DNA Fingerprinting of Jute Germplasm by RAPD

Abstract

The genotype characteristic of cultivars was investigated, along with varieties of both of the jute species, Corchorus olitorius and Corchorus capsularis, in the germplasm collection at the Bangladesh Jute Research Institute (BJRI). DNA fingerprinting was generated for 9 different varieties and 12 accessions of jute cultivars by using random amplified polymorphic DNA (RAPD). A total of 29 arbitrary oligonucleotide primers were screened. Seven primers gave polymorphism within the varieties, and 6 primers detected polymorphism within the accessions that were tested. A dendrogram was engendered from these data, and this gave a distinct clustering of the cultivated species of jute. Therefore, we generated RAPD markers, which are species-specific. These primers can distinguish between C. olitorius and C. capsularis. From the dendrogram that we generated between the various members of these two species, we found the existing genetic classification that agrees with our molecular marking data. A different dendrogram showed that jute accessions could be clustered into three groups. These data will be invaluable in the conservation and utilization of the genetic pool in the germplasm collection.

Determining Genetic Diversity of Some Jute Varieties and Accessions Using RAPD Markers

“…2) was constructed using the programme STATISTICA, version 3.0 (Stat Soft 1994), based on the genetic distance matrix. The relationship that is portrayed by this clustering also agreed with the available pedigree information (Hossain et al 2002). Two major clusters representing two species were resolved among the genotypes that were examined in the study.…”

“…along with varieties were incorporated in this study. A similar study was made only with eight Bangladeshi jute varieties (Hossain et al 2002). In the present study 10 more genotypes (2 new varieties and 8 accessions) were compared with 8 varieties to determine the relative genetic distance within species.…”

“…However, very little molecular information of jute or its related species is available at the gene bank, and very few efforts have been made in the past to develop molecular markers to study its genetic variability (Hossain et al 2002, Roy et al 2006, Basu et al 2004). In the previous studies different authors determined the genetic variability of different jute varieties and accessions (collected from various diverse locations) using RAPD and AFLP (Hossain et al 2002(Hossain et al , 2003, STMS, ISSR and RAPD (Roy et al 2006) and AFLP and SSR markers (Basu et al 2004). The accessions possessing unique agronomic traits such as snow-white fibre, short field duration (40 -60 days), low temperature tolerance, waterlogging tolerance etc.…”

SSR markers linked to mite (Polyphagotarsonemus latus Banks) resistance in jute (Corchorus olitorius L.)

“…PCR based markers have been successfully developed such as RFLPs, RAPDs and SSRs (Bradshaw et al 1994;Cheung et al 1997;Jung et al 1999). These markers have been successfully exploited in many crops including jute (Hossain et al 2002(Hossain et al , 2003Qi et al 2003;Basu et al 2004;Roy et al 2006;Akter et al 2008) for genetic diversity analysis as well as genotyping. Among these markers, simple sequence repeats (SSR) have become the markers of choice due to several desirable attributes including their abundance, multiallelic and codominant nature, high level of reproducibility, cross-species transferability etc.…”

Construction of Genetic Map of Jute (Corchorus olitorius L.) Based on RAPD Markers

“…(BJRI). So far very little efforts have been undertaken in the past to develop molecular markers to study its genetic variability (Hossain et al 2002, 2003, Basu et al 2004, Roy et al 2006, Mir et al 2008, Akter et al 2008. So generation of information at the molecular level would be helpful for its improvement.…”