DNA methylation regulated by ascorbic acids in yak preimplantation embryo helps to improve blastocyst quality

Li, Qin; He, Honghong; Hu, Xuequan; Zhao, Tian; Jiang, Jia-Ying; Cui, Yan; Xu, Gezhi; Wang, Yunlong; He, Junlin; Fan, Jinjiang; Yu, Sijiu

doi:10.1002/mrd.23230

Cited by 8 publications

(8 citation statements)

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“…This is a problem worthy of in‐depth study. In addition, studies have found that high concentrations of antioxidants (ascorbic acid, AA) can increase apoptosis during embryonic development (Li et al, 2019), and we believe that PB1 may also have this situation. We will further study the toxic effects of high concentrations of PB1 and the mechanism of promoting apoptosis.…”

Section: Discussionmentioning

confidence: 67%

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Procyanidin B1 promotes in vitro maturation of pig oocytes by reducing oxidative stress

Jin

Hao

Huang

et al. 2020

Molecular Reproduction Devel

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Oxidative stress negatively affects the in vitro maturation (IVM) of oocytes. Procyanidin B1 (PB1) is a natural polyphenolic compound that has antioxidant properties. In this study, we investigated the effect of PB1 supplementation during IVM of porcine oocytes. Treatment with 100 μM PB1 significantly increased the MII oocytes rate (p <0.05), the parthenogenetic (PA) blastocyst rate (p <0.01) and the total cell number in the PA blastocyst (p < 0.01) which were cultured in regular in vitro culture (IVC) medium. The PA blastocyst rate of regular MII oocytes activated and cultured in IVC medium supplemented with 100 and 150 μM PB1 significantly increased compared with control (p < 0.01 and p < 0.05). We also evaluated the reactive oxygen species (ROS) levels, mitochondrial membrane potential (Δψm) levels, glutathione (GSH) levels, and apoptotic levels in MII oocytes and cumulus cells following 100 μM PB1 treatment. The results showed that the PB1 supplementation decreased ROS production and apoptotic levels. In addition, PB1 was found to increase Δψm levels and GSH levels. In conclusion, PB1 inhibited apoptosis of oocytes and cumulus cells by reducing oxidative stress. Moreover, PB1 improved the quality of oocytes and promoted PA embryo development. Taken together, our results suggest that PB1 is a promising antioxidant additive for IVM of oocytes.

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Section: Discussionmentioning

confidence: 67%

“…PB1, procyanidin B1; qRT-PCR, quantitative reverse transcription polymerase chain reaction. *p < 0.05, **p < 0.01 and ***p < 0.001 development (Li et al, 2019), and we believe that PB1 may also have this situation. We will further study the toxic effects of high concentrations of PB1 and the mechanism of promoting apoptosis.…”

Section: Discussionmentioning

confidence: 71%

Procyanidin B1 promotes in vitro maturation of pig oocytes by reducing oxidative stress

Jin

Hao

Huang

et al. 2020

Molecular Reproduction Devel

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“…Then, the COCs were washed 3 times and counterstained with DAPI (10 µg/ml) for 5 min in the dark. The COCs were again washed three times and mounted with coverslip and photographed using fluorescence microscope (Olympus) (Li et al., 2019). Five replicates were performed with 8 COCs per each replicate.…”

Section: Methodsmentioning

confidence: 99%

Low oxygen concentration improves yak oocyte maturation and inhibits apoptosis through HIF‐1 and VEGF

