Transfer of the human gene for hypoxanthine-guanine phosphoribosyltransferase via isolated human metaphase chromosomes into mouse L-cells.

Willecke, Klaus; Ruddle, Frank H.

doi:10.1073/pnas.72.5.1792

Cited by 116 publications

(13 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The HRASI-selected transformants were analyzed for cotransfer of each of the nine gene markers shown in Fig. 1 (40b), based on the combined results from deletion and transl9cation mapping in hybrid cells (4,11,12,13,39,42), in situ hybridization analysis of mitotic chromosomes (44), and family linkage data with segregating polymorphic markers (15,41). Cells and culture conditions.…”

mentioning

confidence: 99%

Molecular and physical arrangements of human DNA in HRAS1-selected, chromosome-mediated transfectants.

Porteous¹,

Morten²,

Cranston³

et al. 1986

Mol. Cell. Biol.

View full text Add to dashboard Cite

We used mitotic chromosomes isolated from a human EJ bladder carcinoma cell line for morphological transformation of mouse C127 cells. These chromosome-mediated transformants were analyzed for cotransfer of markers syntenic with c-Ha-ras-1 on human chromosome 11. We also used cloned, dispersed human DNA repeats, in a general mapping strategy, to quantitate the amounts and molecular state of human DNA transferred along with the activated c-Ha-ras-1 gene. In situ hybridization was used to visualize the physical state of the transfected human chromatin. The combined use of these various techniques revealed the occurrence of both chromosomal and DNA rearrangements. However, our analysis also demonstrated that, in general, very substantial lengths of DNA are transferred intact. Closely linked markers are likely to cosegregate. Therefore, these transformants should be invaluable sources for the complete molecular cloning of isolated fragments of the short arm of human chromosome 11.CMGT is a long-established technique which enables variable subchromosomal lengths of DNA to be transferred efficiently to appropriate recipient cells (reviewed by McBride and Peterson [23]). Exploitation of this and other somatic cell techniques has been compromised by the limited availability of dominantly acting selection systems (23,31,39). We can now describe the use of a new class of dominant selectable markers in CMGT, the human oncogenes. Mitotic chromosomes derived from the human EJ bladder carcinoma cell line induced morphological transformation, anchorage-independent growth, and tumorigenicity in mouse C127 cells

show abstract

mentioning

confidence: 99%

Molecular and physical arrangements of human DNA in HRAS1-selected, chromosome-mediated transfectants.

Porteous¹,

Morten²,

Cranston³

et al. 1986

Mol. Cell. Biol.

View full text Add to dashboard Cite

show abstract

“…The initial success of chromosome transfer experiments was followed by the transfer of a variety of human and rodent genes into mutant recipient cell lines (24,25). The frequency of transfer, however, was very low in the presence of poly-lysine buffer used by McBride and Ozer (21).…”

Section: Chromosome Transfer In Mammalian Cellsmentioning

confidence: 99%

Monochromosomal Hybrids and Chromosome Transfer: A Functional Approach for Gene Identification

Kandpal

Sandhu

Kaur

et al. 2017

CGP

View full text Add to dashboard Cite

show abstract

“…In several other laboratories, it was confirmed that the genetic information from isolated mammalian chromosomes was transferred into recipient cells by the chromosome immigration method (McBride and Ozer, 1973;Shani et a[., 1974;Willecke and Ruddle, 1975;Burch and McBride, 1975;Shani et al, 1976). However, with the chromosome immigration method, it is very difficult to pick up a single cell into which chromosomes have immigrated.…”

mentioning

confidence: 94%

Two‐step chromosomal control of tumorigenicity of chinese hamster cells in nude mice

Ebina

Koi

Ishida

1977

Intl Journal of Cancer

View full text Add to dashboard Cite

A simple method for microinjecting isolated chromosomes into a single living cell under an inverted microscope has been developed. Of the 368 injected cells, 85 were able to form a colony and could be cloned. Clones of Chinese hamster V79 cells microinjected with chromosomes isolated from murine D56 cells [V79 (D56) cells] were tested for tumorigenicity in immunodeficient nude mice and for colony-forming ability in soft agar. Untreated recipient V79 cells were highly tumorigenic and had a high colony-forming ability in soft agar. In contrast, two out of the 21 microinjected clones tested were non-tumorigenic in nude mice and had only weak colony-forming ability in soft agar. The chromosome banding pattern was analyzed in microinjected clones and tumors derived from cells of these clones. In cells of the two non-tumorigenic clones, a telocentric chhromosome 1 (t1) was specifically involved in translocations with other chromosomes or chromosome fragments. In all tumor cells obtained from nude mice, a supernumerary piece or a whole biarmed chromosome 14 (b14) was specifically found. The results suggest that the t1 chromosome bears the gene which controls in vitro transformation and that the additional genetic change, i.e. the extra piece of b14 chromosome, was required for tumor formation in vivo.

show abstract

Transfer of the human gene for hypoxanthine-guanine phosphoribosyltransferase via isolated human metaphase chromosomes into mouse L-cells.

Cited by 116 publications

References 26 publications

Molecular and physical arrangements of human DNA in HRAS1-selected, chromosome-mediated transfectants.

Molecular and physical arrangements of human DNA in HRAS1-selected, chromosome-mediated transfectants.

Monochromosomal Hybrids and Chromosome Transfer: A Functional Approach for Gene Identification

Two‐step chromosomal control of tumorigenicity of chinese hamster cells in nude mice

Contact Info

Product

Resources

About