DNA Methylation and Cancer Diagnosis

Delpu, Yannick; Cordelier, Pierre; Cho, William C.; Torrisani, Jérôme

doi:10.3390/ijms140715029

Cited by 138 publications

(97 citation statements)

References 150 publications

(170 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…for the establishment of DNA methylation patterns during development [12][13]. In this study we demonstrated that DNMTI procaine inhibited DNMT1 gene expression after 24 h of treatment.…”

Section: Procaine Induced Epigenetic Changes Mediated By Histone H3 Mmentioning

confidence: 70%

“…DNMT1 is known as maintenance DNMT which is responsible for the exact copying of the DNA methylation pattern on the neo-synthesized strand during DNA replication. DNMT1 expression is tightly regulated during the cell cycle by several mechanisms and maximal expression occurs during S phase [12][13]. The de novo DNA methyltransferases DNMT3a and DNMT3b are responsible Fig.4.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Anti-leukemic activity of DNA methyltransferase inhibitor procaine targeted on human leukaemia cells

2016

View full text Add to dashboard Cite

Chromatin remodeling in DNA is fundamental to gene expression, DNA replication and repair processes. Methylation of promoter regions of tumor-suppressor genes and histone deacetylation leads to gene silencing and transcriptionally repressive chromatin. For the past few decades DNA methylation agents became very attractive as the targets for cancer therapy. The purpose of this work was to examine the effects of DNMT inhibitor procaine on growth inhibition, apoptosis and differentiation of human leukaemia cells. The changes in expression of genes, proteins and histone modifications caused by procaine were evaluated under different treatments. We demonstrated that procaine arrests growth of human leukaemia cells and in combination with all-trans retinoic acid (ATRA) induces cancer cell differentiation. Procaine causes reduction of expression of DNA methyltransferases as well. The treatment of human leukaemia cells with procaine increase the expression of molecules associated with differentiation (CD11b, E-cadherin, G-CSF) and apoptosis (PPARγ). Moreover, the examined DNMT inhibitor enhances certain gene transcription activation via chromatin remodelling – the changes in histone H3K4(Me)3 and H3K9Ac/S10P modifications were detected. Our results suggest, that DNMT inhibitor – procaine, can be used for further investigations on epigenetic differentiation therapy of leukaemia cells especially when used in combination with retinoic acid.

show abstract

“…for the establishment of DNA methylation patterns during development [12][13]. In this study we demonstrated that DNMTI procaine inhibited DNMT1 gene expression after 24 h of treatment.…”

Section: Procaine Induced Epigenetic Changes Mediated By Histone H3 Mmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Anti-leukemic activity of DNA methyltransferase inhibitor procaine targeted on human leukaemia cells

2016

View full text Add to dashboard Cite

show abstract

“…DNMT1 plays prominent and pertinent role for DNA methylation during DNA replication. DNMT1 over expression was reported in lung, colorectal, prostate, breast, gastric and oral cavity cancers (Delpu et al, 2013). It has been pointed out that DNMT inhibition might lead to reactivation of the silenced genes.…”

Section: Discussionmentioning

confidence: 99%

Emodin Efficacy on the Akt, Mapk, Erk and DNMT Expression Pattern During Dmba-Induced Oral Carcinoma in Golden Syrian Hamsters

Manimaran

Manoharan

Neelakandan

2016

Afr J Tradit Complement Altern Med

View full text Add to dashboard Cite

Background: The present study has evaluated the Emodin efficacy on the Akt, MAPK, ERK and DNMT expression pattern during 7,12-dimethylbenz[a]anthracene (DMBA)-induced oral carcinoma in golden Syrian hamsters, in order to explore its antitumor potential. Materials and methods: Oral tumors were developed in the buccal pouches of golden Syrian hamsters using the carcinogen, DMBA. Results: While the incidence of tumor formation was 100% in hamsters treated with DMBA alone, the tumor formation was not noticed in DMBA+ Emodin treated hamsters. Also, Emodin reduced the severity of precancerous pathological lesions such as dysplasia, in the hamsters treated with DMBA. Emodin administration corrected the abnormalities in the expression pattern of Akt, MAPK, ERK and DNMT in the buccal mucosa of hamsters treated with DMBA. Conclusions:The present study thus suggests that the tumor preventive potential of Emodin is partly related to its modulating effect on the Akt, MAPK, ERK and DNMT expression pattern, as these molecular markers have a pivotal role in the process of cell proliferation, inflammation, invasion, and apoptosis.

show abstract

“…Since aberrant expression of DNMT1 occurs in the majority of cancer types, DNA methylation-based biomarkers mediated by DNMT1 have been used as diagnostic tools, with modification typically occurring in the CpG-rich island of the gene promoter region (19)(20)(21). Therefore, it has been suggested that DNA methylation patterns may be used to distinguish cancer cells from normal cells (22).…”

Section: Discussionmentioning

confidence: 99%

Silencing DNA methyltransferase 1 leads to the activation of the esophageal suppressor gene p16 in vitro and in vivo

et al. 2017

View full text Add to dashboard Cite

Abstract. Previous studies have demonstrated that DNA methyltransferase 1 (DNMT1) is required for the maintenance of DNA methylation and epigenetic changes that may lead to the development of esophageal squamous cell carcinoma (ESCC). In order to investigate whether the silencing of DNMT1 protects tumor suppressor genes, including p16, and is able to be used as a potential therapy for human ESCC, short hairpin RNA targeting DNMT1 (shRNA-DNMT1) was synthesized and transfected into the human ESCC lines KYSE150 and KYSE410, which were then injected into the backs of nude mice prior to harvesting. Results from the reverse transcription-quantitative polymerase chain reaction (PCR) and western blotting demonstrated that p16 mRNA expression was increased in the shRNA-DNMT1-transfected ESCC cell lines in vitro and in vivo. Consistent with the immunohistochemistry results, p16 was expressed in tumor tissue from nude mice that had been transplanted with the modified human ESCC lines. It was also observed that p16 methylation was inhibited following transfection with shRNA-DNMT1 as detected using methylation-specific PCR analysis. The results of the present study suggest that silencing DNMT1 serves a protective role through the demethylation and subsequent activation of p16 in vitro and in vivo.

show abstract

DNA Methylation and Cancer Diagnosis

Cited by 138 publications

References 150 publications

Anti-leukemic activity of DNA methyltransferase inhibitor procaine targeted on human leukaemia cells

Anti-leukemic activity of DNA methyltransferase inhibitor procaine targeted on human leukaemia cells

Emodin Efficacy on the Akt, Mapk, Erk and DNMT Expression Pattern During Dmba-Induced Oral Carcinoma in Golden Syrian Hamsters

Silencing DNA methyltransferase 1 leads to the activation of the esophageal suppressor gene p16 in vitro and in vivo

Contact Info

Product

Resources

About