DNA polymorphism of FSHR gene and its association with infertility traits in Egyptian buffaloes

Abstract

The objectives of this study were to detect genetic polymorphism in exon 10 of Follicle Stimulating Hormone Receptor gene (FSHR) by using Restriction Fragment Length Polymorphism (RFLP) and direct sequence methods, also to investigate their possible association with infertility traits in Egyptian buffaloes. A total number of 50 female Egyptian buffaloes at Mahallet Mousa experimental farms in Kafr El-sheikh province were investigated. Animals were divided into three groups: normal fertile (20 animals), infertile due to Anestrum (15 animals) and infertile due to repeat breeding (15 animals). Genomic DNA was extracted from blood samples, then PCR was performed with annealing temperatures of 57 ºC to amplify 231 bp of FSHR exon 10 gene. The amplicons were digested with Hin1II (NlaIII) restriction enzyme. The results showed that all the investigated buffaloes were monomorphic and genotyped as A/A genotype. Moreover, the monomorphism of buffaloes FSHR exon 10 gene was confirmed by sequencing three samples from each buffaloes' group. It seems that FSHR gene are highly conserved in the investigated buffalo's population.

Genetic Polymorphism of cyp19 and fshr Genes and Their Association with Ovarian ‎Inactivity and Silent Heat in Egyptian Buffaloes (Bubalus bubalis)‎

“…Regarding to FSH effect in the maintenance of ovarian function, many types of animals have had their fshr gene investigate, including cattle (Houde et al, 1994), sheep (Yarney et al, 1993), horse (Robert et al, 1994) and donkey (Richard et al, 1997). There is lack of literatures concerning the genetic polymorphism linked to reproductive efficiency in buffaloes (Ramadan et al, 2020), consequently, the purpose of this study was to assess genetic polymorphism, RFLPs effects in the cyp19, fshr genes regarding ovarian inactivity and silent heat in Egyptian buffaloes.…”