DNA Nanotechnology‐Based Biosensors and Therapeutics

Shen, Liwei; Wang, Pengfei; Ke, Yonggang

doi:10.1002/adhm.202002205

Cited by 78 publications

(66 citation statements)

References 290 publications

(223 reference statements)

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“…Most recently, nucleic acid-based fluorescent nanoprobes have emerged as a powerful tool for detection of ATP. − Capitalizing on specific recognition of molecular targets and catalytic activities, − functional nucleic acids, such as aptamers and DNAzymes, have been engineered into various designs. Importantly, DNA nanosensors have demonstrated unique advantages, such as high cellular uptake and resistance to degradation. , Despite the progress made, a major challenge for such strategies is that these probes lack cancer-cell selectivity due to the wide distribution of ATP in both normal and cancer cells, hampering their use for reliable imaging of cancer cells.…”

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confidence: 99%

A Cooperatively Activatable DNA Nanoprobe for Cancer Cell-Selective Imaging of ATP

Zhou

Zou

et al. 2021

Anal. Chem.

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DNA-based nanoprobes have attracted extensive interest in the field of bioanalysis. Notably, engineered DNA nanoprobes that can respond to multiple pathological parameters are desirable to detect targets precisely. Here we design a split aptamer/DNAzyme (aptazyme)-based DNA probe for fluorescence detection of ATP and further develop a cooperatively activatable DNA nanoprobe for tumor-specific imaging of ATP in vivo. The DNA nanoprobes comprising split aptazyme-coated MnO 2 nanovectors have high stability and are synergistically activated by multiple biomarkers, GSH and ATP. Upon stimuli by overexpressed GSH in tumor cells, this DNA nanoprobe can release the aptazyme and self-supply cofactor Mn 2+ of the DNAzyme. Sequentially, intracellular ATP induces the proper folding of the split ATP aptamer and Mn 2+ -dependent DNAzyme, which activates the specific cleavage of substrate and generates the optical readout signal. This nanoprobe exhibits remarkable resistance to enzymatic degradation, satisfactory biosafety, identifies ATP specifically within cancer cells, and selectively lights up solid tumors. Our research provides a reliable method for ATP imaging in cancer cells and opens a new avenue for biochemical research and highly accurate disease diagnosis.

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confidence: 99%

A Cooperatively Activatable DNA Nanoprobe for Cancer Cell-Selective Imaging of ATP

Zhou

Zou

et al. 2021

Anal. Chem.

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“…DNA nanostructures have also been demonstrated as excellent biocompatible nanocarriers showing programmable geometries and functions for effective cancer therapy [ 219 – 221 ]. In the field of phototherapy, photothermal and photodynamic components can be anchored onto DNA templates for precise and effective PTT and PDT, respectively [ 222 ]. In one study, the gold nanorod (AuNR) [ 223 ], a frequently used photothermal agent, was attached to DNA origami nanostructures for promising photothermal treatment of breast cancer in mice [ 224 ] and enhanced photoacoustic imaging [ 225 ] (Fig.…”

Section: Photonic Systems Directed By Structural Dna Templatesmentioning

confidence: 99%

Interfacing DNA nanotechnology and biomimetic photonic complexes: advances and prospects in energy and biomedicine

2022

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Self-assembled photonic systems with well-organized spatial arrangement and engineered optical properties can be used as efficient energy materials and as effective biomedical agents. The lessons learned from natural light-harvesting antennas have inspired the design and synthesis of a series of biomimetic photonic complexes, including those containing strongly coupled dye aggregates with dense molecular packing and unique spectroscopic features. These photoactive components provide excellent features that could be coupled to multiple applications including light-harvesting, energy transfer, biosensing, bioimaging, and cancer therapy. Meanwhile, nanoscale DNA assemblies have been employed as programmable and addressable templates to guide the formation of DNA-directed multi-pigment complexes, which can be used to enhance the complexity and precision of artificial photonic systems and show the potential for energy and biomedical applications. This review focuses on the interface of DNA nanotechnology and biomimetic photonic systems. We summarized the recent progress in the design, synthesis, and applications of bioinspired photonic systems, highlighted the advantages of the utilization of DNA nanostructures, and discussed the challenges and opportunities they provide. Graphical Abstract

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“…Among these, DNA nanotechnology has received the most attention from researchers interested in the material rather than genetic characteristics of DNA [ 9 , 10 ]. DNA nanotechnology has brought forth a new paradigm for DNA research, one that employs the genetic functionality of DNA for engineering applications in biosensors [ 11 , 12 ], nanoarchitecture [ 13 , 14 ], drug delivery [ 15 , 16 ], and computations [ 17 , 18 ]. This integrated approach has resulted in the development of a tremendous line of products, which would have been impossible with conventional nucleic acid research or nanobiotechnology alone.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in DNA Nanotechnology for Plasmonic Biosensor Construction

et al. 2022

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Since 2010, DNA nanotechnology has advanced rapidly, helping overcome limitations in the use of DNA solely as genetic material. DNA nanotechnology has thus helped develop a new method for the construction of biosensors. Among bioprobe materials for biosensors, nucleic acids have shown several advantages. First, it has a complementary sequence for hybridizing the target gene. Second, DNA has various functionalities, such as DNAzymes, DNA junctions or aptamers, because of its unique folded structures with specific sequences. Third, functional groups, such as thiols, amines, or other fluorophores, can easily be introduced into DNA at the 5′ or 3′ end. Finally, DNA can easily be tailored by making junctions or origami structures; these unique structures extend the DNA arm and create a multi-functional bioprobe. Meanwhile, nanomaterials have also been used to advance plasmonic biosensor technologies. Nanomaterials provide various biosensing platforms with high sensitivity and selectivity. Several plasmonic biosensor types have been fabricated, such as surface plasmons, and Raman-based or metal-enhanced biosensors. Introducing DNA nanotechnology to plasmonic biosensors has brought in sight new horizons in the fields of biosensors and nanobiotechnology. This review discusses the recent progress of DNA nanotechnology-based plasmonic biosensors.

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DNA Nanotechnology‐Based Biosensors and Therapeutics

Cited by 78 publications

References 290 publications

A Cooperatively Activatable DNA Nanoprobe for Cancer Cell-Selective Imaging of ATP

A Cooperatively Activatable DNA Nanoprobe for Cancer Cell-Selective Imaging of ATP

Interfacing DNA nanotechnology and biomimetic photonic complexes: advances and prospects in energy and biomedicine

Recent Advances in DNA Nanotechnology for Plasmonic Biosensor Construction

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