DNA insecticide developed from the Lymantria dispar 5.8S ribosomal RNA gene provides a novel biotechnology for plant protection

Oberemok, Volodymyr V.; Laikova, Kateryna V.; Gal'chinsky, Nikita V; Useinov, Refat Z.; Novikov, Ilya; Temirova, Zenure Z.; Shumskykh, Maksym N.; Krasnodubets, Alisa M.; Repetskaya, Anna Igorevna; Dyadichev, Valeriy V.; Фомочкина, И. И.; Bessalova, Evgenia Y.; Makalish, Tatiana P.; Gninenko, Yuri I.; Kubyshkin, Anatoly V.

doi:10.1038/s41598-019-42688-8

Cited by 24 publications

(40 citation statements)

References 44 publications

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Section: Introductionmentioning

confidence: 99%

“…To solve this problem, the development of DNA insecticides (oligonucleotide insecticides, or olinscides) is a promising direction of research [ 2 , 14 , 15 , 16 ]. The concentrations of modern chemical insecticides (organic xenobiotics) in ecosystems could be reduced by using DNA insecticides, which act on and are metabolized by ubiquitous DNAses [ 15 ]. Using short antisense fragments from the conserved regions of the genes of insect pests opens the possibility of using this approach when resistance to conventional insecticides occurs.…”

Section: Introductionmentioning

confidence: 99%

“…Oligonucleotide insecticides could be created by changing only the combination of nitrogenous bases depending on the gene sequences of the target insect pest. Our research suggests that antisense fragments of ribosomal genes are very promising for use as olinscides [ 14 , 15 , 16 ]. While mRNA comprises just 5% of cellular RNA, more abundant rRNA accounts for 80% of cellular RNA and is metabolically stable [ 17 , 18 ], making it an excellent candidate for silencing through antisense oligonucleotides.…”

Section: Introductionmentioning

confidence: 99%

“…While mRNA comprises just 5% of cellular RNA, more abundant rRNA accounts for 80% of cellular RNA and is metabolically stable [ 17 , 18 ], making it an excellent candidate for silencing through antisense oligonucleotides. Data from our recent studies show that DNA insecticides designed for gypsy moth larvae can be selective and thus non-harmful both for non-target insects, such as black cutworm and tobacco hornworm [ 19 ], and for plants such as wheat [ 20 ], oaks, and apple trees [ 15 , 21 ]. The striking selectivity of the action of oligonucleotide insecticides, shown in our recent experiments, when replacing only one nitrogenous base at the 5′- and 3′-ends of olinscides and dramatically reducing their efficiency, reveals great opportunities for creating well-tailored insecticides, which potentially have little to compare with in modern plant protection (under publication).…”

Section: Introductionmentioning

confidence: 99%

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Oligonucleotide Insecticides for Green Agriculture: Regulatory Role of Contact DNA in Plant–Insect Interactions

Oberemok

Useinov

Skorokhod

et al. 2022

IJMS

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Insects vastly outnumber us in terms of species and total biomass, and are among the most efficient and voracious consumers of plants on the planet. As a result, to preserve crops, one of the primary tasks in agriculture has always been the need to control and reduce the number of insect pests. The current use of chemical insecticides leads to the accumulation of xenobiotics in ecosystems and a decreased number of species in those ecosystems, including insects. Sustainable development of human society is impossible without useful insects, so the control of insect pests must be effective and selective at the same time. In this article, we show for the first time a natural way to regulate the number of insect pests based on the use of extracellular double-stranded DNA secreted by the plant Pittosporum tobira. Using a principle similar to one found in nature, we show that the topical application of artificially synthesized short antisense oligonucleotide insecticides (olinscides, DNA insecticides) is an effective and selective way to control the insect Coccus hesperidum. Using contact oligonucleotide insecticide Coccus-11 at a concentration of 100 ng/μL on C. hesperidum larvae resulted in a mortality of 95.59 ± 1.63% within 12 days. Green oligonucleotide insecticides, created by nature and later discovered by humans, demonstrate a new method to control insect pests that is beneficial and safe for macromolecular insect pest management.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Oligonucleotide Insecticides for Green Agriculture: Regulatory Role of Contact DNA in Plant–Insect Interactions

