DNA Profiling and forensic dentistry – A review of the recent concepts and trends

Manjunath, BC; Chandrashekar, BS; Mahesh, Melkundi; Rani, Reshma

doi:10.1016/j.jflm.2011.02.005

Cited by 48 publications

(66 citation statements)

References 85 publications

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“…The durability of the human teeth plays an important part resisting to natural and PM alterations induced by men (Vieira et al, 2010;Manjunath et al, 2011), such as putrefaction and carbonization, respectively. Cadaveric alterations evolved following the current changes in criminality, which includes more complex mechanisms compared to decades ago.…”

Section: Resultsmentioning

confidence: 99%

Testing the Extraction of DNA from Human Teeth Exposed to Different Chemical Solutions

Damascena

Filho

Souza

et al. 2017

Int. J. Odontostomat.

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ABSTRACT:The aim of this study was to evaluate the extraction of dental DNA exposed to different chemical solutions. The sample was composed by 15 subjects, from which 5 samples of oral mucosal cells (reference population) and 15 teeth (experimental population) were collected. The experimental population was divided in three equal parts, which were exposed to different chemical solutions, namely hydrochloric acid (HCl) at 37 %, formaldehyde (CH 2 O) at 10 % and sodium hypochlorite (NaOCl) at 2.5 %. The DNA from the oral mucosa was extracted using organic method, while the dental DNA was extracted using the AFDIL method, including amplification by PCR and sequencing through capillary electrophoresis. The DNA exposed to hydrochloric acid dissolved, lacking extraction. The exposure of teeth to formaldehyde and sodium hypochlorite did not interfere in the extraction of DNA, once the amplification was visible in both experimental populations. The present outcomes demonstrated that DNA extraction may be limited under exposure to chemical solutions.

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Section: Resultsmentioning

confidence: 99%

Testing the Extraction of DNA from Human Teeth Exposed to Different Chemical Solutions

Damascena

Filho

Souza

et al. 2017

Int. J. Odontostomat.

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“…The interesting fact is that the tooth is the hardest structure withstanding temperatures even as high as 1600°C and thereby resisting potentially destruction and decomposition for considerable periods after death. [13] The next dimension of forensic is reflected by autopsy which is upgraded by Virtopsy. Respecting deeply the human body even after death, in exploring, the cause of death is Virtopsy -a recent advance outshining conventional autopsy.…”

Section: Forensic Identification and Virtopsymentioning

confidence: 99%

Interdisciplinary role of oral physician in stomatognathic diseases

Jeelani¹,

Duraiselvi²,

Priyadharscini³

2017

JCRI

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Diagnosis is a pivotal step against any pathology. The role of oral physician in the management of stomatognathic diseases has always been a challenging step both in terms of diagnosis and therapeutics. The scope of the oral physician is diverse. This paper is just a tip of the iceberg reflecting lateral and integrated responsibilities of the specialty in the management of oral and systemic diseases such as temporomandibular disorders (TMDs), cancer, behavioral disorders, human immunodeficiency virus (HIV), and forensics. The significance of brain pacemakers in the management of refractory depression as a part of neuropsychopharmacological integration, inflammation-based therapeutic modality such as prolotherapy in the treatment of TMD, deoxyribonucleic acid (DNA) methylation as part of epigenetics in the management of cancer, virtual autopsy using imaging in forensic identification, and phytochemicals as supportive adjuvant for HIV has been dealt as part of this paper. The paper portrays the silent guiding role of an oral physician in the interdisciplinary approach with respect to stomatognathic diseases.

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“…Medical statistics from the US show that approximately 15% of all couples of reproductive age are unable to conceive naturally (Fernandez et al 1991). 3 Approximately 10% of couples at child bearing ages suffered from some kind of infertility and about half of these cases are because of male factors (de Kretser and Baker 1999), 2 which recently became a hot issue in the relevant studies. The Y chromosome microdeletions are the most common genetic causes of male infertility due to spermatogenesis failure and have been reported in 2.7 to 55.5% of infertile men (Krausz et al 2000) 4 and (Simoni et al 1999) 5 The frequency of Y chromosome microdeletions increases with the severity of spermatogenesis defect (Krausz and McElreavey, 1999).…”

Section: Introductionmentioning

confidence: 99%

Utilization of Dental Pulp DNA as Diagnostic Molecular Marker for Fertility Detection in Men

Eid

Taha²,

Al-Ahmari³

et al. 2013

The Journal of Contemporary Dental Practice

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Recent advances in DNA technology have revolutionized forensic identification procedures. Teeth dentin and pulp are rich sources of DNA material, which can be successfully extracted and it provides us with valuable information on individuals, systemic health including fertility status. Aim of study:The aim of this study was to use DNA material extracted from human teeth pulp for detection of fertility status of men. Materials and methods:Twenty extracted premolar teeth of systemic disease free male Saudi individuals (45 years average age) were collected; eight of them were infertile while others were fertile and were used as control group. This information was concealed until the PCR analysis was performed. The results of recorded patient information was matched with the results of the DNA analysis. Results:Results showed that the gene (sY83) an important gene of AZFa region in Y chromosome is important for male fertility. It was later evident that the infertile patients suffered from azoospermia, and that information is completely matched with our results. Conclusion:Using DNA extracted from dental pulp can be used successfully in determining fertility status of human which may help in an accurate personal identification specially in extreme circumstances.

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DNA Profiling and forensic dentistry – A review of the recent concepts and trends

Cited by 48 publications

References 85 publications

Testing the Extraction of DNA from Human Teeth Exposed to Different Chemical Solutions

Testing the Extraction of DNA from Human Teeth Exposed to Different Chemical Solutions

Interdisciplinary role of oral physician in stomatognathic diseases

Utilization of Dental Pulp DNA as Diagnostic Molecular Marker for Fertility Detection in Men

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