DNA Metabarcoding Reveals Cryptic Diversity in Forest Soils on the Isolated Brazilian Trindade Island, South Atlantic

Câmara, Paulo Eduardo Aguiar Saraiva; Bones, Fábio Leal Viana; Lopes, Fabyano Álvares Cardoso; Oliveira, Fábio Soares de; Barreto, Cristine Chaves; Henriques, Diego Knop; Campos, Larissa; Carvalho‐Silva, Micheline; Convey, Peter; Rosa, Luiz Henrique

doi:10.1007/s00248-022-02018-4

Cited by 5 publications

(5 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Alves is a lichen species that is considered a putative endemic taxon for the island (Lucking et al, 2015). More recently, based on a metabarcoding approach, Câmara et al (2022) pointed out the occurrence of five phyla that were not recorded before and the occurrence of 247 species (113 identified at the species level). This study represents an increase of almost 10-fold in the number of species registered to occur in the Trindade.…”

Section: Discussionmentioning

confidence: 99%

“…Lodge, Vizzini, Ercole & Boertm. was recovered in a recent soil metabarcoding study conducted in Trindade (Câmara et al, 2022), and it is considered Endangered (Jordal, 2019). Despite G. vitellinus being described as an oceanic species, its current distribution is restricted to the coast of European countries, and metabarcoding studies are known to have a considerable degree of bias from DNA extraction to bioinformatic treatment (Nilsson et al, 2019), which can undermine taxonomic classification.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Perdida em Meio à Vastidão do Oceano Atlântico: Síntese da Tendência de Publicações, Diversidade Biológica e Conservação na Ilha da Trindade

et al. 2023

View full text Add to dashboard Cite

Trindade é uma ilha oceânica de origem vulcânica no Atlântico Sul. Embora tenhasofrido muitos impactos ambientais, a ilha abriga uma rica diversidade biológica, sendo habitatde várias espécies endêmicas. As pesquisas científicas nos últimos 300 anos geraram um grandevolume de literatura especializada. No entanto, não existe uma síntese científica sumarizando osprincipais achados das pesquisas no último século na ilha, o que dificulta o entendimento das lacunasdo conhecimento, bem como o reconhecimento da biodiversidade na ilha e como promover suaconservação. Essa revisão tem por objetivo apresentar uma síntese da pesquisa científica realizada naIlha da Trindade, com ênfase na área de ciências biológicas. Para tanto, foi realizada uma busca onlinecom palavras-chave no CrossRef, Google Scholar e NCBI, e pesquisa sobre a ameaça de extinçãoglobal na lista vermelha da União Internacional para a Conservação da Natureza. Foram recuperadas312 referências, principalmente de pesquisas da área de Ciências Biológicas, da Saúde e Agrárias. Maisde 1000 espécies divididas em cinco reinos têm ocorrência relatada na ilha, a maioria em ambientesoceânicos. Trinta e duas espécies que ocorrem em Trindade estão ameaçadas de extinção. Animais eplantas são os organismos mais estudados, enquanto a diversidade de microrganismos é a principallacuna de conhecimento no reconhecimento da biota da ilha. Dados sobre a diversidade biológica da ilha, conservação e tendências de publicação são apresentados e discutidos. Uma lista de todasas espécies relatadas na literatura recuperada e seu estado de conservação também é apresentada.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Perdida em Meio à Vastidão do Oceano Atlântico: Síntese da Tendência de Publicações, Diversidade Biológica e Conservação na Ilha da Trindade

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Geographic distribution follows Petz (2005), Thompson et al (2019), Guiry and Guiry (2021), and Tropicos (http://www.tropicos.org). We used the number of reads as a proxy for abundance following the approach used by Deiner et al (2017), Hering et al (2018), Câmara et al (2020, Câmara, de Menezes, et al, 2022; Câmara, Menezes, et al, 2022; Câmara, Bones, et al, 2022), and Rosa et al (2020, 2021). Sequences were submitted to GenBank and accession numbers are given in Appendix 1.…”

