DNA‐Programmed Stem Cell Niches via Orthogonal Extracellular Vesicle–Cell Communications

Wang, Kaizhe; Wei, Yuhan; Xie, Xiaodong; Li, Qian; Liu, Xiaoguo; Wang, Lihua; Wu, Ji; Fan, Chunhai

doi:10.1002/adma.202302323

Cited by 5 publications

(2 citation statements)

References 71 publications

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“…Compared to synthetic nanoparticles, EVs as naturally occurring vesicles possess evolutionary selection advantages, rendering them attractive alternative vehicles for cargo delivery. EVs have excellent biocompatibility, desired nanoscopic size, low immunogenicity, and the ability to overcome biological barriers. , The remarkable features of EVs enable them to deliver endogenous functional components as well as to carry exogenous drugs, driving the development of EV-based therapies and bioengineering. − However, the full potential of EVs has not yet been realized, mainly due to their poor controllability and limited cellular delivery capacity.…”

Section: Introductionmentioning

confidence: 99%

“… 2 , 3 The remarkable features of EVs enable them to deliver endogenous functional components as well as to carry exogenous drugs, driving the development of EV-based therapies and bioengineering. 4 − 6 However, the full potential of EVs has not yet been realized, mainly due to their poor controllability and limited cellular delivery capacity.…”

Section: Introductionmentioning

confidence: 99%

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Extracellular Vesicle Spherical Nucleic Acids

Chen,

Ding,

et al. 2024

JACS Au

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Extracellular vesicles (EVs) are naturally occurring vesicles secreted by cells that can transport cargo between cells, making them promising bioactive nanomaterials. However, due to the complex and heterogeneous biological characteristics, a method for robust EV manipulation and efficient EV delivery is still lacking. Here, we developed a novel class of extracellular vesicle spherical nucleic acid (EV-SNA) nanostructures with scalability, programmability, and efficient cellular delivery. EV-SNA was constructed through the simple hydrophobic coassembly of natural EVs with cholesterol-modified oligonucleotides and can be stable for 1 month at room temperature. Based on programmable nucleic acid shells, EV-SNA can respond to AND logic gates to achieve vesicle assembly manipulation. Importantly, EV-SNA can be constructed from a wide range of biological sources EV, enhancing cellular delivery capability by nearly 10−20 times. Compared to artificial liposomal SNA, endogenous EV-SNA exhibited better biocompatibility and more effective delivery of antisense oligonucleotides in hard-to-transfect primary stem cells. Additionally, EV-SNA can deliver functional EVs for immune regulation. As a novel material form, EV-SNA may provide a modular and programmable framework paradigm for EV-based applications in drug delivery, disease treatment, nanovaccines, and other fields.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Extracellular Vesicle Spherical Nucleic Acids

Chen,

Ding,

et al. 2024

JACS Au

Self Cite

View full text Add to dashboard Cite

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DNA Nanomaterial‐Empowered Surface Engineering of Extracellular Vesicles

Yao,

He,

Wei

et al. 2023

Advanced Materials

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Extracellular vesicles (EVs) are cell‐secreted biological nanoparticles that are critical mediators of intercellular communication. They contain diverse bioactive components, which are promising diagnostic biomarkers and therapeutic agents. Their nanosized membrane‐bound structures and innate ability to transport functional cargo across major biological barriers make them promising candidates as drug delivery vehicles. However, the complex biology and heterogeneity of EVs pose significant challenges for their controlled and actionable applications in diagnostics and therapeutics. Recently, DNA molecules with high biocompatibility emerge as excellent functional blocks for surface engineering of EVs. The robust Watson–Crick base pairing of DNA molecules and the resulting programmable DNA nanomaterials provide the EV surface with precise structural customization and adjustable physical and chemical properties, creating unprecedented opportunities for EV biomedical applications. This review focuses on the recent advances in the utilization of programmable DNA to engineer EV surfaces. The biology, function, and biomedical applications of EVs are summarized and the state‐of‐the‐art achievements in EV isolation, analysis, and delivery based on DNA nanomaterials are introduced. Finally, the challenges and new frontiers in EV engineering are discussed.

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Controllable Modulation of Third‐Order NLO Properties by Polymer‐Modified MOFs Core@Shell Nanoarchitecture

Huang,

Zhou,

Cui

et al. 2024

Advanced Optical Materials

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The delocalization and rearrangement of π electron clouds in space is the crucial source of regulating nonlinear optical (NLO) performance. Herein, a core‐shell metal organic framework (MOF)‐heterojunction is constructed to achieve the effect of tuning NLO properties by promoting charge transfer through multiple interactions between heterogeneous interfaces. [Cd(L)(4,4′‐bpy)(H2O)]n (Cd‐MOF) is selected to coat on polyaniline (PANI) surface to form PANI@Cd‐MOF. The constructed heterojunction displays an enhanced reverse saturation absorption (RSA) signal compared with Cd‐MOF, and it should be noted that the third‐order NLO refractive behavior changes from the self‐defocusing of Cd‐MOF to the self‐focusing of PANI@Cd‐MOF. It is proposed that high‐speed electronic communication bridges established via the rich hydrogen bond network (O… H‐N) and π–π interactions between heterogeneous interfaces can effectively modify the π electron cloud density that finally regulates the NLO properties. This work is believed to provide new avenues and insights for the design of third‐order NLO materials.

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DNA‐Programmed Stem Cell Niches via Orthogonal Extracellular Vesicle–Cell Communications

Cited by 5 publications

References 71 publications

Extracellular Vesicle Spherical Nucleic Acids

Extracellular Vesicle Spherical Nucleic Acids

DNA Nanomaterial‐Empowered Surface Engineering of Extracellular Vesicles

Controllable Modulation of Third‐Order NLO Properties by Polymer‐Modified MOFs Core@Shell Nanoarchitecture

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