DNA-probe-target interaction based detection of Brucella melitensis by using surface plasmon resonance

Sikarwar, Bhavna; Singh, Virendra; Sharma, Pushpendra Kumar; Kumar, Adarsh; Thavaselvam, Duraipandian; Boopathi, Mannan; Singh, Beer; Jaiswal, Yogesh K.

doi:10.1016/j.bios.2016.09.063

Cited by 23 publications

(18 citation statements)

References 37 publications

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“…However, the buffer may play a critical role in protein aggregations. Yexuan Mao et al [27] reported the effect of solvents on the aggregation of human islet amyloid polypeptides [11][12][13][14][15][16][17][18][19][20]. They found different behaviors of the peptides aggregation under different buffering systems.…”

Section: Solvent and Concentration Range Considerationsmentioning

confidence: 99%

“…On the other hand, biochemical and biophysical techniques are attractive and developed dramatically to be the first choice in studying biomolecular interactions. Examples include spectroscopic techniques [13][14][15][16][17][18], surface plasma resonance (SPR) [19][20][21], isothermal titration calorimetry (ITC) [22][23][24] and microscale thermophoresis (MST) [25][26][27]. This review will discuss thermophoresis in the characterization of biomolecular interactions.…”

Section: Introductionmentioning

confidence: 99%

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Thermophoresis for characterizing biomolecular interaction

Asmari

Ratih

Alhazmi

et al. 2018

Methods

101

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The study of biomolecular interactions is crucial to get more insight into the biological system. The interactions of protein-protein, protein-nucleic acids, protein-sugars, nucleic acid-nucleic acids and protein-small molecules are supporting therapeutics and technological developments. Recently, the development in a large number of analytical techniques for characterizing biomolecular interactions reflect the promising research investments in this field. In this review, microscale thermophoresis technology (MST) is presented as an analytical technique for characterizing biomolecular interactions. Recent years have seen much progress and several applications established. MST is a powerful technique in quantitation of binding events based on the movement of molecules in microscopic temperature gradient. Simplicity, free solutions analysis, low sample volume, short analysis time, and immobilization free are the MST advantages over other competitive techniques. A wide range of studies in biomolecular interactions have been successfully carried out using MST, which tend to the versatility of the technique to use in screening binding events in order to save time, cost and obtained high data quality.

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Section: Solvent and Concentration Range Considerationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Thermophoresis for characterizing biomolecular interaction

Asmari

Ratih

Alhazmi

et al. 2018

Methods

101

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“…Both families of techniques show advantages and drawbacks. Label-free techniques, such as surface plasmon resonance (SPR), are generally used to investigate in real-time the kinetics of surface-binding events, providing quantitative information on specific biomolecular binding constants (Dey et al, 2012;Guo, 2012;Sikarwar et al, 2017;Ulber et al, 2003). While the latter is a unique and valuable feature of label-free methods, resolution is usually lower compared to the fluorescence-based methods (Mathias et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices

Rizzo

Alvaro

Danz

et al. 2018

Sensors and Actuators B: Chemical

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We report on the detection of an angiogenic molecule Vascular Endothelial Growth Factor (VEGF) in different biological matrices by means of a new integrated biosensing platform exploiting the properties of Bloch surface waves. The new platform takes advantage of a tandem configuration, in which both label-free and enhanced fluorescence detection are implemented. Specifically designed one dimensional photonic crystals were deposited directly on disposable and low cost plastic biochips. A direct sandwich immunoassay was used to detect VEGF in buffer, cell culture supernatant and human plasma at low concentration (ng/mL). The platform enabled the detection of VEGF in all three matrices with high resolution, fast turnaround time (30 min) and in close agreement with the results of enzyme linked immunosorbent assays

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“…Sikarwar et al prepared an SPR sensor using 4-mercaptobenzoic acid-modified gold for Brucella melitensis detection using its complementary DNA targets with two different probes of the IS711 gene [ 80 ]. They performed kinetics and thermodynamic analysis, and the results reflected that complementary DNA targets and Probe 1 are a more effective interaction than Probe 2 ( Figure 3 ).…”

Section: Monitoring Biological and Chemical Threats Agentsmentioning

confidence: 99%

“… Surface plasmon resonance (SPR) sensor responses for different dilutions’ (1:12,800 (a), 1:6400 (b), 1:3200 (c), 1:1600 (d), 1:800 (e), 1:400 (f), 1:200 (g) and 1:100 (h)) interaction with the DNA target for Probe 1 ( A ) and 2 ( B ). Republished with permission from [ 80 ]; permission conveyed through Copyright Clearance Center, Inc. …”

Section: Figurementioning

confidence: 99%

Plasmonic Sensors for Monitoring Biological and Chemical Threat Agents

2020

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Sensors are excellent options owing to their ability to figure out a large number of problems and challenges in several areas, including homeland security, defense, medicine, pharmacology, industry, environment, agriculture, food safety, and so on. Plasmonic sensors are used as detection devices that have important properties, such as rapid recognition, real-time analysis, no need labels, sensitive and selective sensing, portability, and, more importantly, simplicity in identifying target analytes. This review summarizes the state-of-art molecular recognition of biological and chemical threat agents. For this purpose, the principle of the plasmonic sensor is briefly explained and then the use of plasmonic sensors in the monitoring of a broad range of biological and chemical threat agents is extensively discussed with different types of threats according to the latest literature. A conclusion and future perspectives are added at the end of the review.

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DNA-probe-target interaction based detection of Brucella melitensis by using surface plasmon resonance

Cited by 23 publications

References 37 publications

Thermophoresis for characterizing biomolecular interaction

Thermophoresis for characterizing biomolecular interaction

Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices

Plasmonic Sensors for Monitoring Biological and Chemical Threat Agents

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