DNA immobilization, delivery and cleavage on solid supports

Singh, Vikram; Zharnikov, Michael; Gulino, Antonino; Gupta, Tarkeshwar

doi:10.1039/c0jm04359a

Cited by 27 publications

(12 citation statements)

References 179 publications

(246 reference statements)

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“…Here, we briefly discussed some key aspects relevant to SiNW FET sensors. Comprehensive reviews are available for antibodies [ 135 , 136 ], enzymes [ 137 ], DNA [ 138 ] and aptamers [ 139 ]. Monoclonal and polyclonal antibodies are commonly used in SiNW FET biosensing, for instance for the detection of protein biomarkers [ 111 , 140 ], virus [ 97 , 141 , 142 ] and small molecules [ 143 ].…”

Section: Sensing Performance: Finding the Best Compromise Among Simentioning

confidence: 99%

CMOS-Compatible Silicon Nanowire Field-Effect Transistor Biosensor: Technology Development toward Commercialization

et al. 2018

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Owing to their two-dimensional confinements, silicon nanowires display remarkable optical, magnetic, and electronic properties. Of special interest has been the development of advanced biosensing approaches based on the field effect associated with silicon nanowires (SiNWs). Recent advancements in top-down fabrication technologies have paved the way to large scale production of high density and quality arrays of SiNW field effect transistor (FETs), a critical step towards their integration in real-life biosensing applications. A key requirement toward the fulfilment of SiNW FETs’ promises in the bioanalytical field is their efficient integration within functional devices. Aiming to provide a comprehensive roadmap for the development of SiNW FET based sensing platforms, we critically review and discuss the key design and fabrication aspects relevant to their development and integration within complementary metal-oxide-semiconductor (CMOS) technology.

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Section: Sensing Performance: Finding the Best Compromise Among Simentioning

confidence: 99%

CMOS-Compatible Silicon Nanowire Field-Effect Transistor Biosensor: Technology Development toward Commercialization

et al. 2018

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“…The macromolecular structure of DNA, its ability to hybridize, the diversity of non-natural nucleotides with unique functional groups that are available to perform chemistry on it, and enzymes that are capable of manipulating its sequence, structure, and topology provide rich opportunities for its use in clinical diagnostics, biosensors, gene therapy, and drug delivery. [1][2][3] Some of these applications rely on the immobilization of single-stranded DNA (ssDNA) onto a solid support, which is subsequently used for binding and detection of its complementary ssDNA target or for the recognition of DNA binding proteins. [4][5][6] A commonly used method for immobilization of ssDNAs is to functionalize them with a terminal reactive group that is selective for the surface of interest.…”

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confidence: 99%

Fabrication of ssDNA/Oligo(ethylene glycol) Monolayers and Complex Nanostructures by an Irradiation‐Promoted Exchange Reaction

et al. 2012

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Creative design: An approach to preparing mixed monolayers of thiolated single‐stranded DNA (ssDNA) and oligo(ethylene glycol)s (OEG‐AT) in a broad range of compositions as well as ssDNA/OEG‐AT patterns of any required shape (see top figure) has been shown. A combination of this approach with surface‐initiated enzymatic polymerization allows complex 3D DNA nanostructures to be sculpted with high spatial precision (bottom).

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“…In the last years, nucleic acids have been immobilized on surfaces, both noncovalently and covalently. 5 In this regard, noncovalent immobilization has been achieved by means of physical adsorption 6 and biospecific interactions (e.g. avidin-biotin).…”

Section: Introductionmentioning

confidence: 99%

Site-specific immobilization of DNA on silicon surfaces by using the thiol–yne reaction

et al. 2014

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Site-specific immobilization of DNA on silicon surfaces by thiol-yne reactionJorge Escorihuela, María-José Bañuls, Rosa Puchades and Ángel Maquieira* Covalent immobilization of ssDNA fragments onto silicon-based materials was performed using the thiol-yne reaction. Chemical functionalization provided alkyne groups on the surface where the thiol-modified oligonucleotide probes can be easily photoattached as microarrays, reaching an immobilization density around 30 pmol ·cm −2 . The developed method presents the advantages of spatially controlled probe anchoring (by using a photomask), direct attachment without using cross -linkers, and short irradiation times (20 min). Hybridization efficiencies up to 70%, with full complementar y strands, were reached. The approach was evaluated by scoring single nucleotide polymorphisms with a discrimination ratio around 15. Moreover, the potential applicability of the proposed methodology is demonstrated through the specific detection of 20 nM of a genomic target of bacterial Escherichia coli.

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DNA immobilization, delivery and cleavage on solid supports

Cited by 27 publications

References 179 publications

CMOS-Compatible Silicon Nanowire Field-Effect Transistor Biosensor: Technology Development toward Commercialization

CMOS-Compatible Silicon Nanowire Field-Effect Transistor Biosensor: Technology Development toward Commercialization

Fabrication of ssDNA/Oligo(ethylene glycol) Monolayers and Complex Nanostructures by an Irradiation‐Promoted Exchange Reaction

Site-specific immobilization of DNA on silicon surfaces by using the thiol–yne reaction

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