DNA Polymerase Induced by Simian Virus 40

Kit, Saul; Piekarski, L; Dubbs, D. R.

doi:10.1099/0022-1317-1-2-163

Cited by 33 publications

(21 citation statements)

References 16 publications

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“…Neither intact cells nor extracts of the transformed cells produced plaques when plated W40-INFECTE on CV-1 cells. However, small amounts of SV40 OUSE KIDNEY could be recovered routinely when the trans-NA KS-A DNA formed cells were grown in mixed cultures with ARKER CV-1 cells (25,26). One strain (mKS-A) of the SV40-transformed mouse kidney cells (Table 1; experiment I) has been tested for the possible presence of twistedcircular SV40 DNA.…”

Section: Sedimentation Velocity Analysis (Band Centrifugation)mentioning

confidence: 99%

“…In SV40-infected cultures, the incorporation of ,ular-SV40 3H-dT into DNA was markedly stimulated. Since ures aborthe activities dT kinase, DNA polymerase, dTMP lustrates a synthetase, and FH2 reductase are also enhanced ent with after SV40 infection (11,23,25,26), it would not ise kidney be unreasonable to suppose that labeled DNA edimented precursor pools increase in infected cultures and irker. The that an increase in 3H-dTTP pools partly explains rom uninthe increased incorporation of 3H-dT into DNA.…”

Section: Culturesmentioning

confidence: 99%

See 1 more Smart Citation

Induction of Cellular Deoxyribonuleic Acid Synthesis by Simian Virus 40

Kit

Torres

Dubbs

et al. 1967

J Virol

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The incorporation of 3 H-thymidine ( 3 H-dT) into deoxyribonucleic acid (DNA) has been studied in uninfected confluent monolayer cultures of monkey kidney and mouse kidney cells, simian virus 40 (SV40)-infected cells, and in SV40-transformed mouse kidney cells. Radioautographic measurements revealed that during the period from 28 to 51 hr after productive SV40 infection of monkey kidney cultures about 80% of the cells synthesized DNA, compared to about 16% in uninfected cultures. At 28 to 43 hr after abortive SV40 infection of mouse kidney cultures, 24 to 37% of the cells synthesized DNA, compared to about 6 to 8% in uninfected cultures. The infected monkey kidney and mouse kidney cultures, respectively, incorporated about 5 to 10 times and 3 to 5 times as much 3 H-dT into DNA as did uninfected cultures. Moreover, the net DNA synthesized by SV40-infected monkey kidney cultures, estimated by colorimetric methods, substantially exceeded that of uninfected cultures. Nitrocellulose chromatography and band centrifugation experiments were performed to elucidate the kinds of DNA synthesized in the cultures. In uninfected monkey kidney cultures and at 2 to 12 hr after SV40 infection, almost all of the 3 H-dT labeled DNA sedimented more rapidly than SV40 DNA, and the radioactive DNA was denatured by heating for 12 min at 100 C (cellular DNA). Almost all of the labeled DNA obtained from abortively infected mouse kidney cultures and from SV40-transformed cells also had the properties of cellular DNA. However, approximately one-third to one-half of the labeled DNA obtained from monkey kidney cultures 28 to 51 hr after infection sedimented more slowly than cellular DNA and was not denatured by the heating (SV40 DNA). It is concluded that cellular DNA synthesis was induced during either the productive or abortive SV40 infections.

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Section: Sedimentation Velocity Analysis (Band Centrifugation)mentioning

confidence: 99%

Section: Culturesmentioning

confidence: 99%

Induction of Cellular Deoxyribonuleic Acid Synthesis by Simian Virus 40

Kit

Torres

Dubbs

et al. 1967

J Virol

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“…DDDP has been shown to be increased following infection of cells by a variety of DNA viruses (pox viruses, herpes viruses, polyoma, SV 15, SV 40) (23). Productive infection is commonly associated with the appearance of a virus-specific polymerase, whereas transforming infection appears to effect a derepression of a normal cellular DDDP activ- ity.…”

Section: Discussionmentioning

confidence: 99%

“…These abnormalities have been observed in cells passed numerous times in tissue culture, suggesting that the abnormalities are due to a genetic alteration in RA synovial cells. Chronic viral infection is a likely pathogenic mechanism for such an alteration, and numerous persistent biologic and biochemical abnormalities have been associated with chronic virus infection and/or transformation of cells (23)(24)(25).…”

Section: Discussionmentioning

confidence: 99%

DNA polymerase activity of cultured rheumatoid synovial cells

Spruance

Richards

Smith

et al. 1975

Arthritis & Rheumatism

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Cultured synovial cells from 8 patients with rheumatoid arthritis (RA) and 7 subjects without joint disease were assayed and compared for RNA‐directed and DNA‐directed DNA polymerase activity. No activity was found in either RA or control specimens using a synthetic RNA template that specifically detects oncornavirus RNA‐directed DNA polymerase. The DNA‐directed DNA polymerase activity of RA specimens was increased (P <0.10) in the high‐speed pellet fraction of cell lysates. The possible relationship of these finding to virus infection in RA is discussed.

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“…There is also a substantial rise in the activities of enzymes associated with DNA synthesis, such us DNA polymerase (i, 4--8), thymidine kinase (1--4), dCMP deaminase (1), dTMP kinase (4) and I?~NA polymerase (9). Although several enzymes, isolated from viral-infected ceils, exhibited physio-ehemical properties different from those of noninfectecl cells (3,6,9), the limited coding capacity of the viral genome (10) as well as immunological evidence (8) tend to rule out the possibility of viral induced enzymes as such.…”

Section: Introductionmentioning

confidence: 98%

Fluctuation in activity of the molecular forms of cellular DNA polymerase during infection by SV40

Lazarus

Kitron

1976

Archives of Virology

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Infection of BSC-1 cells by SV40 brings about an increase of 7--11-fold in DNA polymerase activity, found in the nuclei and cytoplasm, respectively. The overall ratio between activites of DNA polymerase beta (3.1S) and DNA polymerase alpha (5.5S) remains fairly constant throughout infection. However,there is a large increase in DNA polymerase alpha2 (7.1S) in the cytoplasm, and its appearance in the nuclei late in infection. The addition of 1 M NaCl to infected cytoplasm,causes an aggregation of DNA polymerase alpha into a higher sedimenting form (9.8S), termed DNA polymerase alpha3. DNA polymerase alpha1, alpha2 and alpha3 are different molecular forms of the same enzyme, as can be seen by their similar inhibition by N-ethyl-maleimide, heparin and NaCl. However, this new activity, alpha3, is stimulated by dithiothreitol to a greater extent at pH 9.30 than at pH 7.94. The conformational changes induced in DNA polymerase and its increase in activity during infection with SV40 are discussed.

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DNA Polymerase Induced by Simian Virus 40

Cited by 33 publications

References 16 publications

Induction of Cellular Deoxyribonuleic Acid Synthesis by Simian Virus 40

Induction of Cellular Deoxyribonuleic Acid Synthesis by Simian Virus 40

DNA polymerase activity of cultured rheumatoid synovial cells

Fluctuation in activity of the molecular forms of cellular DNA polymerase during infection by SV40

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