In Vitro Micropropagation of Stachytarpheta Jamaicensis L.VAHL., an Ethnomedicinal Plant and Confirmation of Genetic Fidelity of the Plantlets Using Rapd Markers.

Korra, Rajender; Prasad, B. N. Maruthi; Rohela, GulabKhan; Pendli, Sreenu; Reuben, T. Christopher

doi:10.21474/ijar01/4862

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…Sujatha et al (2013) obtained maximum number of plantlets in sponge gourd (Luffacy lindrica) by using leaf and nodal explants at 1.5 mg/L concentration of BAP. Similarly on individually supplemented BAP (Rohela et al, 2018b) and in combinations of BAP with TDZ (Thiadiazuron) (Rohela et al, 2013;Rohela et al, 2015;Rohela et al, 2016c;Korra et al, 2017;Rohela et al, 2018a) of multiple shoots were regenerated from nodal explants, shoot tip explants, leaf and stem based calluses of Rauwolfia tetraphylla and Morus Sps.…”

Section: Discussionmentioning

confidence: 99%

RAPID ONE STEP PROTOCOL FOR THE in vitro MICRO PROPAGATION OF Morus multicaulis VAR. GOSHOERAMI, AN ELITE MULBERRY VARIETY OF TEMPERATE REGION

Rohela¹,

Shabnam²,

Shukla³

et al. 2018

JEBAS

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Morus multicaulis cv. Goshoerami is the leading mulberry variety for silkworm rearing under temperate climatic conditions of Jammu and Kashmir, India. However, the propagation of this popular mulberry variety has always remained a point of contention due to its poor rooting response through stem cuttings. It normally takes 4 to 5 years for raising the saplings of this variety through conventional root grafting techniques. Therefore, for quick propagation of this poor rooting popular mulberry variety, a one step in vitro protocol was developed by culturing nodal explants from 2 year old plants on Murashige & Skoog (MS) media supplemented with individual as well as combination of phytohormones. The maximum shoot bud proliferation (6.3± 0.71 in cm) and rooting (14.7± 0.53 in cm) was observed when nodal explants were cultured on the combinational media of BAP (1 mg/L) and IBA (1 mg/L) after 14 days of culture. These in vitro raised plantlets were hardened by using the sterile soil and vermiculite in 2:1 ratio. Only 25 days were required for the micro propagation and hardening of raised plantlets of Goshoerami through this single step protocol. The hardened plantlets were successfully established in the field with 83% survival rate. The developed one step protocol can be used efficiently for the mass propagation of this elite mulberry variety throughout the year with in short span of 25 days.

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Section: Discussionmentioning

confidence: 99%

RAPID ONE STEP PROTOCOL FOR THE in vitro MICRO PROPAGATION OF Morus multicaulis VAR. GOSHOERAMI, AN ELITE MULBERRY VARIETY OF TEMPERATE REGION

Rohela¹,

Shabnam²,

Shukla³

et al. 2018

JEBAS

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“…To quantify the amount of DNA isolated from the 1 Gram of Leaf sample of 5 Rauwolfia species, we have used the spectrophotometer measurements of the amount of ultraviolet irradiation absorbed by the bases at 260 and 280 nm (Korra et al, 2017).…”

Section: Quantification and Quality Analysis Of Isolated Dnamentioning

confidence: 99%

ISSR marker based DNA profiling studies in Rauwolfia species

et al. 2018

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In this present study, a protocol was developed for the DNA profiling of medicinally important Rauwolfia species (05 species) by ISSR-PCR method. Initially genomic DNA was isolated from the fresh leaves (1 gram) of 24 months old Rauwolfia tetraphylla, R.serpentina, R.pentaphylla, R.vomitaria and R.micrantha. The content of genomic DNA isolated from 1 gram of fresh leaves of these five species ranges from 1.6 to 3.4 µg. Out of six inter simple sequence repeats (ISSR) primers used, HY3 primer has given the good amplification with distinct bands which were reproducible. The range of DNA bands produced by the HY3 primer were between 300 bp to 1500 bp among the five species. A dendrogram was constructed on the basis of similarity matrix data by unweighted pair group method with arithmetic average (UPGMA) cluster analysis using indigenous software. Average genetic similarity generated by HY3 has revealed 38% similarity between species of Sub cluster-A and species of Sub cluster-B which is consistent with their geographical distribution i.e. Sub cluster-A species R.tetraphylla and R.serpentina inhabit peninsular India, Sub cluster- B species R.micrantha and R. vomitoria inhabit the Himalayan foot hills. The developed protocol of DNA profiling in Rauwolfia species by ISSR-PCR method could be used efficiently for the differentiation and identification of the Rauwolfia species at molecular level.

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High frequency in vitro plantlet regeneration in Solanum trilobatum L., an important ethno-medicinal plant and confirmation of genetic fidelity of R1 plantlets by using ISSR and RAPD markers

Pendli

Rohela

Jogam

et al. 2019

Vegetos

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In Vitro Micropropagation of Stachytarpheta Jamaicensis L.VAHL., an Ethnomedicinal Plant and Confirmation of Genetic Fidelity of the Plantlets Using Rapd Markers.

Cited by 4 publications

References 16 publications

RAPID ONE STEP PROTOCOL FOR THE in vitro MICRO PROPAGATION OF Morus multicaulis VAR. GOSHOERAMI, AN ELITE MULBERRY VARIETY OF TEMPERATE REGION

RAPID ONE STEP PROTOCOL FOR THE in vitro MICRO PROPAGATION OF Morus multicaulis VAR. GOSHOERAMI, AN ELITE MULBERRY VARIETY OF TEMPERATE REGION

ISSR marker based DNA profiling studies in Rauwolfia species

High frequency in vitro plantlet regeneration in Solanum trilobatum L., an important ethno-medicinal plant and confirmation of genetic fidelity of R1 plantlets by using ISSR and RAPD markers

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