DNA-Modified Plasmonic Sensor for the Direct Detection of Virus Biomarkers from the Blood

Vázquez-Guardado, Abraham; Mehta, Freya; Jiménez, Beatriz; Biswas, Aritra; Ray, Keval; Baksh, Aliyah; Lee, Sang; Saraf, Nileshi; Seal, Sudipta; Chanda, Debashis

doi:10.1021/acs.nanolett.1c01609

Cited by 28 publications

(20 citation statements)

References 42 publications

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“…Plasmonics deals with surface plasmon resonance (SPR), which can dramatically increase the light confinement and nearfield enhancement at the nanoscale, are thus capable of boosting the analyte sensitivity [261]. Recently, Guardado et al reported the detection of NS1 protein biomarkers related to the Dengue virus developed on a plasmonic optofluidic device [268]. The schematic and principle of their methodology are depicted in Figure 5a.…”

Section: On-chip Optical Detection Of Single Moleculesmentioning

confidence: 99%

A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices

Rahman

Islam

et al. 2022

Micromachines

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Single-molecule techniques have shifted the paradigm of biological measurements from ensemble measurements to probing individual molecules and propelled a rapid revolution in related fields. Compared to ensemble measurements of biomolecules, single-molecule techniques provide a breadth of information with a high spatial and temporal resolution at the molecular level. Usually, optical and electrical methods are two commonly employed methods for probing single molecules, and some platforms even offer the integration of these two methods such as optofluidics. The recent spark in technological advancement and the tremendous leap in fabrication techniques, microfluidics, and integrated optofluidics are paving the way toward low cost, chip-scale, portable, and point-of-care diagnostic and single-molecule analysis tools. This review provides the fundamentals and overview of commonly employed single-molecule methods including optical methods, electrical methods, force-based methods, combinatorial integrated methods, etc. In most single-molecule experiments, the ability to manipulate and exercise precise control over individual molecules plays a vital role, which sometimes defines the capabilities and limits of the operation. This review discusses different manipulation techniques including sorting and trapping individual particles. An insight into the control of single molecules is provided that mainly discusses the recent development of electrical control over single molecules. Overall, this review is designed to provide the fundamentals and recent advancements in different single-molecule techniques and their applications, with a special focus on the detection, manipulation, and control of single molecules on chip-scale devices.

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Section: On-chip Optical Detection Of Single Moleculesmentioning

confidence: 99%

A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices

Rahman

Islam

et al. 2022

Micromachines

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“…13 For examples, DNA aptamers exhibit specific recognition on target molecules; 14 responsive sequences such as DNA i-motifs and G-duplexes show conformational transformation upon stimuli; 15 catalytic DNAzymes initiate cleavage toward target sequences. 16,17 Because of these unique properties and functions, DNA has been extensively applied in the field of bioanalysis, including bioseparation, 18 biosensing, 19 biological detection, 20 and imaging. 21 Supramolecular assembly of DNA refers to the blending of rationally designed DNA building blocks with other molecules and imparting structural and functional advantages both to the initial self-assembly process and to the final construct.…”

Section: Introductionmentioning

confidence: 99%

DNA Supramolecular Assembly on Micro/Nanointerfaces for Bioanalysis

Yao

Tang

et al. 2022

Acc. Chem. Res.

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Metrics & MoreArticle Recommendations CONSPECTUS: Facing increasing demand for precision medicine, materials chemistry systems for bioanalysis with accurate molecular design, controllable structure, and adjustable biological activity are required. As a genetic biomacromolecule, deoxyribonucleic acid (DNA) is created via precise, efficient, and mild processes in life systems and can in turn precisely regulate life activities. From the perspective of materials chemistry, DNA possesses the characteristics of sequence programmability and can be endowed with customized functions by the rational design of sequences. In recent years, DNA has been considered to be a potential biomaterial for analysis and has been applied in the fields of bioseparation, biosensing, and detection imaging. To further improve the precision of bioanalysis, the supramolecular assembly of DNA on micro/nanointerfaces is an effective strategy to concentrate functional DNA modules, and thus the functions of DNA molecules for bioanalysis can be enriched and enhanced. Moreover, the new modes of DNA supramolecular assembly on micro/nanointerfaces enable the integration of DNA with the introduced components, breaking the restriction of limited functions of DNA materials and achieving more precise regulation and manipulation in bioanalysis. In this Account, we summarize our recent work on DNA supramolecular assembly on micro/nanointerfaces for bioanalysis from two main aspects. In the first part, we describe DNA supramolecular assembly on the interfaces of microscale living cells. The synthesis strategy of DNA is based on rolling-circle amplification (RCA), which generates ultralong DNA strands according to circular DNA templates. The templates can be designed with complementary sequences of functional modules such as aptamers, which allow DNA to specifically bind with cellular interfaces and achieve efficient cell separation. In the second part, we describe DNA supramolecular assembly on the interfaces of nanoscale particles. DNA sequences are designed with functional modules such as targeting, drug loading, and gene expression and then are assembled on interfaces of particles including upconversion nanoparticles (UCNPs), gold nanoparticles (AuNPs), and magnetic nanoparticle (MNPs). The integration of DNA with these functional particles achieves cell manipulation, targeted tumor imaging, and cellular regulation. The processes of interfacial assembly are well controlled, and the functions of the obtained bioanalytical materials can be flexibly regulated. We envision that the work on DNA supramolecular assembly on micro/nanointerfaces will be a typical paradigm for the construction of more bioanalytical materials, which we hope will facilitate the development of precision medicine.

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“…Refractive-index sensing − is a label-free, low-cost single-nanoparticle detection scheme. The key mechanism involves a perturbation to an optical cavity caused by the refractive analyte, leading to resonance shift, mode splitting/broadening, and transmitted/reflected intensity change.…”

Section: Introductionmentioning

confidence: 99%

Directivity-Enhanced Detection of a Single Nanoparticle Using a Plasmonic Slot Antenna

Lou

et al. 2022

Nano Lett.

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In situ refractive index sensors integrated with nanoaperture-based optical tweezers possess stable and sensitive responsivity to single nanoparticles. In most existing works, detection events are only identified using the total light intensity with directivity information ignored, leading to a low signal-to-noise ratio. Here, we propose to detect an optically trapped 20 nm silica particle by monitoring directivity of a plasmonic antenna. The main and secondary radiation lobes of the antenna reverse upon trapping because the particle-induced perturbation negates the relative phase between two antenna elements, leading to a significant change of the antenna front-to-back ratio. As a result, we obtain a signal-to-noise ratio of 20, with an order-of-magnitude improvement as compared to the intensity-only detection scheme.

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DNA-Modified Plasmonic Sensor for the Direct Detection of Virus Biomarkers from the Blood

Cited by 28 publications

References 42 publications

A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices

A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices

DNA Supramolecular Assembly on Micro/Nanointerfaces for Bioanalysis

Directivity-Enhanced Detection of a Single Nanoparticle Using a Plasmonic Slot Antenna

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