DNA Methylation of the ABO Promoter Underlies Loss of ABO Allelic Expression in a Significant Proportion of Leukemic Patients

Bianco‐Miotto, Tina; Hussey, Damian J.; Day, Tanya K.; O’Keefe, Denise S.; Dobrovic, Alexander

doi:10.1371/journal.pone.0004788

Cited by 61 publications

(49 citation statements)

References 47 publications

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“…This is due to loss of heterozygosity, a promoter methylation, and increase of gene expression or neoexpression [65-67]. Hypoxic microenvironments change the expression of glycosyltransferase genes, such as fucosyltransferases (FUTs), N -acetylglucosaminyltransferases (GnTs), and sialyltransferases (STs) [68-70].…”

Section: Hypoxia Regulates Glycosyltransferases Glycosidases and Nucmentioning

confidence: 99%

Glycobiology Modifications in Intratumoral Hypoxia: The Breathless Side of Glycans Interaction

Silva-filho¹,

Sena²,

Lima

et al. 2017

Cell Physiol Biochem

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Post-translational and co-translational enzymatic addition of glycans (glycosylation) to proteins, lipids, and other carbohydrates, is a powerful regulator of the molecular machinery involved in cell cycle, adhesion, invasion, and signal transduction, and is usually seen in both in vivo and in vitro cancer models. Glycosyltransferases can alter the glycosylation pattern of normal cells, subsequently leading to the establishment and progression of several diseases, including cancer. Furthermore, a growing amount of research has shown that different oxygen tensions, mainly hypoxia, leads to a markedly altered glycosylation, resulting in altered glycan-receptor interactions. Alteration of intracellular glucose metabolism, from aerobic cellular respiration to anaerobic glycolysis, inhibition of integrin 3α1β translocation to the plasma membrane, decreased 1,2-fucosylation of cell-surface glycans, and galectin overexpression are some consequences of the hypoxic tumor microenvironment. Additionally, increased expression of gangliosides carrying N-glycolyl sialic acid can also be significantly affected by hypoxia. For all these reasons, it is possible to realize that hypoxia strongly alters glycobiologic events within tumors, leading to changes in their behavior. This review aims to analyze the complexity and importance of glycoconjugates and their molecular interaction network in the hypoxic context of many solid tumors.

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Section: Hypoxia Regulates Glycosyltransferases Glycosidases and Nucmentioning

confidence: 99%

Glycobiology Modifications in Intratumoral Hypoxia: The Breathless Side of Glycans Interaction

Silva-filho¹,

Sena²,

Lima

et al. 2017

Cell Physiol Biochem

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“…Le système de groupes sanguins ABO est le principal type des groupes sanguins classés en quatre phénotypes (A, B, O et AB) [4] [5] [6] [11] [12] selon la présence ou l'absence des antigène et anticorps spécifiques sur la membrane des hématies ou dans le plasma [7] [9] [10]. Ces antigènes (localisés sur le chromosome 9) sont de nature glucidique: D-galactose pour l'antigène B et N-acétylgalactosamine pour l'antigène A [1] [10] [13] [14]. Génétiquement le A et B sont des allèles Codominant et le O est récessif [13].…”

Section: Introductionunclassified

Frequency of Erythrocyte Phenotypes in Blood Group Systems ABO and Rhesus at Moba, Province of Tanganyika, Democratic Republic of Congo

Kabemba¹,

Kabobo²,

Mukena³

et al. 2017

OALib

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The determination of blood groups appears to be very useful in transfusion medicine, genetics, forensic medicine, organ transplantation and maternal alloimmunization. In Moba, blood transfusion is indicated in several anemias of the children. The present study aimed to determine the frequency of ABO and Rhesus blood groups in our environment. This is a descriptive cross-sectional study. Blood donors registered at the Moba General Reference Hospital for the period 2015 to 2016 were selected for this study (n = 2292). The Beth-Vincent test was used to determine blood group phenotypes from the monoclonal test sera. Blood groups O (60.5%) and AB (2.5%) were respectively the most frequent and the least encountered. In our series of studies, the numerical frequency order of the phenotypes of the ABO and Rhesus (D) blood groups included in order of importance: O + (n = 1364 or 59.5%); A + (n = 488 or 21.3%); B + (n = 348 or 15.2%); AB + (n = 55 or 2.4%); O − (n = 22 or 1.0%); A − (n = 7 or 0.3%); B − (n = 5 or 0.2%); and AB − (n = 3 or 0.1%). This observed difference between the different ABO and Rhesus groups is significant. Sex does not significantly affect the type of blood group. The distribution of ABO and rhesus groups follows the same Negroid distribution as in many countries. The results should be capitalized for proper priority management of the blood to be stored in the blood bank.

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“…This process is significant during the development, differentiation, and maturation of normal cells [6]. ABH antigens are deleted or reduced in various cancers including myeloid malignancies [7], leukemia [8], oral cancer [9], and bladder cancer [10]. The A-transferase gene promoter region contains CpG-rich sites whose methylation status correlates well with gene expression; treatment with 5-aza-dC results in the appearance of A-transferase gene and A-antigen expression [11].…”

Section: Introductionmentioning

confidence: 99%

“…Loss of H antigen in myeloid malignancies and leukemia [7, 8] and loss of α 1,2-fucosyltransferase (FUT) activities in gastric cancer [15] are common, although the mechanism remains to be elucidated. FUT1 (H enzyme) and FUT2 (Se enzyme) genes are cloned and responsible for synthesis of α 1,2-fucosylation on both core structures of type 1/2, Gal β 1,3/4GlcNAc-R, producing H antigen and Lewis B/Y, Fuc α 1,2Gal β 1,3/4(Fuc α 1,4/3)GlcNAc-R [16, 17].…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells

Taniuchi

Higai

Tanaka

et al. 2013

The Scientific World Journal

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The α1,2-fucosyltransferase I (FUT1) enzyme is important for the biosynthesis of H antigens, Lewis B, and Lewis Y. In this study, we clarified the transcriptional regulation of FUT1 in the DLD-1 colon cancer cell line, which has high expression of Lewis B and Lewis Y antigens, expresses the FUT1 gene, and shows α1,2-fucosyltransferase (FUT) activity. 5′-rapid amplification of cDNA ends revealed a FUT1 transcriptional start site −10 nucleotides upstream of the site registered at NM_000148 in the DataBase of Human Transcription Start Sites (DBTSS). Using the dual luciferase assay, FUT1 gene expression was shown to be regulated at the region −91 to −81 nt to the transcriptional start site, which contains the Elk-1 binding site. Site-directed mutagenesis of this region revealed the Elk-1 binding site to be essential for FUT1 transcription. Furthermore, transfection of the dominant negative Elk-1 gene, and the chromatin immunoprecipitation (CHIp) assay, supported Elk-1-dependent transcriptional regulation of FUT1 gene expression in DLD-1 cells. These results suggest that a defined region in the 5′-flanking region of FUT1 is critical for FUT1 transcription and that constitutive gene expression of FUT1 is regulated by Elk-1 in DLD-1 cells.

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DNA Methylation of the ABO Promoter Underlies Loss of ABO Allelic Expression in a Significant Proportion of Leukemic Patients

Cited by 61 publications

References 47 publications

Glycobiology Modifications in Intratumoral Hypoxia: The Breathless Side of Glycans Interaction

Glycobiology Modifications in Intratumoral Hypoxia: The Breathless Side of Glycans Interaction

Frequency of Erythrocyte Phenotypes in Blood Group Systems ABO and Rhesus at Moba, Province of Tanganyika, Democratic Republic of Congo

Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells

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