DNA fingerprinting by pulsed field gel electrophoresis and ribotyping to distinguish Pseudomonas cepacia isolates from a nosocomial outbreak

Anderson, Daniel J.; Kuhns, Janet; Vasil, Michael L.; Gerding, DN; Janoff, Edward N.

doi:10.1128/jcm.29.3.648-649.1991

Cited by 81 publications

(20 citation statements)

References 9 publications

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“…Ribotyping and DNA fingerprinting by PFGE could successfully differentiate strains isolated from the different hospitals. Ribotyping and DNA fingerprinting by PFGE are a powerful marker for classifying B. cepacia, as reported previously (2,18). A discrepancy between ribotyping and DNA fingerprinting by PFGE was observed in only three strains tested in the present study.…”

Section: Discussionsupporting

confidence: 63%

Analysis of strains of Burkholderia (Pseudomonas) cepacia isolated in a nosocomial outbreak by biochemical and genomic typing

et al. 1995

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We analyzed strains of Burkholderia (formerly Pseudomonas) cepacia isolated in a nosocomial outbreak by biochemical and genomic typing methods. One hundred isolates of B. cepacia were obtained from patients at several wards in a single hospital from March 1983 to February 1984. These isolates were classified into 12 groups by a new biochemical typing scheme on the basis of the production of six enzymes and the production of hemolytic substance and yellow pigment. Among them, 33 strains collected from the 12 groups were further characterized by DNA fingerprinting by pulse-field gel electrophoresis, ribotyping, and plasmid profile analysis. Forty-seven strains of B. cepacia of independent origins and 25 isolates from the same hospital obtained 10 years later for a follow-up study were also subjected to analysis. Both DNA fingerprinting and ribotyping clearly discriminated the isolates from different hospitals. Of interest, although the biochemical typing and plasmid profiles of the isolates obtained during 1983 to 1984 in a single hospital were variable, genomic typing identified the majority of the isolates (32 of 33 [97%]) as derivatives of a single strain. Furthermore, a follow-up study suggested the persistence of such derivatives among the isolates after a decade. These results clearly indicated that the outbreak of B. cepacia infection in the hospital was nosocomial in origin. Thus, the usefulness of genomic typing for epidemiological studies of B. cepacia infection was further demonstrated. The biochemical typing revealed the marked variability of phenotypes of B. cepacia.

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Section: Discussionsupporting

confidence: 63%

Analysis of strains of Burkholderia (Pseudomonas) cepacia isolated in a nosocomial outbreak by biochemical and genomic typing

et al. 1995

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“…The epidemiology, diversity, and population structure of a number of pathogenic bacteria have been studied by several techniques that include serological analysis (9), analysis of whole-cell (6) and outer membrane protein (9) profiles, multilocus enzyme electrophoresis (36,37,43,44), restriction endonuclease cleavage of chromosomal DNA (6,51), and the detection of specific restriction fragment length polymorphisms (RFLPs) at specific loci or within rRNA operons (2,4,6,24,48,49). These techniques have been extremely valuable in identifying strains responsible for nosocomial infections and their transmission and maintenance in patients and in studies of bacterial pathogenesis.…”

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confidence: 99%

Clonal diversity of Streptococcus mitis biovar 1 isolates from the oral cavity of human neonates

Fitzsimmons

Evans

Pearce

et al. 1996

Clin Diagn Lab Immunol

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The clonal diversity of 101 isolates of the pioneer bacterium Streptococcus mitis biovar 1 obtained from the oral cavities of 40 human neonates 1 to 3 days, 2 weeks, and 1 month postpartum was examined by using rRNA gene restriction patterns. There was a high degree of genetic diversity, with the 101 isolates comprising 93 unique PvuII ribotypes. There were eight identical pairs of ribotype patterns, and seven of the eight pairs were obtained from individual neonates. Only one identical pair comprised isolates obtained from different neonates. In all but two cases, isolates with matching ribotypes were obtained at one visit. Two pairs of isolates with matching ribotype patterns were obtained from neonates on successive visits. The ribotype patterns of the isolates were examined by cluster analysis. The isolates forming each cluster were very similar, yet each cluster was well separated from its neighbors. When several isolates were obtained from individual neonates at a particular visit, in some instances they were contained in a single cluster, whereas in other cases each isolate was contained in a separate cluster. Isolates obtained from individual neonates on successive visits tended to be contained in different clusters. This high degree of diversity, which has been observed in other mucosal commensal bacteria, may serve as a mechanism for avoiding immune elimination of these bacteria.

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“…Genotypic characterization of strains may circumvent these difficulties. Indeed, analysis of the restriction fragment length polymorphism of total DNA by pulsed-field gel electrophoresis (PFGE) and analysis of the ribosomal DNA regions (ribotyping) have both been shown to be effective in distinguishing among P. cepacia strains (1,20).…”

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confidence: 99%

Arbitrarily primed polymerase chain reaction as a rapid method to differentiate crossed from independent Pseudomonas cepacia infections in cystic fibrosis patients

et al. 1993

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We used DNA fingerprinting by the arbitrarily primed polymerase chain reaction (AP-PCR) technique for an epidemiological investigation of 23 Pseudomonas cepacia isolates obtained from 11 cystic fibrosis (CF) patients attending our CF center. This approach was compared with ribotyping, pulsed-field gel electrophoresis (PFGE), and conventional phenotypic typing. AP-PCR and ribotyping were identical in resolving power, since the two methods generated four different profiles and identified the same group of strains. Six patients on the one hand and four on the other harbored strains of the same genotype, thus raising the possibility of either patient-to-patient transmission or acquisition from a common hospital environmental source. PFGE results were in good agreement with those of the other two methods, but PFGE seems more discriminative since it

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DNA fingerprinting by pulsed field gel electrophoresis and ribotyping to distinguish Pseudomonas cepacia isolates from a nosocomial outbreak

Cited by 81 publications

References 9 publications

Analysis of strains of Burkholderia (Pseudomonas) cepacia isolated in a nosocomial outbreak by biochemical and genomic typing

Analysis of strains of Burkholderia (Pseudomonas) cepacia isolated in a nosocomial outbreak by biochemical and genomic typing

Clonal diversity of Streptococcus mitis biovar 1 isolates from the oral cavity of human neonates

Arbitrarily primed polymerase chain reaction as a rapid method to differentiate crossed from independent Pseudomonas cepacia infections in cystic fibrosis patients

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