DNA extraction and purification from archival material: Revisiting the standard operating procedure

Netto, Matias Schadeck; Alves, Abraão Leal; Carvalho, E.F.; Garrido, Rodrigo Grazinoli; Silva, Dayse A.

doi:10.1016/j.fsigss.2015.09.227

Cited by 2 publications

(2 citation statements)

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“…The results of all the samples that were washed in ethanol only once demonstrated, without exception, considerable interference by proteins and phenol. In addition, the extracted DNA may display PCR inhibitors, similar to what was observed by Netto et al (2015). Furthermore, a DNA amount much greater than the ideal was observed in the quantification tests, affirming the need for serial dilutions to achieve ideal values of around 0.5 and 1.0 ng DNA/µL before PCR amplification.…”

Section: Discussionsupporting

confidence: 72%

Research Article In vitro recovery and identification of Y-STR DNA from Chrysomya albiceps ( Diptera, Calliphoridae) larvae fed a decomposing mixture of human semen and ground beef

Chamoun¹,

Couri²,

Louro³

et al. 2019

Genet. Mol. Res.

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Worldwide, several women become victims of rape every day. Many of those women are also murdered, with their bodies sometimes being found in an advanced state of decomposition, resulting in loss of evidence important to criminal investigations. Diptera is one of the main orders associated with human body decomposition. Fly species that belong to the family Calliphoridae are usually scavengers and are frequently found on ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 18 (1): gmr18189 C.A. Chamoun et al. 2 decomposing bodies, thereby playing an important role in forensic entomology. The recovery and genotyping of human Y-STR DNA from the gastrointestinal contents of the calliphorid Chrysomya albiceps larvae has promising applications in the investigation of sexual crimes, such as rape, and in cases of murder and abandonment of the victim's body, which may be found in a state of decomposition. We studied this species of fly with the aim of supporting such investigations. After establishment of a colony, larvae were fed with decomposing human semen mixed in ground bovine meat (1 mL per 200 g beef). Larvae (10-15) were collected every 24 h and kept in 70% ethanol, to give a total of 96 larvae obtained after eight days of decomposition. The digestive system of each larva was resected. Molecular typing was conducted, which comprised sample extraction, quantification, amplification, and capillary electrophoresis with 16 STR loci from the Y chromosome. We succeeded in establishing a Y-STR DNA profile, with amplification of up to 11 loci, from individual samples, or up to 15 loci, when a combination of samples corresponding to the time-points 48, 72, 120, 144, and 192 h was used.

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Section: Discussionsupporting

confidence: 72%

Research Article In vitro recovery and identification of Y-STR DNA from Chrysomya albiceps ( Diptera, Calliphoridae) larvae fed a decomposing mixture of human semen and ground beef

Chamoun¹,

Couri²,

Louro³

et al. 2019

Genet. Mol. Res.

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“…This may be related to either DNA absorption or retention by each swab type, which varies considerably [19]. In this study, we only used organic extraction, which had its efficiency proved for low DNA‐content samples in a previous study [20]. Therefore, further studies should be carried out with different extraction methods so that the performance of different swabs on the same surfaces (gearshift knob, steering wheel, and parking brake lever) could be assessed.…”

Section: Discussionmentioning

confidence: 99%

Touch DNA recovery from vehicle surfaces using different swabs

Giovanelli

Garrido

Rocha

et al. 2021

Journal of Forensic Sciences

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Several methods of DNA collection are used in places or objects related to crimes, the most common being the use of swabs. However, it is known that the efficacy of touch DNA recovery can be affected by collection devices and surfaces. The aim of this work was to evaluate the efficiency of three different types of swab in recovering touch DNA collected from different parts of a vehicle. The following swabs were tested: PurFlock® swab (Puritan, USA), 4N6FLOQSwabs™ (Copan S.p.A., Italy), and cotton swab (Labor Import). The experiments were carried out in the same vehicle, using the gearshift knob, the parking brake lever, and the steering wheel as support for the collection of touch DNA. Swabs showed significant differences in the amount of DNA recovered (Hc = 53.52; p < 0.05) and in the rate of allele amplification (Hc = 24.3; p < 0.05). The results indicated a greater DNA recovery efficiency by PurFlock® swab, followed by cotton, and then 4N6FLOQSwabs™. However, there was no significant difference among the surfaces analyzed. PurFlock® swab was more efficient for recovering donor alleles than the others (cotton and 4N6FLOQSwabs™), especially for small DNA amounts. This swab was, therefore, suitable for collections in vehicles involved in crime. Furthermore, this study highlights the need to assess different materials and methods of collection of biological samples, considering collection, extraction, and amplification.

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DNA extraction and purification from archival material: Revisiting the standard operating procedure

Cited by 2 publications

References 8 publications

Research Article <i>In</i> <i>vitro</i> recovery and identification of Y-STR DNA from <i>Chrysomya</i> <i>albiceps</i> ( Diptera, Calliphoridae) larvae fed a decomposing mixture of human semen and ground beef

Research Article <i>In</i> <i>vitro</i> recovery and identification of Y-STR DNA from <i>Chrysomya</i> <i>albiceps</i> ( Diptera, Calliphoridae) larvae fed a decomposing mixture of human semen and ground beef

Touch DNA recovery from vehicle surfaces using different swabs

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