Oberemok

Useinov

Skorokhod

et al. 2022

IJMS

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“…In our past research with insects, 11-mer ASOs were found to be the most effective and produced optimal effects [44][45][46][47][48][49][50], which is why we used 11-mer phosphorothioate-modified Cytos-11 without methoxyethyl protection. To study its effects, it was administered twice a week for 21 days in a volume of 0.05 mL (3.5 mg/mL) via subplantar injection, followed by evaluation of the effects on the state of the joint and the concentration of TNF-α.…”

Section: Discussionmentioning

confidence: 99%

Anti-Rheumatic Effect of Antisense Oligonucleotide Cytos-11 Targeting TNF-α Expression

Makalish

Golovkin

Oberemok

et al. 2021

IJMS

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The urgency of the search for inexpensive and effective drugs with localized action for the treatment of rheumatoid arthritis continues unabated. In this study, for the first time we investigated the Cytos-11 antisense oligonucleotide suppression of TNF-α gene expression in a rat model of rheumatoid arthritis induced by complete Freund’s adjuvant. Cytos-11 has been shown to effectively reduce peripheral blood concentrations of TNF-α, reduce joint inflammation, and reduce pannus development. The results achieved following treatment with the antisense oligonucleotide Cytos-11 were similar to those of adalimumab (Humira®); they also compared favorably with those results, which provides evidence of the promise of drugs based on antisense technologies in the treatment of this disease.

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Post-genomic platform for development of oligonucleotide vaccines against RNA viruses: diamond cuts diamond

et al. 2022

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The coronavirus pandemic has starkly demonstrated the need to create highly effective vaccines against various viral diseases. The emerging new platforms for vaccine creation (adenovirus vectors and mRNA vaccines) have shown their worth in the fight against the prevention of coronavirus infection. However, adenovirus vectors and mRNA vaccines have a serious disadvantage: as a rule, only the S protein of the coronavirus is presented as an antigen. This tactic for preventing infection allows the ever-mutating virus to escape quickly from the immunity protection provided by such vaccines. Today, viral genomic databases are well-developed, which makes it possible to create new vaccines on a fundamentally new post-genomic platform. In addition, the technology for the synthesis of nucleic acids is currently experiencing an upsurge in demand in various fields of molecular biology. The accumulated experience suggests that the unique genomic sequences of viruses can act as antigens that trigger powerful humoral and cellular immunity. To achieve this effect, the following conditions must be created: the structure of the nucleic acid must be single-stranded, have a permanent 3D nanostructure, and have a unique sequence absent in the vaccinated organism. Oligonucleotide vaccines are able to resist the rapidly changing genomic sequences of RNA viruses by using conserved regions of their genomes to generate a long-term immune response, acting according to the adage that a diamond cuts a diamond. In addition, oligonucleotide vaccines will not contribute to antibody-dependent enhanced infection, since the nucleic acid of the coronavirus is inside the viral particle. It is obvious that new epidemics and pandemics caused by RNA viruses will continue to arise periodically in the human population. The creation of new, safe, and effective platforms for the production of vaccines that can flexibly change and adapt to new subtypes of viruses is very urgent and at this moment should be considered as a strategically necessary task.

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DNA insecticide developed from the Lymantria dispar 5.8S ribosomal RNA gene provides a novel biotechnology for plant protection

Cited by 24 publications

References 44 publications

Oligonucleotide Insecticides for Green Agriculture: Regulatory Role of Contact DNA in Plant–Insect Interactions

Oligonucleotide Insecticides for Green Agriculture: Regulatory Role of Contact DNA in Plant–Insect Interactions

Anti-Rheumatic Effect of Antisense Oligonucleotide Cytos-11 Targeting TNF-α Expression

Post-genomic platform for development of oligonucleotide vaccines against RNA viruses: diamond cuts diamond

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