DNA Ploidy and S-phase fraction, but not p53 or NM23-H1 expression, predict outcome in colorectal cancer patients. Result of a 5-year prospective study

Bazan, V.; Migliavacca, Manuela; Zanna, Ines; Tubiolo, Carla; Corsale, S; Calò, Valentina; Amato, Antonella; Cammareri, Patrizia; Latteri, Federica; Grassi, Nello; Fulfaro, Fabio; Porcasi, Rossana; Morello, Vincenza; Nuara, R; Dardanoni, Gabriella; Salerno, Sergio; Valerio, Maria Rosaria; Dusonchet, Luisa; Gerbino, Aldo; Gebbia, Nicola; Rm, Tomasino; Russo, Antonio

doi:10.1007/s00432-002-0394-6

Cited by 40 publications

(7 citation statements)

References 54 publications

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“…Cancer patients exhibited higher serum NDKA levels as expected, in line with the findings reported by our group and others for the protein in tumour tissue compared to normal mucosa using Western blot or immunohistochemistry techniques26282931, as well as for mRNA by Northern blot and RT-PCR242527. Regarding the characteristics of the tumour, we did not find any association between serum NDKA and tumour stage, TNM classification, differentiation grade or location, consistent with that reported by others analysing NDKA expression in tumour tissue2328343536.…”

Section: Discussionsupporting

confidence: 92%

Evaluation of serum nucleoside diphosphate kinase A for the detection of colorectal cancer

Otero-Estévez

Chiara

Barcia-Castro

et al. 2016

Sci Rep

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We previously described the over-expression of nucleoside diphosphate kinase A (NDKA) in tumours and serum from colorectal cancer (CRC) patients, suggesting its use as biomarker. In this study we evaluated the diagnostic accuracy of serum NDKA to detect advanced neoplasia (CRC or advanced adenomas). Furthermore, the performance of NDKA was compared with the faecal immunochemical test (FIT). The study population included a case-control cohort and a screening cohort (511 asymptomatic first-degree relatives of CRC patients that underwent a colonoscopy and a FIT). Serum NDKA was elevated in CRC patients in the case-control cohort (p = 0.002). In the screening cohort, NDKA levels were higher for advanced adenomas (p = 0.010) and advanced neoplasia (p = 0.006) compared to no neoplasia. Moreover, elevated NDKA was associated with severe characteristics of adenomas (≥3 lesions, size ≥ 1 cm or villous component). Setting specificity to 85%, NDKA showed a sensitivity of 30.19% and 29.82% for advanced adenomas and advanced neoplasia, respectively. NDKA combined with FIT (100 ng/mL cut-off) detected advanced adenomas and advanced neoplasia with 45.28% and 49.12% sensitivity, with specificity close to 90%. The combination of serum NDKA and FIT can improve the detection of advanced neoplasia, mainly for lesions located on the proximal colon, in asymptomatic individuals with CRC family-risk.

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Section: Discussionsupporting

confidence: 92%

Evaluation of serum nucleoside diphosphate kinase A for the detection of colorectal cancer

Otero-Estévez

Chiara

Barcia-Castro

et al. 2016

Sci Rep

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“…To estimate the rate of cell proliferation, the SPF was frequently used in breast cancer, thyroid cancer and oral leukoplakia [21-23]. In colon cancer, high SPF was significantly associated with a poor prognosis [24,25]. We also found that knockdown of CNDP2 reduced cyclin B1 expression, which increased the levels of G2.…”

Section: Discussionmentioning

confidence: 87%

Up-regulation of CNDP2 facilitates the proliferation of colon cancer

Xue

Zhang

et al. 2014

BMC Gastroenterol

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BackgroundCytosolic nonspecific dipetidase (CN2) belongs to the family of M20 metallopeptidases. It was stated in previous articles that higher expression levels of CN2 were observed in renal cell carcinoma and breast cancer. Our study explored the correlation between CN2 and colon carcinogenesis.MethodsWe analysed the relationship between 183 patients clinicopathological characteristics and its CN2 expression. To detect the levels of CN2 in colon cancer cell lines and colon cancer tissues by western blot. To verify cell proliferation in colon cancer cells with knockdown of CNDP2 and explore the causes of these phenomena.ResultsThe expression levels of CN2 in clinical colon tumors and colon cancer cell lines were significantly higher than that in normal colon mucosa and colon cell lines. The difference in CN2 levels was associated with tumor location (right- and left-sided colon cancer), but there was no significant association with age, gender, tumor size, tumor grade, tumor stage or serum carcinoembryonic antigen (CEA). Knockdown of CNDP2 inhibited cell proliferation, blocked cell cycle progression and retarded carcinogenesis in an animal model. The signaling pathway through which knockdown of CNDP2 inhibited cell proliferation and tumorigenesis involved in EGFR, cyclin B1 and cyclin E.ConclusionsKnockdown of CNDP2 can inhibit the proliferation of colon cancer in vitro and retarded carcinogenesis in vivo.

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“…A DNA ploidy pattern has been proved to be an important prognostic factor. Diploid tumors conferred a better survival compared with aneuploid tumors (Bazan et al, 2002;Lanza et al, 1998). Tumors of the proximal colon are more frequently diploid than those localized distally to the splenic flexure.…”

Section: Tnm Staging and Prognosismentioning

confidence: 97%

Colorectal cancer, one entity or three

Lai

2009

J. Zhejiang Univ. Sci. B

216

163

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Abstract:Understanding of the mechanism of colorectal carcinogenesis has been gaining momentum for some years on account of its high incidence and impact on the lives of individuals affected. Different genetic abnormalities have been found in colorectal cancers from different sites. For example, proximal colon cancer is usually related to the nucleotide instability pathway, as microsatellite instability (MSI). However, distal colon cancer is usually associated with specific chromosomal instability (CIN). The development of cancer at the rectum, though similar to that at the colon, displays its own unique features. These differences might be partially attributed to different embryological development and physiological circumstances. Environmental factors such as diet and alcohol intake also differ in their role in the development of tumors in the three segments, proximal colon, distal colon, and rectum. "Proximal shift" of colon cancer has been known for some time, and survival rates of colorectal cancer are higher when rectal cancers are excluded, both of which emphasize the three different segments of colorectal cancer and their different properties. Meanwhile, colonic and rectal cancers are distinctive therapeutic entities. The concept of three entities of colorectal cancer may be important in designing clinical trails or therapeutic strategies. However, the dispute about the inconsistency of data concerning the site-specific mechanism of colorectal carcinoma does exist, and more evidence about molecular events of carcinogenesis and targeted therapy needs to be collected to definitely confirm the conception.

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DNA Ploidy and S-phase fraction, but not p53 or NM23-H1 expression, predict outcome in colorectal cancer patients. Result of a 5-year prospective study

Abstract: Our results indicate that DNA aneuploidy and high SPF are associated in CRC with a poor clinical 5-year outcome, while in contrast the prognostic role of TP53 and NM23-H1 expression is still to be clarified.

Cited by 40 publications

References 54 publications

Evaluation of serum nucleoside diphosphate kinase A for the detection of colorectal cancer

Evaluation of serum nucleoside diphosphate kinase A for the detection of colorectal cancer

Up-regulation of CNDP2 facilitates the proliferation of colon cancer

Colorectal cancer, one entity or three

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