DNA-Directed Assembly of Carbon Nanotube–Protein Hybrids

Freeley, Mark; Gwyther, Rebecca E. A.; Jones, D. Dafydd; Palma, Matteo

doi:10.3390/biom11070955

Cited by 10 publications

(17 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, all steps have their own challenges and limitations, but the recent advances show specific routes and the large potential. Another direction is multicolor NIR labels as shown by the assembly of chirality-pure carbon nanotube-protein hybrids . In addition to such multicolor NIR imaging, , the benefits of monodispersity on biocompatibility and toxicity could become one of the central advantages for most applications.…”

Section: Challenges and Outlookmentioning

confidence: 99%

“…Another direction is multicolor NIR labels as shown by the assembly of chirality-pure carbon nanotube-protein hybrids. 96 In addition to such multicolor NIR imaging, 69,97 the benefits of monodispersity on biocompatibility and toxicity could become one of the central advantages for most applications. One example was shown by Galassi and colleagues, reporting the high biocompatibility of ssDNA-(9,4) SWCNTs for in vivo mice studies.…”

Section: ■ Challenges and Outlookmentioning

confidence: 99%

See 1 more Smart Citation

Prospects of Fluorescent Single-Chirality Carbon Nanotube-Based Biosensors

Nißler

Ackermann

et al. 2022

Anal. Chem.

View full text Add to dashboard Cite

Semiconducting single-wall carbon nanotubes (SWCNTs) fluoresce in the near-infrared (NIR), and the emission wavelength depends on their structure (chirality). Interactions with other molecules affect their fluorescence, which has successfully been used for SWCNT-based molecular sensors. So far, most such sensors are assembled from crude mixtures of different SWCNT chiralities, which causes polydisperse sensor responses as well as spectral congestion and limits their performance. The advent of chirality-pure SWCNTs is about to overcome this limitation and paves the way for the next generation of biosensors. Here, we discuss the first examples of chirality-pure SWCNT-based fluorescent biosensors. We introduce routes to such sensors via aqueous two-phase extraction-assisted purification of SWCNTs and highlight the critical interplay between purification and surface modification procedures. Applications include the NIR detection and imaging of neurotransmitters, reactive oxygen species, lipids, bacterial motives, and plant metabolites. Most importantly, we outline a path toward how such monodisperse (chirality-pure) sensors will enable advanced multiplexed sensing with enhanced bioanalytical performance.

show abstract

Section: Challenges and Outlookmentioning

confidence: 99%

Section: ■ Challenges and Outlookmentioning

confidence: 99%

Prospects of Fluorescent Single-Chirality Carbon Nanotube-Based Biosensors

Nißler

Ackermann

et al. 2022

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…This increase in the diameter seems to be the result of protein adsorption onto the DNA-SWNTs . If proteins and SWNTs are bound by DNA owing to the electron coupling effect, the fluorescence intensity will increase . This may be the reason why the absorbance and PL enhancements of DNA-SWNTs were higher when the DNA-SWNTs were mixed with the banana solution because it was speculated that the absorbance and PL intensity would be increased remarkably when banana-binding proteins were adhered to the DNA-SWNTs.…”

Section: Resultsmentioning

confidence: 99%

Distinguishing Antioxidant Molecules with Near-Infrared Photoluminescence of DNA-Wrapped Single-Walled Carbon Nanotubes

et al. 2022

View full text Add to dashboard Cite

In this study, two biomolecule solutions were distinguished using the capacity difference in the near-infrared photoluminescence (PL) of single-walled carbon nanotubes (SWNTs). Biosensing techniques using sensitive responses of SWNTs have been intensively studied. When a small amount of an oxidant or reductant solution was injected into the SWNT suspensions, the PL intensity of the SWNTs is significantly changed. However, distinguishing between different molecules remains challenging. In this study, we comparably injected saponin and banana solutions, which are known antioxidant chemicals, into an SWNT suspension. The SWNTs were solubilized by wrapping them with DNA molecules. The results show that 69.1 and 155.2% increases of PL intensities of SWNTs were observed after injection of 20 and 59 μg/mL saponin solutions, respectively. Subsequently, the increase in PL was saturated. With the banana solution, 18.1 and 175.4% increases in PL intensities were observed with 20 and 59 μg/mL banana solutions, respectively. Based on these results, the two antioxidant molecules could be distinguished based on the different PL responses of the SWNTs. In addition, the much higher saturated PL intensities observed with the banana solution suggests that the banana solution increased the capacity of the PL increase for the same SWNT suspension. These results provide helpful information for establishing biosensing applications of SWNTs, particularly for distinguishing chemicals.

show abstract

“…The inherent structure of the polymeric nucleic acid, in particular, complements SWCNTs; with planar nucleobases forming π–π stacking interactions with the sidewalls, and the polar sugar‐phosphate backbone providing sufficient torsion to wrap the nanotube [169] . The natural amphiphilic structure of ssDNA means it is also often employed in the preliminary stages of SWCNT functionalisation, to aid dispersion and control the available surface area for protein functionalisation [170,171] …”

Section: Functionalisation Of Nanocarbon For Biosensing Applicationsmentioning

confidence: 99%

“…In terms of its applications, ssDNA use in NC‐FET design is expansive, being used directly as a DNA probe to sense for target sequences and also indirectly, with additional chemistries being incorporated to create a platform for further biomolecule attachment [171,172] . For example, research by Xu et al .…”

Section: Functionalisation Of Nanocarbon For Biosensing Applicationsmentioning

confidence: 99%

Fabrication and Functionalisation of Nanocarbon‐Based Field‐Effect Transistor Biosensors

et al. 2022

Self Cite

View full text Add to dashboard Cite

Nanocarbon-based field-effect transistor (NC-FET) biosensors are at the forefront of future diagnostic technology. By integrating biological molecules with electrically conducting carbon-based platforms, high sensitivity real-time multiplexed sensing is possible. Combined with their small footprint, portability, ease of use, and label-free sensing mechanisms, NC-FETs are prime candidates for the rapidly expanding areas of point-of-care testing, environmental monitoring and biosensing as a whole. In this review we provide an overview of the basic operational mechanisms behind NC-FETs, synthesis and fabrication of FET devices, and developments in functionalisation strategies for biosensing applications.

show abstract

DNA-Directed Assembly of Carbon Nanotube–Protein Hybrids

Cited by 10 publications

References 45 publications

Prospects of Fluorescent Single-Chirality Carbon Nanotube-Based Biosensors

Prospects of Fluorescent Single-Chirality Carbon Nanotube-Based Biosensors

Distinguishing Antioxidant Molecules with Near-Infrared Photoluminescence of DNA-Wrapped Single-Walled Carbon Nanotubes

Fabrication and Functionalisation of Nanocarbon‐Based Field‐Effect Transistor Biosensors

Contact Info

Product

Resources

About