DNA Fingerprints in Plants Using Simple-sequence Repeat and Minisatellite Probes

Abstract

<jats:p>Plant genomes contain polymorphic repetitive sequences that can be used as DNA markers. Minisatellites (16 to 64 bp per repeat) and simple-sequence repeats (2 to 6 bp per repeat) are the most polymorphic markers found in plant and animal genomes. In this study, the hybridizations between genomic DNA and variable number of tandem repeat probes were examined in <jats:italic>Arabidopsis thaliana</jats:italic> L. (Heynn)<jats:italic>,</jats:italic> onion (<jats:italic>Allium cepa</jats:italic> L.), tomato (<jats:italic>Lycopersicon esculentum</jats:italic> L.), wheat (<jats:italic>Triticum aestivum</jats:italic> L.), avocado (<jats:italic>Persea americana</jats:italic> Mill.), litchi (<jats:italic>Chinensis</jats:italic> Sonn.), mango (<jats:italic>Mangifera indica</jats:italic> L.), and <jats:italic>Carica</jats:italic> species. Some of the probes detected polymorphic sequences in all the species, but others were useful only for one or two species. None of the probes gave clear band patterns in either onion or wheat. The in-gel hybridization method was similar to Southern blot hybridization using the simple-sequence repeat probes.</jats:p>

Generation and analysis of 9792 EST sequences from cold acclimated oat, Avena sativa

“…SSRs have therefore been widely utilized in plant genomic studies [33-36]. They are especially advantageous when there is a need to track desirable traits in large-scale breeding programs and when defining anchor points for map-based gene cloning strategies.…”

Molecular markers in Allium .

“…DNA fingerprinting has earlier been called the hybridization of a genomic DNA blot with labelled microsatellite sequences, which makes corresponding target sequences visible throughout the genome (see Bruford et al, 1992, for an overview). The technique has been tried on A. cepa without success (Sharon et al, 1995;M. Klaas, unpublished results), even though the presence of corresponding sequences in A. cepa has been demonstrated by the successful generation of microsatellite markers (Fischer and Bachmann, 1998).…”

RAPD based genetic variability among cultivated varieties of Aonla (Indian Gooseberry, Phyllanthus emblica L.)

“…Warude et al (2006) have reported on the development and application of RAPD-SCAR marker for the identification of Phyllanthus emblica (at the species level), however studies on the genetic variability of Aonla varieties employing molecular markers have not been attempted. It is very important to characterize the genetic variation using fool-proof methods such as DNA fingerprinting (Sharon et al, 1995) that can generate vital information on the varietal authentication especially in processing as this fruit has a prominent place in neutraceutical industry (Pathak, 2003). This study also finds immense application at the field level by way of The data presented here provide possibilities of identifying and differentiating most commonly cultivated varieties of P. emblica using 4 commercially available primers.…”