DNA methylation changes in triticale due to in vitro culture plant regeneration and consecutive reproduction

Machczyńska, Joanna; Orłowska, Renata; Mańkowski, Dariusz R.; Zimny, J.; Bednarek, Piotr Tomasz

doi:10.1007/s11240-014-0533-1

Cited by 55 publications

(57 citation statements)

References 52 publications

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“…In some species, they may reach up to several percentages of detected changes (Bednarek et al 2007;Fiuk et al 2010;Machczyńska et al 2014a). The in vitro tissue culture-induced DNA methylation pattern alterations were described, for example, in maize (Brown 1989) and rice (Müller et al 1990;Zheng et al 1987).…”

Section: Dna Methylation Pattern Alterationsmentioning

confidence: 99%

Plant tissue culture environment as a switch-key of (epi)genetic changes

Bednarek

Orłowska

2019

Plant Cell Tiss Organ Cult

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103

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The in vitro tissue cultures are, beyond all difficulties, an essential tool in basic research as well as in commercial applications. Numerous works devoted to plant tissue cultures proved how important this part of the plant science is. Despite half a century of research on the issue of obtaining plants in in vitro cultures, many aspects remain unknown. The path associated with the reprogramming of explants in the fully functioning regenerants includes a series of processes that may result in the appearance of morphological, physiological, biochemical or, finally, genetic and epigenetic changes. All these changes occurring at the tissue culture stage and appearing in regenerants as tissue culture-induced variation and then inherited by generative progeny as somaclonal variation may be the result of oxidative stress, which works at the step of explant preparation, and in tissue culture as a result of nutrient components and environmental factors. In this review, we describe the current status of understanding the genetic and epigenetic changes that occur during tissue culture. Key message Variation appeared in regenerated plants as well as variation inherited by generative progeny of regenerants can may many, positive or negative impact, of gained plant materials. This review focused on factors that triggered this phenomenon with underlying oxidative stress.

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Section: Dna Methylation Pattern Alterationsmentioning

confidence: 99%

Plant tissue culture environment as a switch-key of (epi)genetic changes

Bednarek

Orłowska

2019

Plant Cell Tiss Organ Cult

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103

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“…Triticale (× Triticosecale Wittmack) is a recognized artificial amphiploid cereal that has been remarkably developed by classical breeding and almost 3 × 10 6 ha of triticale is cultivated today in the world (Lelley and Gimbel, 1989;Machzynska et al, 2014; http://www.fao. org/faostat/en/).…”

Section: Introductionmentioning

confidence: 99%

Response of five triticale genotypes to salt stress in in vitro culture

Bezirganoğlu¹

2017

Turk J Agric For

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Different techniques have been suggested to protect plants from environmental stress. Therefore, new techniques should be developed to produce salt-resistant genotypes. Selection and improvement of desirable genotypes for this objective require suitable screening methods. Tissue culture can help in the efforts to produce new cultivars against environmental stress factors. In addition, in vitro culture studies permit relatively faster responses, shorter generation time, and regular environmental conditions as compared to classical breeding methods (Zhao et al., 2009;Elmaghrabi et al., 2013). A high level of salt in soil or in tissue culture may lead to numerous genetic and biochemical changes, causing problems such as limitation in mineral nutrient uptake, nutritional imbalance, mineral deficiency, osmotic stress, ion toxicity, and oxidative stress (Rozema and Flower, 2008;Rahnama et al., 2010;James et al., 2011). It has been reported that oxidative and osmotic stresses affect the cellular membrane integrity, enzyme activity, DNA, and chlorophyll content (Lokhande et al., 2010), which inhibits the functioning of most plant species.Proline is the most common metabolite that accumulates in response to salinity stress, shown to serve as a main osmotic regulator, and was reported in various plant species (Koca et al., 2007). The role of proline accumulation in plants' osmotic regulation is still unclear (Koskeroglu and Tuna, 2010). Soluble sugar is known to commonly accumulate in higher plants against salinity stress, which could play a role in osmotic protection (Khedr, 2003). Salt stress usually inhibits the plant growth. When plants are exposed to different abiotic stresses, some reactive oxygen species (ROS) removing enzymes such as superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), and catalase (CAT) are produced (Li and Staden, 1998). The antioxidant enzyme activity is positively associated with salt tolerance in plants (Lokhande et al., 2011). Many researchers have indicated that the salt resistance mechanism is activated during the entire plant stage and this has been tested in both in vitro and ex vitro situations (Watanabe et al., 2000; Troncoso et al., 2002). A close relationship between physiological changes and callus cultures by salt stress has been previously reported in different genotypes (Piwowarczyk et al., 2016).

