Up-regulation of CNDP2 facilitates the proliferation of colon cancer

Xue, Conglong; Zhang, Zhenwei; Yu, Honglan; Yu, Miao; Yuan, Kaitao; Yang, Ting; Miao, Mingyong; Hanping, Shi

doi:10.1186/1471-230x-14-96

Cited by 19 publications

(14 citation statements)

References 29 publications

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“…Many studies reported that the CNDP2 gene is misexpressed in different types of human cancers [ 2 , 6 – 11 ]. CNDP2 was also found to be involved in the regulation of the cell cycle in human cancer cell lines.…”

Section: Discussionmentioning

confidence: 99%

“…CNDP2 was also found to be involved in the regulation of the cell cycle in human cancer cell lines. Specifically, CNDP2 overexpression in pancreatic cancer lines induced the accumulation of cells in the G0/G1 phase [ 6 ], whereas knockdown of CNDP2 in colon cancer cells blocked the cell cycle progression in the G2/M phase [ 11 ]. However, the molecular mechanisms underlying these effects have not been uncovered.…”

Section: Discussionmentioning

confidence: 99%

“…However, not all tumors are characterized by low CNDP2 levels. An upregulated expression of CNDP2 has been indeed observed in breast carcinoma, and in kidney and colon cancers [ 8 – 11 ]. Several studies have been devoted to understanding the contribution of CNDP2 to carcinogenesis [ 2 , 6 – 11 ], but an animal model with mutated CNDP2 is not currently available.…”

Section: Introductionmentioning

confidence: 99%

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A toolset to study functions of Cytosolic non-specific dipeptidase 2 (CNDP2) using Drosophila as a model organism

et al. 2019

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BackgroundExpression of the CNDP2 gene is frequently up- or down-regulated in different types of human cancers. However, how the product of this gene is involved in cell growth and proliferation is poorly understood. Moreover, our knowledge of the functions of the CNDP2 orthologs in well-established model organisms is scarce. In particular, the function of the D. melanogaster ortholog of CNDP2, encoded by the CG17337 gene (hereafter referred to as dCNDP2), is still unknown.ResultsThis study was aimed at developing a set of genetic and molecular tools to study the roles of dCNDP2. We generated a dCNDP2 null mutation (hereafter ∆dCNDP2) using CRISPR/Cas9-mediated homologous recombination (HR) and found that the ∆dCNDP2 mutants are homozygous viable, morphologically normal and fertile. We also generated transgenic fly lines expressing eGFP-tagged and non-tagged dCNDP2 protein, all under the control of the UAS promoter, as well as polyclonal antibodies specific to dCNDP2. Using these tools, we demonstrate that only one of the two predicted dCNDP2 isoforms is expressed throughout the different tissues tested. dCNDP2 was detected in both the cytoplasm and the nucleus, and was found to be associated with multiple sites in the salivary gland polytene chromosomes.ConclusionsThe dCNDP2 gene is not essential for fly viability under standard laboratory conditions. The subcellular localization pattern of dCNDP2 suggests that this protein might have roles in both the cytoplasm and the nucleus. The genetic and molecular tools developed in this study will allow further functional characterization of the conserved CNDP2 protein using D. melanogaster as a model system.Electronic supplementary materialThe online version of this article (10.1186/s12863-019-0726-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A toolset to study functions of Cytosolic non-specific dipeptidase 2 (CNDP2) using Drosophila as a model organism

et al. 2019

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“…Our transcriptional analysis reveals that cndp2 , which promotes cell proliferation, is more highly expressed in EB and apical-LB blastema populations. EGFR signaling is required for CNDP2 mediated proliferative activity [ 23 ] and is activated during regeneration [ 25 ]. Additionally, EGFR activity is induced by matrix metalloprotease activity in the regenerate [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Positional plasticity in regenerating Amybstoma mexicanum limbs is associated with cell proliferation and pathways of cellular differentiation

