DNA ploidy and cell-cycle analysis in intracranial meningiomas and hemangiopericytomas: A study with high-resolution DNA flow cytometry

Zellner, Anton; Meixensberger, Jürgen; Roggendorf, Wolfgang; Janka, Michael; Hoehn, Holger; Roosen, K.

doi:10.1002/(sici)1097-0215(19980417)79:2<116::aid-ijc3>3.0.co;2-w

Cited by 17 publications

(8 citation statements)

References 28 publications

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“…Besides, there was a significant difference in G0/G1 phase, S phase and mitoses fraction between benign and atypical/anaplastic meningiomas. Zellner et al have found that atypical meningiomas had a significantly higher S‐phase fraction, compared to atypical/anaplastic that in benign meningiomas . Compared with benign meningiomas, atypical/anaplastic meningioma had significant lower G0/G1‐phase along with higher S‐phase fractions.…”

Section: Discussionmentioning

confidence: 99%

“…The DNA polid and cell cycle phase determined by flow cytometry are useful indicators for assessing biological behaviors in meningiomas . Besides, there was a significant difference in G0/G1 phase, S phase and mitoses fraction between benign and atypical/anaplastic meningiomas.…”

Section: Discussionmentioning

confidence: 99%

“…Although several DNA flow cytometric studies have been performed for meningiomas, conflicting results have been found in the distribution of the cell cycle phases and various . The reasons for variance might be different cell preparation methods, instrumentation, and flow cytometry, including histogram evaluation.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have demonstrated that G0/G1‐phase and S‐phase fraction, as determined by flow cytometry, are useful indicators of different biological behaviors in meningiomas. Meanwhile, a correlation between S‐phase fraction and survival as well as S‐phase fraction and recurrence are also observed in meningiomas . Therefore, the cell cycle kinetic data appear suited to distinguish between benign and atypical/anaplastic meningiomas and also to predict the risk of tumoral recurrence.…”

mentioning

confidence: 99%

“…In human meningiomas, several studies have shown that increased Ki‐67 level was positively associated with higher tumor grade and increased risk of recurrence . Furthermore, flow cytometric analysis of cell tumoral cell cycle has also been applied to evaluate the aggressiveness of meningiomas since flow cytometry has been thought to provide more sensitive and objective information on the proliferative rate of tumor cells than the data of traditional histology . Previous studies have demonstrated that G0/G1‐phase and S‐phase fraction, as determined by flow cytometry, are useful indicators of different biological behaviors in meningiomas.…”

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confidence: 99%

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The application of flow cytometry for evaluating biological aggressiveness of intracranial meningiomas

Lin

Tai

Huang

et al. 2014

Cytometry Part B Clinical

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Background: Meningiomas have classically been considered to include benign and atypical/anaplastic tumors. Despite the availability of clinical and pathologic parameters for prognostic prediction prognosis, the behavior of each meningioma may be difficult to predict. Here, we used DNA flow-cytometric studies to predict biological tumor behaviors of intracranial meningiomas.Methods: The specimens were obtained from fresh tumoral tissues of 43 microsurgically resected meningiomas as approved by the institutional review board. The presence of G2/M-phase and S1G2/Mphase fractions were analyzed and correlated with the proliferation index of Ki-67 and the World Health Organization grading. The check point of G2/M-phase fraction, cyclin B, and pCdk1 (Y15), were analyzed by Western blotting.Results: Our results showed that there were significant differences in Ki-67, G2/M-phase, S1G2/Mphase fractions, and cyclin B between benign and atypical/anaplastic meningiomas. The optimal cutoff point of G2/M-phase and S1G2/M-phase fractions were 5.12 and 7.52%, respectively, and this can be used to discriminate those cases with benign or atypical/anaplastic meningiomas. Besides, both the G2/ M-phase and S1G2/M-phase fractions were correlated well with Ki-67 and the histopathological features such as focal necrosis, infiltration of dura mater and mitotic activity. In addition, the occurrence of tumor recurrence and patient age were correlated to the G2/M-phase and S1G2/M-phase fractions, respectively. The G2/M-phase and S1G2/M-phase fractions, however, did not correlate well with histologic invasion to adjacent bone, sinus, or brain tissues.Conclusions: The use of flow cytometry facilitates additional information for G2/M-phase and S1G2/Mphase fractions represent tumoral grading and risk of recurrence in patients with meningiomas. V C 2014

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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