DNA profiling in peripheral blood, buccal swabs, hair follicles and semen from a patient following allogeneic hematopoietic stem cells transplantation

Abstract

Allogeneic peripheral blood stem cells transplantation (allo-PBSCT) or allogeneic bone marrow transplantation (allo-BMT) have been widely used to treat patients exhibiting certain severe illnesses. However, previous studies have shown that the biological materials of allo-PBSCT or allo-BMT recipients may not constitute credible materials for personal identification. In the present study, four types of commonly used samples were collected from a male individual following gender-matched allo-BMT. Autosomal short tandem repeat (STR) and Y-STR markers analysis, based on polymerase chain reaction, were used to evaluate the chimerism status. The results showed that the blood sample were all donor type, the buccal swab sample were mixed chimerism, and the sperm and hair follicle samples maintained a recipient origin of 100%. In conclusion, identical results were obtained by the two methods and it was confirmed that DNA extracted from hair follicles and sperm can be used as a reference for the pre-transplant genotype DNA profile of the recipient in the gender-match allo-BMT or -PBSCT.

Chimerism Monitoring Techniques after Hematopoietic Stem Cell Transplantation: An Overview of the Last 15 Years of Innovations

“…Moreover, they also stated that if only one between the donor or the recipient is available for DNA extraction, STR variability (which implies a low probability that donor and recipient share the same STR alleles) allows for the identification of the amount of recipient cells after allogeneic HSCT [ 34 ]. Li et al [ 51 ] pointed out that, after the transplantation, the collection of recipient biological material through buccal swab may represent a problem given that a buccal swab after allogeneic HSCT can manifest MC. However, for Navarro-Bailón et al [ 34 ], when donor STR profile is available, it is possible to ignore those peaks of the recipient’s buccal swab electropherogram corresponding to donor alleles, permitting the ready identification of those of the recipient.…”

DNA profiling in blood, buccal swabs, and hair follicles of transplantation patients

“…Forensic scientists use DNA from various biological samples found at crime scenes to identify criminal suspects by DNA profiling. Different types of DNA samples could be used for DNA analysis according to the hypothesis that all the cells in the human body have an identical DNA profile (18). As in every area of forensic science, it is also important to gain accurate and complete genetic information as well.…”

Postmortem Detection of Liver Chimerism after Allogeneic Hematopoietic Stem Cell Transplantation in Adult Male Mice

“…Allogeneic hematopoietic stem cells transplantation (allo-HSCT) has been widely used to treat patients with several illnesses, but the biological materials of those recipients may cause problems in personal identification due to what is called "chimerism" (Li et al, 2014). Chimerism is defined in transplantation medicine as the coexistence ofcells of, both, donor and recipient origin (Ten Hove et al, 2007).…”

“…Chimerism is defined in transplantation medicine as the coexistence ofcells of, both, donor and recipient origin (Ten Hove et al, 2007). In previous studies, blood, hair samples and buccal swabs are studied as sources of DNA profiling after hematopoietic stem cell transplantation (Hong et al, 2007;Zhou et al, 2011;Li et al, 2014;Chaudhary et al, 2015;Santurtún et al, 2017) The partial chimerism is detectable predominantly in the P hematopoietic system developed by transfusion or transplantation of allogenic hematopoietic stem cells (Milde et al, 1999). This experimental work aims at studying the possibility of postmortem detection in DNA chimerism in liver tissue, after allogeneic hematopoietic stem cell transplantation.…”