DNA profiling of commercial chilli pepper (Capsicum annuum L.) varieties using random amplified polymorphic DNA (RAPD) markers

Bylla, Prasad; Khan, Rohela Gulab; Radha, Toutreddy; Ravi, C. S.; Venkataiah, P.; Subhash, K.; Reuben, T. Christopher

doi:10.5897/ajb2012.3017

Cited by 14 publications

(7 citation statements)

References 23 publications

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“…The similarity of the genome sequences of some of these mutants indicates the stability of the mutants. The same thing was shown by Prasad et al (2013) who found that there were variations in the RAPD's band profile associated with morphological variations of chili peppers collected from Andhra Pradesh India, while the similarity of the band profile was related to the similarity of several morphological characters such as flower color, fruit size, and fruit orientation. Rêgo et al (2011) also found that RAPD markers were effective in detecting the genetic diversity among 29 hot pepper accessions from Brazil.…”

Section: Polymorphism Of Mutant G7/01 and Initial Line Based On Rapd ...supporting

confidence: 64%

Confirmation of mutation and genetic stability of the M4 generation of chili pepper’s (Capsicum frutescens L.) Ethyl Methane Sulfonate (EMS) mutant based on morphological, physiological and molecular characters

Arumingtyas

ATIATURROCHMAH²,

Kusnadi³

2023

Biodiversitas

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Abstract. Arumingtyas EL, Atiaturrochmah, Kusnadi J. 2023. Confirmation of mutation and genetic stability of the M4 generation of chili pepper’s (Capsicum frutescens L.) Ethyl Methane Sulfonate (EMS) mutant based on morphological, physiological and molecular characters. Biodiversitas 24: 531-538. The initial generation of Ethyl Methane Sulfonate (EMS) random mutations usually still shows high variation due to allele segregation. This research aimed to confirm the genetic differences between the M4 generation of chili pepper (Capsicum frutescens L.) mutant resulted from EMS mutations (G7/01) and the initial line (G7), and the stability of the mutant based on morphological, physiological, and molecular characters. The morphological characters and the capsaicinoid content of the mutants G7/01 with the initial line (G7) were compared. The capsaicinoid content and fruit spiciness were measured by spectrophotometer ? 280 nm. Molecular characterization was conducted using Random Amplified Polymorphic DNA (RAPD) genetic markers. Based on the morphological characters, the G7/01 mutants have some superior properties compared to the initial line G7. All the G7/01 mutant plants contain higher capsaicinoid compounds than the initial line plants. The dendrogram developed based on RAPD profile showed that all the mutant plants were positioned apart from the initial line plant, suggesting that there are some genomic changes in the mutants compared to the initial line plants. All the mutant plants, except T1, showed insignificant variation in morphological characteristic, capsaicin content, and RAPD profile, indicating genetic stability.

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Section: Polymorphism Of Mutant G7/01 and Initial Line Based On Rapd ...supporting

confidence: 64%

Confirmation of mutation and genetic stability of the M4 generation of chili pepper’s (Capsicum frutescens L.) Ethyl Methane Sulfonate (EMS) mutant based on morphological, physiological and molecular characters

Arumingtyas

ATIATURROCHMAH²,

Kusnadi³

2023

Biodiversitas

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“…Genomic DNA was isolated from acclimated plantlets of R. tetraphylla (seven plants were selected randomly, four from leaf callus based and three from stem callus based regenerants) from field along with the mother plant. Fresh leaf tissue (500 mg) of both mother plant and in vitro regenerated plants was used as raw material for DNA isolation by following modified cetyl trimethyl ammonium bromide (CTAB) method [28, 29]. The quality parameters of DNA were checked by spectrometric and electrophoretic methods.…”

Section: Methodsmentioning

confidence: 99%

Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

Rohela

Jogam

Bylla

et al. 2019

BioMed Research International

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Rauwolfia tetraphylla L. is an important medicinal plant species which is well known for its pharmaceutically important alkaloids. In the present study, we are reporting about its conservation by in vitro clonal multiplication through the standardized protocol of indirect regeneration by using leaf and stem based callus and assessment of genetic fidelity of acclimated plantlets by start codon targeted (SCoT), inter simple sequence repeats (ISSR), and randomly amplified polymorphic DNA (RAPD) marker based analysis. Initially friable callus was induced in maximum amounts (378.7, 323.8, and 412.8 in mg) from leaf, root, and stem explants on Murashige and Skoog (MS) media supplemented with 5.0 mg/L, 3.0 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D) and 5.0 mg/L of naphthalene acetic acid (NAA), respectively. Shoot regeneration with the maximum number of shoot buds (25 and 20) was obtained from leaf and stem calluses on MS media supplemented with TDZ (0.25 mg/L) + BAP (2 mg/L). The regenerated shoots were rooted successfully with maximum rooting percentage of 98.0 on full strength MS media amended with IAA (1.0 mg/L) and IBA (1.0 mg/L). The regenerated plantlets were hardened using 2:1 ratio of sterile garden soil and sand, followed by acclimatization in field conditions with 86% of survival. SCoT, ISSR, and RAPD primers based polymerase chain reaction (PCR) analysis was carried out to check possible genetic variations in micro propagated plants in comparison with mother plant. Among the ten SCoT (S), ISSR (R), and RAPD (OPA) primers used, S2, R10, and OPA3 has given good amplification with scorable DNA bands. The results revealed that the regenerated plants did not have any polymorphism with mother plant. Hence, the in vitro regenerated R. tetraphylla plantlets were confirmed as true-to-type.

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“…RAPD primers were earlier reported for both DNA profiling and genetic fidelity studies in several plant species. Prasad et al 26 has reported DNA profiling of chilli peppers by using RAPD primers. Similarly, RAPD primers were used for analyzing genetic fidelity of Populus deltoides 27 , Morus alba 28 , Swertia chirayita 29 .…”

Section: Results and Discussion:-mentioning

confidence: 99%

In Vitro Micropropagation of Stachytarpheta Jamaicensis L.VAHL., an Ethnomedicinal Plant and Confirmation of Genetic Fidelity of the Plantlets Using Rapd Markers.

Korra¹,

Prasad²,

Rohela³

et al. 2017

IJAR

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DNA profiling of commercial chilli pepper (Capsicum annuum L.) varieties using random amplified polymorphic DNA (RAPD) markers

Abstract: In the present study, genetic variability in 10 commercial chilli pepper varieties viz. Gemini,

Cited by 14 publications

References 23 publications

Confirmation of mutation and genetic stability of the M4 generation of chili pepper’s (Capsicum frutescens L.) Ethyl Methane Sulfonate (EMS) mutant based on morphological, physiological and molecular characters

Confirmation of mutation and genetic stability of the M4 generation of chili pepper’s (Capsicum frutescens L.) Ethyl Methane Sulfonate (EMS) mutant based on morphological, physiological and molecular characters

Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

In Vitro Micropropagation of Stachytarpheta Jamaicensis L.VAHL., an Ethnomedicinal Plant and Confirmation of Genetic Fidelity of the Plantlets Using Rapd Markers.

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