Dna Extraction Methods for Fin of Mackerel in Thailand

Abstract

The quality and quantity of DNA are crucial aspects for fish studies based on molecular techniques. The extraction method of genomic DNA is depended on tissue types and organism species. This research aimed to identify an appropriate extraction method for gDNA from the fin of three popular mackerel species in Thailand; short-bodied mackerel (Rastrelliger brachysoma), island mackerel (R. faughni) and Indian mackerel (R. kanagurta). Four different methods for gDNA extraction were compared based on time, quality and quantity of extractable gDNA for PCR technique. Method III showed the highest quantity of gDNA in R. brachysoma and R. kanagurta. Nevertheless, the highest purity of gDNA for both species was obtained by method II and IV, respectively. The gDNA from method IV was successful to amplify the intense band of β-actin fragment. The highest concentration and purity of gDNA from R. faughni were received using method II. However, β-actin gene fragment amplified from gDNA of method IV showed intense bands. These results indicated that method IV was suitable for gDNA extraction from the fin of three mackerel species because of the fastest, high quality and quantity for PCR amplification.

Studies on the Genus Garra (Teleostei: Cyprinidae) from the Kaladan Drainage of Mizoram, India, and Synonymization of G. tyao with G. rakhinica

“…Total DNA extraction from muscle tissue of specimens of was performed using the Phenol Chloroform Isoamyl alcohol method following Makkapan et al (15) . Amplification of the cytochrome c oxidase subunit I (COI) gene was performed using ProFlex PCR System.…”