DNA hybridization detection on electrical microarrays using coulostatic pulse technique

Dharuman, Venkataraman; Nebling, Eric; Grünwald, Thomas; Albers, Jörg; Blohm, Lars; Elsholz, Bruno; Wörl, Ralf; Hintsche, R.

doi:10.1016/j.bios.2006.02.014

Cited by 24 publications

(11 citation statements)

References 54 publications

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“…As may be expected, while hybridization kinetics of the free (non-SWCNT bound) DNA strands were very fast -occurring in less than 10 min -the kinetics of the SWCNT-bound DNA strand were considerably slower, reaching completion only after 13 h at 25 °C. A detection limit of 6 nM was observed, which is comparable with electrical microarray techniques [93], but higher than more recently techniques that utilize surface initiated enzymatic polymerization [94].…”

Section: Dna Hybridizationmentioning

confidence: 60%

Biomedical Application of Carbon Nanotubes for Proteins Extraction and Seperation

Schlüter

Saboktakin²

2016

J. Pharm. Nutr. Sci.

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Measurement science and technology continue to play vital roles in biomedical research and in routine healthcare. Over recent decades there has been a steady evolution of sensors for biomedical measurement aimed at clinical care in hospitals, fundamental biomedical research in the laboratory, or even self-care in the home. The measurements of interest are diverse, ranging from pressure, force, flow and displacement to electrical field/charge, magnetic flux, and molecular species, such as gases, ions, proteins, bacteria, viruses, and DNA. In this review, we have studied several biomedical applications of nanotubes and nanowires for proteins measurements in cells. Also, These materials have a wide application as protein carriers and transporters. The wide applications of multi-walled carbon nanotubes (MWCNT) on the serious concerns about their safety on human health and environment have been studied.

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Section: Dna Hybridizationmentioning

confidence: 60%

Biomedical Application of Carbon Nanotubes for Proteins Extraction and Seperation

Schlüter

Saboktakin²

2016

J. Pharm. Nutr. Sci.

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“…In this method, schematized in Figure 2, a double potential step is applied from an initial potential E i = 0 V, to a final potential E f = η, with respect to the open circuit potential value; then, after 20 ms, the potential is returned to its initial value [25]. The potential η corresponds to the formal potential of the redox couple used as an indicator, while on the other hand η is a potential value small enough that the system has a virtually linear I-E behavior.…”

Section: Methodsmentioning

confidence: 99%

DNA Biosensor Based on Double-Layer Discharge for the Detection of HPV Type 16

Espinosa

Galván

Quiñones

et al. 2019

Sensors

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DNA electrochemical biosensors represent a feasible alternative for the diagnosis of different pathologies. In this work, the development of an electrochemical method for Human Papillomavirus-16 (HPV-16) sensing is reported based on potential relaxation measurements related to the discharge of a complex double layer of a DNA-modified gold electrode. The method used allows us to propose an equivalent circuit (EC) for a DNA/Au electrode, which was corroborated by electrochemical impedance spectroscopy (EIS) measurement. This model differs from the Randles circuit that is commonly used in double-layer simulations. The change in the potential relaxation and associated charge transfer resistance were used for sensing the DNA hybridization by using the redox pair Fe(CN)64-/Fe(CN)63+ as an electrochemical indicator. In order to determinate only the potential relaxation of the composed double layer, the faradic and double-layer current contributions were separated using a rectifier diode arrangement. A detection limit of 0.38 nM was obtained for the target HPV-16 DNA sequences. The biosensor showed a qualitative discrimination between a single-base mismatched sequence and the fully complementary HPV-16 DNA target. The results indicate that the discharge of the double-layer detection method can be used to develop an HPV DNA biosensor.

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“…The amount of enzymes G enz linked to the hybridized sample can be finally calculated from the generation rate J by Equation 6 J ¼ k cat G enz (6) where k cat is the turnover number for b-galactosidase using PAPG as substrate. The turnover number can be determined be measuring the flux W of PAP molecules produced from a well defined number of b-galactosidase molecules in the regime of substrate saturation.…”

Section: Determination Of the Accessible Odn Surfacementioning

confidence: 99%

Scanning Electrochemical Microscopy (SECM) Based Detection of Oligonucleotide Hybridization and Simultaneous Determination of the Surface Concentration of Immobilized Oligonucleotides on Gold

2007

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International audienceThe direct mode of scanning electrochemical microscopy (SECM) was used for the local deposition of oligonucleotide (ODN) patterns on thin gold films and the generation-collection (GC) mode was applied for the determining the amount of surface-accessible oligonucleotides. The local deposition was achieved through the micrometer-sized formation of a conducting polymer bearing 15^mer single-stranded oligonucleotide strands. After the interaction of the oligonucleotide with its biotin-labeled complimentary strand, streptavidin was bound. The molecular assembly was completed by linking biotin-labeled -galactosidase from Escherichia coli to the streptavidin. The activity of the linked β-galactosidase was mapped with SECM in the GC mode by monitoring the oxidation of p-aminophenol (PAP) formed in the enzyme-catalyzed hydrolysis of p-aminophenyl-β-D-galactopyranoside. The feedback effect due to recycling of the reaction product at the gold surface was analyzed. It was shown experimentally that this effect becomes insignificant at ultramicroelectrode (UME)-substrate distances larger than 3 UME radii. The flux of formed PAP allowed the determination the surface density of accessible oligonucleotide strands in the functionalized polymer. It was shown that that thicker pyrrole/ODN-Pyrrole polymer films do not lead to a significantly increased accessible ODN surface concentration

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DNA hybridization detection on electrical microarrays using coulostatic pulse technique

Cited by 24 publications

References 54 publications

Biomedical Application of Carbon Nanotubes for Proteins Extraction and Seperation

Biomedical Application of Carbon Nanotubes for Proteins Extraction and Seperation

DNA Biosensor Based on Double-Layer Discharge for the Detection of HPV Type 16

Scanning Electrochemical Microscopy (SECM) Based Detection of Oligonucleotide Hybridization and Simultaneous Determination of the Surface Concentration of Immobilized Oligonucleotides on Gold

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