DNA Nanoflowers for Multiplexed Cellular Imaging and Traceable Targeted Drug Delivery

Hu, Rong; Zhang, Xiaobing; Zhao, Zilong; Zhu, Guizhi; Chen, Tao; Fu, Ting; Tan, Weihong

doi:10.1002/anie.201400323

Cited by 298 publications

(249 citation statements)

References 33 publications

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“…The template can be customized so that the DNA product includes functional sequences such as DNA aptamers, DNAzymes, spacer domains, restriction enzyme sites and G-quadruplexes. [26][27][28] The AS1411 aptamer specifically recognizes nucleolin, which is overexpressed in various cancer cells, including breast cancer, lymphocytic leukemia, hepatocellular carcinoma, prostate carcinoma and renal cell carcinoma. 25 We expected that the numerous aptamers in the elongated ssDNA would enhance the binding of the DNFs to target cells through multivalent interaction with the nucleolin.…”

Section: Construction and Characterization Of The Dnfsmentioning

confidence: 99%

Biodegradable, multifunctional DNAzyme nanoflowers for enhanced cancer therapy

Jin

Liu

et al. 2017

NPG Asia Mater

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Safety and efficiency remain the critical hurdles hindering the practical application of gene agents, even though great effort has been made to develop various gene carriers. Herein, we present a novel biodegradable cancer therapeutic system based on DNA nanoflowers (DNFs) for targeted dual gene silencing. The therapeutic system was constructed by copying a rolling circle amplification template to produce long single-stranded DNAs with cell targeting and dual gene-silencing capability. The structure of the DNFs collapsed at acidic pH due to the decomposition of the co-assembled magnesium pyrophosphate, generating Mg 2+ ions that act as cofactors for the DNAzymes and increase their ability to recognize and cleave target mRNAs. In vitro and in vivo studies demonstrated that the multifunctional DNFs showed promise for targeted cancer cell recognition, gene silencing, induction of apoptosis and inhibition of tumor growth. Considering the enhanced therapeutic effect and biocompatibility of this therapeutic platform, it is anticipated to be of great interest for the clinical treatment of cancers.

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Section: Construction and Characterization Of The Dnfsmentioning

confidence: 99%

Biodegradable, multifunctional DNAzyme nanoflowers for enhanced cancer therapy

Jin

Liu

et al. 2017

NPG Asia Mater

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“…A cationic delivery vehicle or nanostructured DNA is required for intracellular detection. [32][33][34] Since nano-sized GO is readily internalized by mammalian cells, GO-based sensors can be directly used for intracellular detection. 35,36 Furthermore, GO protects adsorbed DNA from degradation by nucleases, 37 which is also important for detection in biological fluids.…”

Section: Introductionmentioning

confidence: 99%

Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement

et al. 2014

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Fluorescent aptamer probes physisorbed on graphene oxide (GO) have recently emerged as a useful sensing platform. Signal is generated by analyte-induced probe desorption. To address non-specific probe displacement and false positive signal, we herein report a covalently linked aptamer probe for ATP detection. A fluorophore and amino dual-modified aptamer was linked to the carboxyl group on GO with a coupling efficiency of ~50%. The linearity, specificity, stability, and regeneration of the covalent sensor was systematically studied and compared to the physisorbed probe. Both sensors have similar sensitivity, but the covalent one is more resistant to non-specific probe displacement by proteins. The covalent sensor has a dynamic range from 0.125 mM to 2 mM ATP in buffer at room temperature and is resistance to DNase I. Intracellular ATP imaging was demonstrated using the covalent sensor, which generated higher fluorescence signal than the physisorbed sensor. After stimulating the cells with 5 mM Ca 2+ for ATP production, the intracellular signal enhanced by 31.8%. This work highlights the advantages of covalent aptamer sensors using GO as both a quencher and a delivery vehicle for intracellular metabolite detection.3

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“…Using a similar approach with nanoparticles, Zheng et al (38) conjugated aptamers to gold nanospheres and hybridized aptamer-recognition elements using nanoflare techniques to achieve sensing for intracellular molecules, such as adenosine triphosphate (ATP), the most important molecule regulating cellular metabolism. Hu et al (39) reported using another aptamer-nanomaterial complex (known as DNA nanoflowers) to perform cell imaging and drug-delivery tracing in cells. These…”

Section: Aptamer-based Biosensorsmentioning

confidence: 99%

Biosensors for Cell Analysis

Zhou

Son

Liu

et al. 2015

Annu. Rev. Biomed. Eng.

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Biosensors first appeared several decades ago to address the need for monitoring physiological parameters such as oxygen or glucose in biological fluids such as blood. More recently, a new wave of biosensors has emerged in order to provide more nuanced and granular information about the composition and function of living cells. Such biosensors exist at the confluence of technology and medicine and often strive to connect cell phenotype or function to physiological or pathophysiological processes. Our review aims to describe some of the key technological aspects of biosensors being developed for cell analysis. The technological aspects covered in our review include biorecognition elements used for biosensor construction, methods for integrating cells with biosensors, approaches to single-cell analysis, and the use of nanostructured biosensors for cell analysis. Our hope is that the spectrum of possibilities for cell analysis described in this review may pique the interest of biomedical scientists and engineers and may spur new collaborations in the area of using biosensors for cell analysis.

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DNA Nanoflowers for Multiplexed Cellular Imaging and Traceable Targeted Drug Delivery

Cited by 298 publications

References 33 publications

Biodegradable, multifunctional DNAzyme nanoflowers for enhanced cancer therapy

Biodegradable, multifunctional DNAzyme nanoflowers for enhanced cancer therapy

Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement

Biosensors for Cell Analysis

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