Emodin Efficacy on the Akt, Mapk, Erk and DNMT Expression Pattern During Dmba-Induced Oral Carcinoma in Golden Syrian Hamsters

Manimaran, Asokan; Manoharan, Shanmugam; Neelakandan, Mani

doi:10.21010/ajtcam.v13i6.27

Cited by 7 publications

(2 citation statements)

References 59 publications

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“…The MAPKs – ERK, JNK, and p38 – are a class of proline-directed serine/threonine kinases, which are involved in the cell cycle, proliferation, and apoptosis ( Seger and Krebs, 1995 ; Rauch et al, 2016 ). Among these, the ERK cascade is stimulated by growth factors produced in prostatic cells ( Manimaran et al, 2016 ). It has been also reported that activated ERK1/2 signaling increase cancer cell survival and chemoresistance ( Tran et al, 2001 ; Llorens et al, 2004 ).…”

Section: Discussionmentioning

confidence: 99%

Berberine Improves Benign Prostatic Hyperplasia via Suppression of 5 Alpha Reductase and Extracellular Signal-Regulated Kinase in Vivo and in Vitro

et al. 2018

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Benign prostate hyperplasia (BPH) is a common disease in elderly men, characterized by proliferated prostate and urinary tract symptoms. The hormonal cascade starting by the action of 5-alpha-reductase (5AR) is known to be one of the pathways responsible for the pathogenesis of BPH. Present investigation evaluated the capacity of berberine (BBR), a nature-derived compound abundant in Coptis japonica, in testosterone-induced BPH rats. Experimental BPH was induced by inguinal injection with testosterone propionate (TP) for 4 weeks. BBR or finasteride, a 5AR inhibitor as positive control, was treated for 4 weeks during BPH. BPH induced by TP evoked weight gaining and histological changes of prostate and BBR treatment improved all the detrimental effects not only weight reduction and histological changes but also suppression of prostate-specific antigen (PSA), which is elevated during BPH. Additionally, BBR suppressed TP-associated increase of 5AR, androgen receptor (AR) and steroid coactivator-1 (SRC-1), the key factors in the pathogenesis of BPH. To evaluate the underlying molecular mechanisms responsible for beneficial effects of BBR, we investigated whether these effects were associated with the mitogen-activated protein kinase pathway. BPH induced by TP showed increased phosphorylation of extracellular signal-regulated kinase (ERK), whereas this was suppressed by BBR treatment. On the other hand, c-jun-N-terminal kinase (JNK) and p38 mitogen-activated protein kinase was not changed in BPH rats. In in vitro study using RWPE-1 cells, a human prostate epithelial cell line. TP increased cell proliferation and BPH-related key factors such as PSA, AR, and 5AR in RWPE-1 cells, and those factors were significantly decreased in the presence of BBR. Furthermore, these proliferative effects in RWPE-1cells were attenuated by treatment with U0126, an ERK inhibitor, confirming BBR can relieve overgrowth of prostate via ERK-dependent signaling. The cotreatment of U0126 and BBR did not affect the change of 5AR nor proliferation compared with U0126 alone, suggesting that the effect of BBR was dependent on the action of ERK. In conclusion, this study shows that BBR can be used as a therapeutic agent for BPH by controlling hyperplasia of prostate through suppression of ERK mechanism.

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Section: Discussionmentioning

confidence: 99%

Berberine Improves Benign Prostatic Hyperplasia via Suppression of 5 Alpha Reductase and Extracellular Signal-Regulated Kinase in Vivo and in Vitro

et al. 2018

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“…The MAPKs play a critical role in the regulation of cell growth, differentiation, and in the control of cellular responses to cytokines and stressor. It also was investigated that phosphorylation of Akt serine in U87MG glioblastoma cells treated at short times treatment (2 and 4 hr) with AE (20 and 40 μM) there is a decrease of phosphorylation that could be responsible of blocking of proliferative signals . We investigate the pro‐apoptotic AE effect that is already visible at first microscopic observation of the cells.…”

Section: Discussionmentioning

confidence: 99%

Effects of aloe emodin on U87MG glioblastoma cell growth: In vitro and in vivo study

Arcella

Oliva

Staffieri

et al. 2018

Environmental Toxicology

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Glioblastoma, the most aggressive and malignant form of glioma, appears to be resistant to various chemotherapeutic agents. Hence other approaches have been investigated to target more pathways involved in glioblastoma development and progression. Here we investigate the anticancer effect of Aloe-Emodin (AE), an anthraquinone compound presents in the leaves of Aloe arborescens, on human glioblastoma cell line U87MG. U87MG were treated with various concentrations of AE (20 and 40 μM) for different times (24, 48, and 72 hr). Cell growth was monitored by daily cell count after treatments. Growth analysis showed that AE significantly decrease proliferation of U87MG in a time and dose dependent manner. FACS analysis demonstrates a block of cell cycle in S and G2/M phase. AE probably induced also apoptosis by releasing of apoptosis-inducing factor: PARP and Lamin activation leading to nuclear shrinkage. In addition, exposure of U87MG to AE reduced pAKT phosphorylation. AE inhibition of U87MG growth is a result of more mechanism together. Here we report that AE has a specific growth inhibition on U87MG also in in vivo. The growth of U87MG, subcutaneously injected in nude mice with severe combined immunodeficiency, is inhibited without any appreciable toxic effects on the animals after AE treatment. AE might represent a conceptually new lead antitumor adjuvant drug.

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Natural bioactive compounds targeting DNA methyltransferase enzymes in cancer: Mechanisms insights and efficiencies

Aanniz,

Bouyahya,

Balahbib

et al. 2024

Chemico-Biological Interactions

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Emodin Efficacy on the Akt, Mapk, Erk and DNMT Expression Pattern During Dmba-Induced Oral Carcinoma in Golden Syrian Hamsters

Cited by 7 publications

References 59 publications

Berberine Improves Benign Prostatic Hyperplasia via Suppression of 5 Alpha Reductase and Extracellular Signal-Regulated Kinase in Vivo and in Vitro

Berberine Improves Benign Prostatic Hyperplasia via Suppression of 5 Alpha Reductase and Extracellular Signal-Regulated Kinase in Vivo and in Vitro

Effects of aloe emodin on U87MG glioblastoma cell growth: In vitro and in vivo study

Natural bioactive compounds targeting DNA methyltransferase enzymes in cancer: Mechanisms insights and efficiencies

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