Zhang

et al. 2022

Reprod Domestic Animals

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The gas-phase environment of in vitro culture system plays an important role in the development of oocytes, and oxygen concentration is one of the important factors. In the present study, we aimed to explore the effect of different oxygen concentrations (20%, 10%, 5% or 1% O 2 ) in yak oocyte maturation and to detect the expression of hypoxia-inducible factor 1α (HIF-1α), vascular endothelial growth factor (VEGF) and cell apoptosis in yak COCs. First, the maturation rate of oocytes, cleavage rate and blastocysts rate following parthenogenetic activation in the group with 5% oxygen concentration were significantly higher (p < .05) than the other groups. Then, TUNEL analysis showed that the 5% oxygen concentration group significantly inhibited apoptosis of cumulus-oocyte complexes (COCs) compared to the other group, and the transcription and protein levels of pro-apoptotic factor Bax, HIF-1α and VEGF in yak COCs significantly reduced, while anti-apoptotic factor Bcl-2 significantly increased.Furthermore, immunohistochemical staining results indicated that HIF-1α protein was mainly located in theca follicle interna, mural follicular stratum granulosum, corona radiata and ovarian stroma in the follicular ovarian tissue, while VEGF protein was mainly located in the granulosa and theca cell layers. In summary, our findings demonstrate that 5% oxygen concentration may promote maturation and inhibit apoptosis of oocytes through HIF-1α-mediated VEGF expression.

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“…The immature cumulus-oocyte complexes were put in maturation media and cultivated in a 38°C, 5% CO 2 humidified incubator for 24 h. The maturation fluid consisted of TCM-199 (Gibco, Grand Island, NY, USA), 10% FBS, 10 μg/mL follicle-stimulating hormone (FSH), 10 μg/mL Luteinizing hormone (LH) and 1 μg/mL β-Estradiol (E 2 ) ( 28 ). The final concentrations of LIF (LIF1050, Sigma Aldrich, USA) were 0 ng/mL, 25 ng/mL, 50 ng/mL and 100 ng/mL by adding leukemia inhibitory factor to the maturation medium.…”

Section: Methodsmentioning

confidence: 99%

Leukemia inhibitory factor enhances the development and subsequent blastocysts quality of yak oocytes in vitro

Zhao

Ding

et al. 2022

Front. Vet. Sci.

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Leukemia inhibitory factor (LIF) is a multipotent cytokine of the IL-6 family which plays a critical role in the maturation and development of oocytes. This study evaluated the influence of LIF on the maturation and development ability of yak oocytes, and the quality of subsequent blastocysts under in vitro culture settings. Different concentrations of LIF (0, 25, 50, and 100 ng/mL) were added during the in vitro culture of oocytes to detect the maturation rate of oocytes, levels of mitochondria, reactive oxygen species (ROS), actin, and apoptosis in oocytes, mRNA transcription levels of apoptosis and antioxidant-related genes in oocytes, and total cell number and apoptosis levels in subsequent blastocysts. The findings revealed that 50 ng/mL LIF could significantly increase the maturation rate (p < 0.01), levels of mitochondria (p < 0.01) and actin (p < 0.01), and mRNA transcription levels of anti-apoptotic and antioxidant-related genes in yak oocytes. Also, 50 ng/mL LIF could significantly lower the generation of ROS (p < 0.01) and apoptosis levels of oocytes (p < 0.01). In addition, blastocysts formed from 50 ng/mL LIF-treated oocytes showed significantly larger total cell numbers (p < 0.01) and lower apoptosis rates (p < 0.01) than the control group. In conclusion, the addition of LIF during the in vitro maturation of yak oocytes improved the quality and the competence of maturation and development in oocytes, as well as the quality of subsequent blastocysts. The result of this study provided some insights into the role and function of LIF in vitro yak oocytes maturation, as well as provided fundamental knowledge for assisted reproductive technologies in the yak.

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DNA methylation regulated by ascorbic acids in yak preimplantation embryo helps to improve blastocyst quality

Cited by 8 publications

References 50 publications

Procyanidin B1 promotes in vitro maturation of pig oocytes by reducing oxidative stress

Procyanidin B1 promotes in vitro maturation of pig oocytes by reducing oxidative stress

Low oxygen concentration improves yak oocyte maturation and inhibits apoptosis through HIF‐1 and VEGF

Leukemia inhibitory factor enhances the development and subsequent blastocysts quality of yak oocytes in vitro

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