DNA hydrolysis promoted by 1,7-dimethyl-1,4,7,10-tetraazacyclododecane

Design of artificial nucleases and studies of their interaction with DNA

“…Hence, it is an important task to look for small organic molecules which can cleave nucleic acids via hydrolysis and transphosphorylation. Reported results have shown that some small organic molecules, such as guanidine derivatives [15−17] , cyclodextrin derivatives [39,40] , macrocyclic polyamines [41] and peptides [42] , can cleave active phosphate diester and RNA. Göbel et al [43,44] put forward the concept of "metal free catalysis" and synthesized compounds 22a and 22b containing three guanidine side arms, which can catalyze RNA hydrolysis without metal ions.…”

Synthesis and Biological Studies of N‐Alkylated Cyclic Diamines

“…We tried to recover the linear DNA from an agarose low-melting-point gel by cutting off a gel fragment and subjecting it to a DNA recovering system for small-scale gels. Then, the linear DNA recovered was subjected to an overnight ligation reaction with T4 DNA ligase [19]. The result of electrophoresis (Fig.…”

“…The soln. containing DNA recovered was incubated with 1 ml of T4 DNA ligase and 2 ml of ligation buffer for 12 h at 208 [19]. In control experiments, the T4 ligase reactions were performed also on pBR322 previously linearized with EcoR I.…”

The Effect of an Amino‐Acid Bridge on Binding Affinity and Cleavage Efficiency of Pyrenyl‐Macrocyclic Polyamine Conjugates toward DNA

“…Recently, our group has reported several cyclen derivatives, which were used to mimic natural nucleases and to catalyze the cleavge of DNA [22 -24]. Comparing to metal -cyclen complexes, free 1,7-dimethylcyclen (DMC) also displayed good DNA cleavage ability [25]. We report here, for the first time, the design and synthesis of a series of novel amino acid-bridged cyclen-pyrene compounds.…”