The application of flow cytometry for evaluating biological aggressiveness of intracranial meningiomas

Lin, Yu‐Wen; Tai, Shih‐Huang; Huang, Yu‐Hsuan; Chang, Chia Ling; Juan, Wei-Sheng; Chao, Liang-Chun; Wen, Miin-Jye; Hung, Yao-Min; Lee, E-Jian

doi:10.1002/cyto.b.21202

Cited by 12 publications

(5 citation statements)

References 39 publications

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“…To overcome these current limitations, the present study was initiated to assess the value of iFC in the treatment of meningiomas. There have been some pilot studies regarding the effectiveness of flow cytometry in investigating the biological aggressiveness of meningiomas . With the aid of a newly developed kit solution, iFC has become readily available .…”

Section: Discussionmentioning

confidence: 99%

Intraoperative quantification of meningioma cell proliferation potential using rapid flow cytometry reveals intratumoral heterogeneity

et al. 2019

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Background Standard sampling methods to evaluate the proliferative ability of meningioma have not been established. Methods This prospective study was conducted to evaluate the effectiveness of intraoperative rapid flow cytometry (iFC) using raw samples for the quantitative assessment of proliferative ability in meningioma cells and to investigate intratumoral heterogeneity. Proliferation index (PI) was defined as the ratio of aneuploid cells with an abnormal number of chromosomes to the total cells. Results From 50 patients, 118 specimens were analyzed. There was a statistically significant correlation between the postoperative MIB‐1 labeling index (LI) and PI (R = 0.59, P < 0.0001). A higher PI was correlated with a higher annual growth rate (AGR, cm3/y) (R = 0.50, P = 0.0002, 26 patients). AGR showed a correlation with the intratumoral distribution of PI. PI was the highest at the center or the peripheral section of the tumor in tumors with high AGR, whereas it was highest at the dural attachment in tumors with low AGR (P = 0.039, n = 20). Pial feeders were more frequently observed when PI was high in the center or in the peripheral section (P = 0.006, n = 37). Conclusions Rapid iFC may thus become a substitute for MIB‐1 LI. Intratumoral heterogeneity of cellular proliferative potential exists in meningiomas and is related to tumor biological characteristics such as AGR and development of pial feeders. This observation underscores the importance of standardization in the sampling method to accurately estimate the risk of meningioma recurrence.

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Section: Discussionmentioning

confidence: 99%

Intraoperative quantification of meningioma cell proliferation potential using rapid flow cytometry reveals intratumoral heterogeneity

et al. 2019

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“…We read with great interest the recent article by Lin et alon the value of flow cytometry in predicting biological tumor behaviors of intracranial meningiomas (1). Meningiomas are among the most frequent benign intracranial tumors, accounting for 30% of all primary intracranial tumors.…”

Section: Dear Editormentioning

confidence: 99%

Is there a role for intraoperative flow cytometry in brain tumor surgery?

Vartholomatos

Alexiou

Gerogianni

et al. 2015

Cytometry Part B Clinical

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“…Ploidy status was not significantly associated with recurrence-free survival [21]. Lin et al, in a study of 43 patients with meningioma, found that the best cutoff value for the discrimination of grade 1 from grade 2 meningiomas was the G 2 /M-phase and S + G 2 /M-phase fractions of 5.12 and 7.52%, respectively [22]. Sampling was always performed from the tumor's core.…”

Section: Discussionmentioning

confidence: 99%

Intraoperative Flow Cytometry for the Evaluation of Meningioma Grade

et al. 2023

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Meningiomas are the most frequent central nervous system tumors in adults. The majority of these tumors are benign. Nevertheless, the intraoperative identification of meningioma grade is important for modifying surgical strategy in order to reduce postoperative complications. Here, we set out to investigate the role of intraoperative flow cytometry for the differentiation of low-grade (grade 1) from high-grade (grade 2–3) meningiomas. The study included 59 patients. Intraoperative flow cytometry analysis was performed using the ‘Ioannina Protocol’ which evaluates the G0/G1 phase, S-phase, mitosis and tumor index (S + mitosis phase fraction) of a tumor sample. The results are available within 5 min of sample receipt. There were 41 grade 1, 15 grade 2 and 3 grade 3 meningiomas. High-grade meningiomas had significantly higher S-phase fraction, mitosis fraction and tumor index compared to low-grade meningiomas. High-grade meningiomas had significantly lower G0/G1 phase fraction compared to low-grade meningiomas. Thirty-eight tumors were diploids and twenty-one were aneuploids. No significant difference was found between ploidy status and meningioma grade. ROC analysis indicated 11.4% of tumor index as the optimal cutoff value thresholding the discrimination between low- and high-grade meningiomas with 90.2% sensitivity and 72.2% specificity. In conclusion, intraoperative flow cytometry permits the detection of high-grade meningiomas within 5 min. Thus, surgeons may modify tumor removal strategy.

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The application of flow cytometry for evaluating biological aggressiveness of intracranial meningiomas

Cited by 12 publications

References 39 publications

Intraoperative quantification of meningioma cell proliferation potential using rapid flow cytometry reveals intratumoral heterogeneity

Intraoperative quantification of meningioma cell proliferation potential using rapid flow cytometry reveals intratumoral heterogeneity

Is there a role for intraoperative flow cytometry in brain tumor surgery?

Intraoperative Flow Cytometry for the Evaluation of Meningioma Grade

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