2013
DOI: 10.1186/1556-276x-8-87
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DNA molecule stretching through thermo-electrophoresis and thermal convection in a heated converging-diverging microchannel

Abstract: A novel DNA molecule stretching technique is developed and tested herein. Through a heated converging-diverging microchannel, thermal convection and thermophoresis induced by regional heating are shown to significantly elongate single DNA molecules; they are visualized via a confocal laser scanning microscopy. In addition, electrophoretic stretching is also implemented to examine the hybrid effect on the conformation and dynamics of single DNA molecules. The physical properties of the DNA molecules are secured… Show more

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Cited by 5 publications
(8 citation statements)
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“…We repeat these same experimental details on a standard material to have some dimension information about the effective surface area between the metal and the standard sample which gives the effective surface area between the metal and DNA. The thiol contact points on DNA serves to main jobs: (1) they carry out good metallic-DNA contacts and (2) they fix the molecule through eight fixing points which enable constant effective surface area between the metal and DNA.The expansion coefficient of DNA is 2 Å/°C [40] which makes 0.5% of error percentage. This is an acceptable tolerance of experimental measurements.…”
Section: Methodsmentioning
confidence: 99%
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“…We repeat these same experimental details on a standard material to have some dimension information about the effective surface area between the metal and the standard sample which gives the effective surface area between the metal and DNA. The thiol contact points on DNA serves to main jobs: (1) they carry out good metallic-DNA contacts and (2) they fix the molecule through eight fixing points which enable constant effective surface area between the metal and DNA.The expansion coefficient of DNA is 2 Å/°C [40] which makes 0.5% of error percentage. This is an acceptable tolerance of experimental measurements.…”
Section: Methodsmentioning
confidence: 99%
“…The thiol contact points on DNA serves to main jobs: (1) they carry out good metallic-DNA contacts and (2) they fix the molecule through eight fixing points which enable constant effective surface area between the metal and DNA. The expansion coefficient of DNA is 2 Å/°C [ 40 ] which makes 0.5% of error percentage. This is an acceptable tolerance of experimental measurements.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…In fact, engineering DNA stretching would be a key step in the development of the next generation of biological microfluidic devices [1,2]. …”
Section: Introductionmentioning
confidence: 99%
“…Если корректно дефинировать, спектрофорез не ограничен спектроэлектрофорезом, то есть другие типы фореза также можно использовать в качестве термоопосредованного спектрофореза. В частности, термоэлектрофорез и собственно термофорез [33][34][35] также могут быть предметом спектрального или спектрозонального исследования при сепарации, следовательно, спектрофореза. С другой стороны, если учитывать широкую дефиницию электрокинетических или электродисперсных процессов, возможно и в качестве «спектроэлефтрофореза», в определенном смысле слова (sensu stricto) рассматривать гальванофорез [36,37] при его спектральном или спектрозональном контроле.…”
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