DNA hydrolysis promoted by di- and multi-nuclear metal complexes

Liu, Changlin; Wang, Ming; Zhang, Tianle; Sun, Hongzhe

doi:10.1016/j.cct.2003.11.002

Cited by 443 publications

(178 citation statements)

References 105 publications

(147 reference statements)

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“…[4,5] Among the different therapeutic strategies to eradicate cancer cells through DNA damage, the view of using small water-soluble transition-metal complexes, capable of oxidative or hydrolytic DNA cleavage as anti-cancer drugs, is a challenging topic in bioinorganic chemistry. [6,7] Many transition-metal complexes with V, [8] Fe, [9] Cu, [10,11] Co, [12] lanthanides, [13,14] and also actinides [15] have been reported as efficient DNA cleaving agents with or without sequence specificity. Of these metals, zinc is the second most abundant transition-metal ion present in the human body, [16,17] and is in the active sites of several enzymes such as superoxide dismutase, proteins containing zinc finger motifs and zinc hydrolases, in particular, owing to its Lewis acid character.…”

Section: Introductionmentioning

confidence: 99%

Unique Ligand‐Based Oxidative DNA Cleavage by Zinc(II) Complexes of Hpyramol and Hpyrimol

Maheswari

Barends

Özalp-Yaman

et al. 2007

Chemistry A European J

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The zinc(II) complexes reported here have been synthesised from the ligand 4‐methyl‐2‐N‐(2‐pyridylmethyl)aminophenol (Hpyramol) with chloride or acetate counterions. All the five complexes have been structurally characterised, and the crystal structures reveal that the ligand Hpyramol gradually undergoes an oxidative dehydrogenation to form the ligand 4‐methyl‐2‐N‐(2‐pyridylmethylene)aminophenol (Hpyrimol), upon coordination to ZnII. All the five complexes cleave the ϕX174 phage DNA oxidatively and the complexes with fully dehydrogenated pyrimol ligands were found to be more efficient than the complexes with non‐dehydrogenated Hpyramol ligands. The DNA cleavage is suggested to be ligand‐based, whereas the pure ligands alone do not cleave DNA. The DNA cleavage is strongly suggested to be oxidative, possibly due to the involvement of a non‐diffusible phenoxyl radical mechanism. The enzymatic religation experiments and DNA cleavage in the presence of different radical scavengers further support the oxidative DNA cleavage by the zinc(II) complexes.

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Section: Introductionmentioning

confidence: 99%

Unique Ligand‐Based Oxidative DNA Cleavage by Zinc(II) Complexes of Hpyramol and Hpyrimol

Maheswari

Barends

Özalp-Yaman

et al. 2007

Chemistry A European J

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“…In fact, interaction of the substrate with two electrophilic metal centers favors the nucleophilic attack of a Zn-OH function and thus enhances the rate of the hydrolytic process, as already found in several dinuclear synthetic Zn(II) complexes [33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50]. As discussed above, in the dinuclear L2 complexes the two metals can attain a short intermetallic distance, favoring a bridging interaction mode of the phosphate ester.…”

Section: Conversely the Monohydroxo Complex [Zn 2 L2(oh)]mentioning

confidence: 98%

“…BNPP hydrolysis promoted by mono-and dinuclear Zn(II) complexes is generally explained in terms of an "associative" mechanism [33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50]71], in which the substrate approaches the Zn(II) complex and the oxygens of BNPP start associating with the electrophilic Zn(II) ion. A zincbound hydroxide operates a simultaneous nucleophilic attack to the phosphorus atom.…”

Section: Bis(p-nitrophenyl)phosphate Hydrolysismentioning

confidence: 99%

Zn(II) Coordination by Open-chain Polyamine Ligands Containing Dipyridine Units. Highly Active Dinuclear Zn(II) Complexes in Phosphate Ester Hydrolysis

Bazzicalupi¹,

Bencini

Danesi³

et al. 2008

TOICJ

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Abstract:The synthesis and characterization of two new open-chain ligands, L1 and L2, containing two 2,2'-dipyridine units connected respectively by a pentaethylenhexamine and a pentapropylenhexamine chain, is reported. Zn(II) complexation has been studied by means of potentiometric and UV-vis spectrophotometric measurements in aqueous solution. Both ligands form stable mono-and dinuclear complexes, the metal ions being coordinated by a single dipyridine unit and by the adjacent amine groups. The ability of the binuclear Zn(II) complexes as nucleophilic agents in hydrolytic processes has been tested by using bis(p-nitrophenyl) phosphate (BNPP) as substrate. Both complexes display high rate enhancements in BNPP cleavage in aqueous solution. The study outlines that different mechanisms are operative in BNPP cleavage in the presence of the L1 and L2 complexes.

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“…For these reasons, a powerful tool for the manipulation of large DNA has been strongly desired. [1][2][3][4][5][6][7][8] Recently, we have developed artificial restriction DNA cutter (ARCUT) for site-specific hydrolysis of double-stranded DNA. [9][10][11][12] This new tool is composed of Ce(IV)/EDTA complex, which selectively hydrolyzes single-stranded portion in double-stranded DNA, and a pair of pseudo-complementary peptide nucleic acid (pcPNA).…”

Section: Introductionmentioning

confidence: 99%

Site-specific gene cassette insertion by combining artificial restriction DNA cutter and single-stranded DNA specific endonuclease

Katada¹,

Shigi²,

Komiyama³

2008

Arkivoc

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An artificial restriction DNA cutter (ARCUT), which was composed of Ce(IV)/EDTA complex and a pair of pseudo-complementary peptide nucleic acid, was used for construction of recombinant plasmid vector. The plasmid vector was cut by ARCUT only at target site, and resultant specific overhangs were blunted by treatment with Mung Bean Nuclease and Klenow fragment. This fragment having blunt ends at both termini was ligated with the gene of enhanced green fluorescent protein (EGFP) prepared by PCR. The resultant recombinant vector was translated and the fluorescent protein was successfully expressed in cells. Throughout the manipulation, neither natural restriction enzymes nor homologous recombination was employed. Thus, this strategy has promising applicability to construction of still larger vectors.

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DNA hydrolysis promoted by di- and multi-nuclear metal complexes

Cited by 443 publications

References 105 publications

Unique Ligand‐Based Oxidative DNA Cleavage by Zinc(II) Complexes of Hpyramol and Hpyrimol

Unique Ligand‐Based Oxidative DNA Cleavage by Zinc(II) Complexes of Hpyramol and Hpyrimol

Zn(II) Coordination by Open-chain Polyamine Ligands Containing Dipyridine Units. Highly Active Dinuclear Zn(II) Complexes in Phosphate Ester Hydrolysis

Site-specific gene cassette insertion by combining artificial restriction DNA cutter and single-stranded DNA specific endonuclease

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