DNA-mediated genetic correction of thiamineless Arabidopsis thaliana

Transfer of the human gene for hypoxanthine-guanine phosphoribosyltransferase via isolated human metaphase chromosomes into mouse L-cells.

“…Further experiments must clarify whether these results may be explained by evolutionary differences in the homology of DNA between donor and recipient cells. Our present data remind us of the results reported for the genetic transformation of the plant Arabidopsis thaliana (27) or the insect Drosophila melanogaster (28) where "exosome" models seem to best fit as an explanation of experimental results.…”

Fate of bacterial plasmid DNA during uptake by barley protoplasts

“…Some success has been reported [ 1,2] but the frequency of genetic transformation has been low and the results highly variable [3]. Plant cells devoid of cell walls (protoplasts) would seem to offer an advantage in such studies since they lack the natural barrier to uptake of DNAs, can be grown under selection conditions, and can be regenerated in some cases .…”

Detection by Agarose Gel Electrophoresis of Nucleases Associated with Cells and Protoplasts from Plant Supension Cultures UsingAgrobacterium tumefaciensTi Plasmid

“…l , 2) In spite of extensive investigations into the fate of exogenous DNA in plants, the nature and extent of uptake of intact DNA has not been elucidated and remains controversia1. 3 ,4) The availability of large quantities of isolated plant protoplasts provides material which is superior to whole plants for DNA uptake studies, particularly because protoplasts lack cell walls which may be a physical barrier for DNA passage into the cells, and because experiments can be readily carried out under aseptic conditions.…”