DNA-fingerprinting (AFLP and RFLP) for genotypic identification in species of the Pleurotus eryngii complex

Abstract

Wild populations of edible species are important source of genetic variability for cultivated lines that can undergo a drastic loss of diversity resulting from man's selection. The development of tools aimed at the clear-cut and safe identification and assessment of genetic variability of the wild and cultivated strains is thus a fundamental goal of molecular genetic research. In this study, we used two polymerase chain reaction (PCR)-based fingerprinting methods-amplified fragment length polymorphism (AFLP) and restriction fragment length polymorphism (RFLP) of laccase and manganese peroxidase genes-to assess genetic differences among strains and independently evolving lineages belonging to the Pleurotus eryngii complex. Both laccase RFLP and AFLP have been proved to distinguish unambiguously the three taxa studied: Pleurotus ferulae, P. eryngii, and P. eryngii var. nebrodensis. AFLP also showed enough sensitivity to detect polymorphisms among the strains, proving to be an efficient DNA fingerprinting tool in studies of strain assignment. The divergent RFLP laccase and manganese peroxidase patterns are also discussed in relation to the role played by these genes in the interaction between these fungi and their host plants.

The complete mitochondrial genome of the Basidiomycete edible fungus Pleurotus eryngii

“…Pleurotus eryngii is an edible mushroom with a high commercial value and has been widely cultivated in Europe, Middle East, North America, and Asia (Mandeel et al 2005). The genetic diversity and population structure of P. eryngii have been investigated in several studies (Urbanelli et al 2003(Urbanelli et al , 2007Abdollahzadeh et al 2007;Ro et al 2007;Zervakis et al 2014). However, little is known about the mitochondrial genome of this fungus.…”

Evaluation of the genetic diversity of Bupleurum using amplified fragment length polymorphism analysis

“…This substantial divergence observed within B. falcatum L. highlights the difficulty of traditional variety identification using phenotypes alone. The addition of molecular markers could help determine not only the source of some plants but also provide information necessary for the identification and optimization of plant varieties (Urbanelli et al, 2007;Abdul Wahab et al, 2014).…”

Development of AFLP markers and phylogenetic analysis in Hypsizygus marmoreus

“…Developing and genotyping AFLP markers have been reported in several fungi (Boucias et al, 2000;Majer et al, 1996;Purwantara et al, 2000). The technique has also been applied to genotypic identifi cation in only a few edible mushrooms such as Lentinula edodes (Terashima and Matsumoto, 2004;Terashima et al, 2002) and Pleurotus eryngii (Urbanelli et al, 2007). However, to our best knowledge, there is no report that developed a reliable experimental protocol for genotyping H. marmoreus with AFLP.…”