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“…Environmental conditions can induce changes to plant methylomes [13, 14]. In vitro culture of plant tissues has been reported to induced epigenetic somaclonal variation for multiple crop species including garlic [15], cassava [6], pineapple [16], cotton [17], cocoa [7], and other crops [5, 18]. However, few studies have evaluated the extent of DNA methylation changes during meristem propagation of sweetpotato.…”

Section: Introductionmentioning

confidence: 99%

Common garden experiment reveals altered nutritional values and DNA methylation profiles in micropropagated three elite Ghanaian sweet potato genotypes

Akomeah

Quain

Ramesh

et al. 2018

Preprint

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18 Micronutrient deficiency is the cause of multiple diseases in developing countries. Staple crop 19 biofortification is an efficient means to combat such deficiencies in the diets of local consumers. 20 Biofortified lines of sweet potato (Ipomoea batata L. Lam) with enhanced beta-carotene content 21 have been developed in Ghana to alleviate Vitamin A Deficiency. These genotypes are propagated 22 using meristem micropropagation to ensure the generation of virus-free propagules. In vitro culture 23 exposes micropropagated plants to conditions that can lead to the accumulation of somaclonal 2 24 variation with the potential to generate unwanted aberrant phenotypes. However, the effect of 25 micropropagation induced somaclonal variation on the production of key nutrients by field-grown 26 plants has not been previously studied. Here we assessed the extent of in vitro culture induced 27 somaclonal variation, at a phenotypic, compositional and genetic/epigenetic level, by comparing 28 field-maintained and micropropagated lines of three elite Ghanaian sweet potato genotypes grown 29 in a common garden. Although micropropagated plants presented no observable morphological 30 abnormalities compared to field maintained lines, they presented significantly lower levels of iron, 31 total protein, zinc, and glucose. Methylation Sensitive Amplification Polymorphism analysis 32 showed a high level of in vitro culture induced molecular variation in micropropagated plants.33 Epigenetic, rather than genetic variation, accounts for most of the observed molecular variability.34 Taken collectively, our results highlight the importance of ensuring the clonal fidelity of the 35 micropropagated biofortified lines in order to reduce potential losses in the nutritional value prior 36 to their commercial release.3 37 Introduction 38 Sweet potato (Ipomoea batatas L. Lam), is a drought tolerant, low input, and high yielding crop, 39 which produces more nutrients and has higher edible energy than most staples such as rice, 40 cassava, wheat, and sorghum [1]. As a predominantly vegetatively propagated crop, virus 41 accumulation in vegetative propagules (i.e. vine cuttings and tubers) can cause devastating loss in 42 yield and poor root quality in subsequent cultivation [2]. Micropropagation techniques, such as 43 meristem or nodal tip culture, coupled with thermotherapy or cryotherapy, are currently the 44 principal plant tissue culture (PTC) methods for producing healthy (pathogen-tested/disease-free) 45 clones of planting materials [3]. However, the generation of true-to-type material through in vitro 46 propagation can be challenging due to somaclonal variation [4]. 47 48 Somaclonal variation refers to changes that can be induced during in vitro tissue culture and have 49 been reported in all in vitro systems [5-8]. Such changes can be genetic and/or epigenetic in nature. 50 Epigenetic modifications are heritable changes that can affect the phenotype without changes to 51 the DNA sequence [9]. These are mediated, among other mechanisms, b...

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DNA methylation changes in triticale due to in vitro culture plant regeneration and consecutive reproduction

Cited by 55 publications

References 52 publications

Plant tissue culture environment as a switch-key of (epi)genetic changes

Plant tissue culture environment as a switch-key of (epi)genetic changes

Response of five triticale genotypes to salt stress in in vitro culture

Common garden experiment reveals altered nutritional values and DNA methylation profiles in micropropagated three elite Ghanaian sweet potato genotypes

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