et al. 2015

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BackgroundThe endogenous ability to dedifferentiate, re-pattern, and re-differentiate adult cells to repair or replace damaged or missing structures is exclusive to only a few tetrapod species. The Mexican axolotl is one example of these species, having the capacity to regenerate multiple adult structures including their limbs by generating a group of progenitor cells, known as the blastema, which acquire pattern and differentiate into the missing tissues. The formation of a limb regenerate is dependent on cells in the connective tissues that retain memory of their original position in the limb, and use this information to generate the pattern of the missing structure. Observations from recent and historic studies suggest that blastema cells vary in their potential to pattern distal structures during the regeneration process; some cells are plastic and can be reprogrammed to obtain new positional information while others are stable. Our previous studies showed that positional information has temporal and spatial components of variation; early bud (EB) and apical late bud (LB) blastema cells are plastic while basal-LB cells are stable. To identify the potential cellular and molecular basis of this variation, we compared these three cell populations using histological and transcriptional approaches.ResultsHistologically, the basal-LB sample showed greater tissue organization than the EB and apical-LB samples. We also observed that cell proliferation was more abundant in EB and apical-LB tissue when compared to basal-LB and mature stump tissue. Lastly, we found that genes associated with cellular differentiation were expressed more highly in the basal-LB samples.ConclusionsOur results characterize histological and transcriptional differences between EB and apical-LB tissue compared to basal-LB tissue. Combined with our results from a previous study, we hypothesize that the stability of positional information is associated with tissue organization, cell proliferation, and pathways of cellular differentiation.Electronic supplementary materialThe online version of this article (doi:10.1186/s12861-015-0095-4) contains supplementary material, which is available to authorized users.

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“…Proteins identified in regions of gain included the established EGFR oncogene 21 , and candidates such as FOXK1, a forkhead transcription factor, and CNDP2, an activator of MAPK pathways. Increased expression of FOXK1 has been shown to promote CRC invasion and metastasis 22 , and up-regulation of CNDP2 to facilitate colon cancer proliferation 23 . In regions of loss, we identified several putative tumor suppressors, including MTHFD1, a 1-tetrahydrofolate synthase.…”

Section: Resultsmentioning

confidence: 99%

Colorectal Cancer Cell Line Proteomes Are Representative of Primary Tumors and Predict Drug Sensitivity

et al. 2017

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Background and Aims Proteomics holds promise for individualizing cancer treatment. We analyzed to what extent the proteomic landscape of human colorectal cancer (CRC) is maintained in established CRC cell lines and the utility of proteomics for predicting therapeutic responses. Methods Proteomic and transcriptomic analyses were performed on 44 CRC cell lines, compared against primary CRCs (n=95) and normal tissues (n=60), and integrated with genomic and drug sensitivity data. Results Cell lines mirrored the proteomic aberrations of primary tumors, in particular for intrinsic programs. Tumor relationships of protein expression with DNA copy number aberrations and signatures of post-transcriptional regulation were recapitulated in cell lines. The five proteomic subtypes previously identified in tumors were represented among cell lines. Nonetheless, systematic differences between cell line and tumor proteomes were apparent, attributable to stroma, extrinsic signaling and growth conditions. Contribution of tumor stroma obscured signatures of DNA mismatch repair identified in cell lines with a hypermutation phenotype. Global proteomic data showed improved utility for predicting both known drug-target relationships and overall drug sensitivity as compared to genomic or transcriptomic measurements. Inhibition of targetable proteins associated with drug responses further identified corresponding synergistic or antagonistic drug combinations. Our data provide evidence for CRC proteomic subtype-specific drug responses. Conclusions Proteomes of established CRC cell line are representative of primary tumors. Proteomic data tend to exhibit improved prediction of drug sensitivity as compared to genomic and transcriptomic profiles. Our integrative proteogenomic analysis highlights the potential of proteome profiling to inform personalized cancer medicine.

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Up-regulation of CNDP2 facilitates the proliferation of colon cancer

Cited by 19 publications

References 29 publications

A toolset to study functions of Cytosolic non-specific dipeptidase 2 (CNDP2) using Drosophila as a model organism

A toolset to study functions of Cytosolic non-specific dipeptidase 2 (CNDP2) using Drosophila as a model organism

Positional plasticity in regenerating Amybstoma mexicanum limbs is associated with cell proliferation and pathways of cellular differentiation

Colorectal Cancer Cell Line Proteomes Are Representative of Primary Tumors and Predict Drug Sensitivity

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