molesta in China

Zheng

Qiao

Wang

et al. 2015

Entomologia Exp Applicata

View full text Add to dashboard Cite

Fruit-bagging is being used as a pest management tool to control fruit moths on several continents. However, nothing is known about how this physical control method affects population genetics of these moths. Focusing on the oriental fruit moth, Grapholita (= Cydia) molesta (Busck) (Lepidoptera: Tortricidae), we used five polymorphic microsatellite loci and two mitochondrial gene sequences to investigate the genetic diversity and genetic structure of populations from three fruit-bagged and two unbagged apple orchards in five regions of a major fruit-growing area of China. We sampled each orchard 39 during the season and found high genetic diversity in the samples of populations collected. Populations from fruit-bagged and unbagged orchards were genetically significantly differentiated, indicating a clear effect of the pest management method applied. Genetic structures of the populations under a given management regime changed in the course of the season in a characteristic manner, and within the same regime, patterns were always similar at a given sampling time. There was no significant isolation by geographical distance. Population genetics from both orchard types did not resemble one another at the beginning of the season, but they did rather at the end. Potential reasons for this unexpected direction of change in population genetic structure are discussed, and we hypothesize that diapause-related characteristics may account for the patterns observed. The current study documents that pest management methods applied can have an important impact on the genetic diversity and genetic structure of targeted pest insect populations.

show abstract

Section: Introductionmentioning

confidence: 99%

Population genetics affected by pest management using fruit‐bagging: a case study with Grapholita molesta in China

Zheng

Qiao

Wang

et al. 2015

Entomologia Exp Applicata

View full text Add to dashboard Cite

show abstract

“…Moreover, DNA sequencing involves additional cost and time for quarantine work. Some recent studies have suggested that PCR with species-specific primers could overcome the need of DNA sequencing and accelerate the process of identification (Barcenas et al 2005;Rugman-Jones et al 2009;Zhang et al 2012;Jiang et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Rapid diagnosis of the invasive wax scale, Ceroplastes rusci Linnaeus (Hemiptera: Coccoidea: Coccidae) using nested PCR

Deng

Wang

et al. 2014

J Applied Entomology

View full text Add to dashboard Cite

The fig wax scale, Ceroplastes rusci (Linnaeus) (Hemiptera: Coccoidea: Coccidae), is an invasive fruit pest of Afrotropical origin and potentially could become a serious threat to commercial fruit crops in China. C. rusci is difficult to identify owing to the shortage of easily distinguishable morphological characters. A rapid, accurate and reliable method to identify C. rusci in quarantine work is needed to detect further spread. In the present study, we describe a nested PCR method for the molecular identification of C.rusci. The nested PCR primers were designed based on variations in the barcode region of COI sequences between C. rusci and five other Ceroplastes species. A 200‐bp fragment was successfully amplified from 96 C. rusci individuals of seven geographical populations in China and Vietnam, and 13 individuals of two populations in Italy (the type country for C. rusci). These provided diagnostic bands that were not observed in any of five other Ceroplastes species widely distributed in China, namely, C. ceriferus (Fabricius), C. floridensis Comstock, C. japonicus Green, C. pseudoceriferus Green and C. rubens Maskell. Sensitivity tests revealed that diagnostic bands were generated even with a DNA template concentration of ~1.5 × 10−5 ng/μl, and with average DNA template concentrations for adult females, single first‐instar nymphs and eggs of 14.7, 6.3 and 3.0 ng/μl, respectively. Our study demonstrates that the molecular diagnosis of C. rusci using nested PCR is rapid and accurate and shows potential in plant quarantine programmes.

show abstract

“…Additionally, Yvon et al (2009) discriminated virus variants and showed that HRM and standard melt results were 100% identical for c. 450 samples. Standard melt peak analysis has also been used to discriminate mammalian carnivores (Berry & Sarre 2007), insect quarantine pests (Barcenas, Unruh, & Neven 2005;Yu et al 2005) and fungi (Hsu et al 2003). However, with each of these analyses, different primer sets were used to amplify each of the known haplotypes within the populations under study.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of DNA melting analysis as a tool for species identification

et al. 2010

View full text Add to dashboard Cite

Summary 1.One of the ongoing challenges in molecular biology is to develop rapid, accurate and reliable techniques to identify organisms. This contribution evaluates the value of DNA melt peak analysis for the identification of pests and pathogens significant to biosecurity. 2. The method evaluated in this study capitalises on the observation that double-stranded DNA separates into single strands (melts) at a temperature dependant on the nucleotide sequence of the DNA strands. The temperature and shape of melt peaks from polymerase chain reaction (PCR) products using standard primers and amplification protocols were evaluated for species identification, and the advantages of this method over agarose gel electrophoresis exemplified. 3. Three insect (weevil) species were discriminated by the melt profile shape and temperature of their cytochrome oxidase subunit I (COI) PCR product using order-specific universal primers. 4. Three of four arachnid (tick) species were discriminated by the melt profile shape and temperature of their COI and 18S PCR product using order-specific universal primers. 5. Plum pox virus-specific primers amplified only the target virus that was verified by its unique melt temperature rather than by conventional staining of the amplicon on an electrophoretic gel. 6. Melt peak analysis is a useful and robust tool for the rapid detection of PCR products and the identification of organisms. An efficiency study revealed this technique required half the time input, and cost half as much, as PCR followed by AGE.

show abstract

DNA Diagnostics to Identify Internal Feeders (Lepidoptera: Tortricidae) of Pome Fruits of Quarantine Importance

Cited by 26 publications

References 29 publications

Population genetics affected by pest management using fruit‐bagging: a case study with Grapholita molesta in China

Population genetics affected by pest management using fruit‐bagging: a case study with Grapholita molesta in China

Rapid diagnosis of the invasive wax scale, Ceroplastes rusci Linnaeus (Hemiptera: Coccoidea: Coccidae) using nested PCR

Evaluation of DNA melting analysis as a tool for species identification

Contact Info

Product

Resources

About