DNA methyltransferase mediates dose-dependent stimulation of neural stem cell proliferation by folate

Li, Wen; Yu, Min; Luo, Suhui; Liu, Huan; Gao, Yuxia; Wilson, John X.; Huang, Guowei

doi:10.1016/j.jnutbio.2012.11.001

Cited by 32 publications

(30 citation statements)

References 27 publications

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“…Folate may confer neuroprotection because it acts as a cofactor in the elimination of HCY by the latter's remethylation [3]. Another potentially neuroprotective effect is that folate acts through DNMT to modulate DNA methylation and stimulate neural stem cell proliferation [28,29].…”

Section: Discussionmentioning

confidence: 99%

Folic acid administration inhibits amyloid β-peptide accumulation in APP/PS1 transgenic mice

Liu

et al. 2015

The Journal of Nutritional Biochemistry

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Section: Discussionmentioning

confidence: 99%

Folic acid administration inhibits amyloid β-peptide accumulation in APP/PS1 transgenic mice

Liu

et al. 2015

The Journal of Nutritional Biochemistry

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“…Proliferating NSCs were identified as proliferating cell nuclear antigen (PCNA)/sex determining region Y box 2 (SOX2)-double-positive cells. SOX2 is essential to maintain self-renewal of undifferentiated stem cells, and it is commonly used to identify NSCs (Suh et al 2007;Li et al 2013). The brain tissue was fixed with 4% paraformaldehyde for 24 h at 4°C, then dehydrated with gradient alcohol, embedded in paraffin, and cut coronally into 4 μm-thick sections.…”

Section: Immunofluorescence Analysismentioning

confidence: 99%

“…Primary NSC culture protocols have been described previously (Li et al 2013). Briefly, hippocampus was dissected from PND0 offspring and plated at 1 × 10 6 cells/mL in T25 culture flasks (Corning, NY, USA), then cultured in a 5% CO 2 contained humidified atmosphere at 37°C.…”

Section: Nsc Culturementioning

confidence: 99%

“…Supplementation with folic acid during the periconceptional period (from 4 to 12 weeks preconception to the end of first trimester of pregnancy) decreases neural tube defects incidence (Chen et al 2012;Gomes et al 2016) and improves cognitive performance in offspring (McGarel et al 2015). In vitro studies have shown that folic acid stimulates cultured neural stem cells (NSCs) to proliferate (Zhang et al 2009;Li et al 2013;Yu et al 2014) and differentiate into neurons (Luo et al 2013). However, these studies in vitro may not fully represent central nervous system development in vivo, and we are not aware of any detailed in vivo experiments giving direct evidence of the effect of maternal folic acid supplementation during pregnancy on neuronal development in offspring.…”

Section: Introductionmentioning

confidence: 99%

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Maternal Folic Acid Supplementation During Pregnancy Promotes Neurogenesis and Synaptogenesis in Neonatal Rat Offspring

Wang

et al. 2018

Cerebral Cortex

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Maternal folic acid supplementation during pregnancy is associated with improved cognitive performances in offspring. However, the effect of supplementation on offspring's neurogenesis and synaptogenesis is unknown, and whether supplementation should be continued throughout pregnancy is controversial. In present study, 3 groups of female rats were fed a folate-normal diet, folate-deficient diet, or folate-supplemented diet from 1 week before mating until the end of pregnancy. A fourth group fed folate-normal diet from 1 week before mating until mating, then fed folate-supplemented diet for 10 consecutive days, then fed folate-normal diet until the end of pregnancy. Offspring were sacrificed on postnatal day 0 for measurement of neurogenesis and synaptogenesis by immunofluorescence and western blot. Additionally neural stem cells (NSCs) were cultured from offspring's hippocampus for immunocytochemical measurement of their rates of proliferation and neuronal differentiation. The results demonstrated that maternal folic acid supplementation stimulated hippocampal neurogenesis by increasing proliferation and neuronal differentiation of NSCs, and also enhanced synaptogenesis in cerebral cortex of neonatal offspring. Hippocampal neurogenesis was stimulated more when supplementation was continued throughout pregnancy instead of being limited to the periconceptional period. In conclusion, maternal folic acid supplementation, especially if continued throughout pregnancy, improves neurogenesis and synaptogenesis in neonatal offspring.

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“…Folate deficiency suppressed the proliferation of adult hippo-campal NSPCs in vivo and of embryonic NSPCs in vitro [87]. NSPCs respond to folate with altered Notch signaling, increased cell proliferation [88, 89] and increased neuronal differentiation by enhancing the activity of DNA methyl-transferases (DNMTs) [90, 91]. These reports suggest that one-carbon metabolism may play a critical role in NSPC fate as DNMT maintains progenitor function in self-renewing somatic tissue [92].…”

Section: Metabolic Pathways In Neural Stem Cellsmentioning

confidence: 99%

Metabolic circuits in neural stem cells

Kim

Rhee

Paik

2014

Cell. Mol. Life Sci.

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Metabolic activity indicative of cellular demand is emerging as a key player in cell fate decision. Numerous studies have demonstrated that diverse metabolic pathways have a critical role in the control of the proliferation, differentiation and quiescence of stem cells. The identification of neural stem/progenitor cells (NSPCs) and the characterization of their development and fate decision process have provided insight into the regenerative potential of the adult brain. As a result, the potential of NSPCs in cell replacement therapies for neurological diseases is rapidly growing. The aim of this review is to discuss the recent findings on the crosstalk among key regulators of NSPC development and the metabolic regulation crucial for the function and cell fate decisions of NSPCs. Fundamental understanding of the metabolic circuits in NSPCs may help to provide novel approaches for reactivating neurogenesis to treat degenerative brain conditions and cognitive decline.

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DNA methyltransferase mediates dose-dependent stimulation of neural stem cell proliferation by folate

Cited by 32 publications

References 27 publications

Folic acid administration inhibits amyloid β-peptide accumulation in APP/PS1 transgenic mice

Folic acid administration inhibits amyloid β-peptide accumulation in APP/PS1 transgenic mice

Maternal Folic Acid Supplementation During Pregnancy Promotes Neurogenesis and Synaptogenesis in Neonatal Rat Offspring

Metabolic circuits in neural stem cells

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