Section: Methodsmentioning

confidence: 99%

Investigating aerial diversity of non‐fungal eukaryotes across a 40° latitudinal transect using DNA metabarcoding

et al. 2023

View full text Add to dashboard Cite

The high number of bipolar and widespread bryophyte and microbial taxa in the Antarctic flora suggests the effective Long‐Distance Dispersal (LDD) of spores and other propagules from lower latitudes and even the Northern Hemisphere to Antarctica and the sub‐Antarctic regions. However, few studies have attempted to document the transfer mechanisms by which potential newcomers may arrive in Antarctica. Commonly suggested or assumed mechanisms include transport in air currents, adventitious transfer with migrating or vagrant birds, and with human assistance. In this study, we investigated the biodiversity present in the air along a 40° latitudinal transect, from the port city of Rio de Janeiro, Brazil (ca. 22°S) to Comandante Ferraz Station on King George Island (South Shetland Islands, ca. 62°S), maritime Antarctica, to shed light on the potential role of LDD in species distribution. Air samples were collected in October 2021 on the Brazilian polar support vessel Ary Rongel using air filters with membranes of 0.22 μm. Total DNA was extracted from the filters and the Internal Transcribed Spacer (ITS2) DNA sequence was used for metabarcoding. A total of 53 non‐fungal taxa representing three kingdoms (most abundantly Viridiplantae) and six phyla (most abundantly Bryophyta and Magnoliophyta) were assigned from the sequences found. Aerial biodiversity was greater closer to the coast and generally decreased with increasing latitude, although a small increase was apparent in the South Shetland Islands. The taxa assigned are generally present in coastal biomes, although a small proportion of the assignments represented taxa of more distant origin, supporting the occurrence of LDD in the air column.

show abstract

“…For instance, evolutionary biologists rely on large molecular sequence datasets to study speciation trends ( Schlegel 1991 , Adl et al 2019 ). Large datasets help resolve cryptic diversity, which is a challenge seen across the tree of life—animals ( Marchán et al 2018 , Li and Wiens 2023 ), plants ( Vieu et al 2023 , Windham et al 2023 ), fungi ( Koufopanou et al 1997 , Pringle et al 2005 ), bacteria ( Meyer et al 2023 ), protists ( Wakeman and Leander 2013 , Krienitz et al 2015 , Martin et al 2016 ), archaea ( Câmara et al 2023 ), and viruses ( Roux et al 2019 ). Specialists across a range of taxa can therefore use GINSA to collect more data for their phylogenetic (SSU sequence) and biogeographic (locality) analyses.…”

Section: Applications and Implementationmentioning

confidence: 99%

GINSA: an accumulator for paired locality and next-generation small ribosomal subunit sequence data

Odle,

Kahng,

Riewluang

et al. 2024

Bioinformatics

View full text Add to dashboard Cite

Motivation Motivated by the challenges of decentralized genetic data spread across multiple international organizations, GINSA leverages the Global Biodiversity Information Facility (GBIF) infrastructure to automatically retrieve and link small ribosomal subunit (SSU) sequences with locality information. Results Testing on taxa from major organism groups demonstrates broad applicability across taxonomic levels and dataset sizes. Availability GINSA is a freely accessible Python program under the MIT License and can be installed from PyPI via pip. Supplementary information Supplementary data are available at Bioinformatics. Project code available at https://github.com/ericodle/GINSA.

show abstract

DNA Metabarcoding Reveals Cryptic Diversity in Forest Soils on the Isolated Brazilian Trindade Island, South Atlantic

Cited by 5 publications

References 84 publications

Perdida em Meio à Vastidão do Oceano Atlântico: Síntese da Tendência de Publicações, Diversidade Biológica e Conservação na Ilha da Trindade

Perdida em Meio à Vastidão do Oceano Atlântico: Síntese da Tendência de Publicações, Diversidade Biológica e Conservação na Ilha da Trindade

Investigating aerial diversity of non‐fungal eukaryotes across a 40° latitudinal transect using DNA metabarcoding

GINSA: an accumulator for paired locality and next-generation small ribosomal subunit sequence data

Contact Info

Product

Resources

About