DNA repair choice defines a common pathway for recruitment of chromatin regulators

Bennett, Gwendolyn; Papamichos‐Chronakis, Manolis; Peterson, Craig L.

doi:10.1038/ncomms3084

Cited by 88 publications

(97 citation statements)

References 49 publications

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“…4) compared with wt mice. Up-regulated miRNA in 3 0 RR-deficient mice have hundreds of ARTICLE predicted targets, including Atm, Ssb1, Ino80, Exo1, Smarcd1, Dclre1b and Atmin, which have been shown to regulate DSB resection and homologous recombination [33][34][35][36][37][38] , and histone modifying enzymes Hdac9 and Esco1. Interestingly, HDAC9 is required for DSB reparation by homologous reparation 39 , and its deletion affects antibody secretion 40 .…”

Section: ′Rr-deficientmentioning

confidence: 99%

Elucidation of IgH 3′ region regulatory role during class switch recombination via germline deletion

et al. 2015

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In mature B cells, class switch recombination (CSR) replaces the expressed constant Cm gene with a downstream C H gene. How the four transcriptional enhancers of the IgH 3 0 regulatory region (3 0 RR) control CSR remains an open question. We have investigated IgG 1 CSR in 3 0 RR-deficient mice. Here we show that the 3 0 RR enhancers target the S g1 acceptor region (and poorly the S m donor region) by acting on epigenetic marks, germline transcription, paused RNA Pol II recruitment, R loop formation, AID targeting and double-strand break generation. In contrast, location and diversity of S m -S g1 junctions are not affected by deletion of the 3 0 RR enhancers. Thus, the 3 0 RR controls the first steps of CSR by priming the S acceptor region but is not implicated in the choice of the end-joining pathway.

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Section: ′Rr-deficientmentioning

confidence: 99%

Elucidation of IgH 3′ region regulatory role during class switch recombination via germline deletion

et al. 2015

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“…These results suggest that, although Tel9R and Tel13R-f share overlapping nuclear territories (Agmon et al, 2013), the proximity of homologous sequences is not sufficient for homologous repair to take place efficiently in the presence of latrunculin. The drop in the efficiency of homologous recombination in the presence of latrunculin A remains to be further characterized and could result from several mechanisms that are not mutually exclusive -the established role for actin in the INO80 complex (Agmon et al, 2013;Bennett et al, 2013;Horigome et al, 2014;Kapoor et al, 2013) -or a possible role in facilitating homology search through chromosome jiggling.…”

Section: -Gfp-labeled Cells (Wt) That Had Been Treated With Dmso or 3mentioning

confidence: 99%

“…We found that actin-dependent chromosome movements affect homologous recombination repair efficiency. This repair mechanism requires the recruitment of INO80, SWR-C, NuA4, SWI/SNF and RSC to the DSBs during S phase (Bennett et al, 2013). Interestingly, the INO80, SWR-C and NuA4 complexes contain actin in both yeast and mammals (for review, see Kapoor and Shen, 2014).…”

Section: Functional Consequencesmentioning

confidence: 99%

“…Nuclei displacements were corrected, and only one-third of the curve was taken into consideration to fit α and C (subdiffusion coefficient). Shaded area surrounding lines ±s.e.m.. (Bennett et al, 2013). We therefore tested the efficiency of the homologous recombination repair pathway in the absence of filamentous actin.…”

Section: -Gfp-labeled Cells (Wt) That Had Been Treated With Dmso or 3mentioning

confidence: 99%

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Evidence for actin dual role in regulating chromosome organization and dynamics in yeast

Spichal

Brion

Herbert

et al. 2016

Journal of Cell Science

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Eukaryotic chromosomes undergo movements that are involved in the regulation of functional processes such as DNA repair. To better understand the origin of these movements, we used fluorescence microscopy, image analysis and chromosome conformation capture to quantify the actin contribution to chromosome movements and interactions in budding yeast. We show that both the cytoskeletal and nuclear actin drive local chromosome movements, independently of Csm4, a putative LINC protein. Inhibition of actin polymerization reduces subtelomere dynamics, resulting in more confined territories and enrichment in subtelomeric contacts. Artificial tethering of actin to nuclear pores increased both nuclear pore complex (NPC) and subtelomere motion. Chromosome loci that were positioned away from telomeres exhibited reduced motion in the presence of an actin polymerization inhibitor but were unaffected by the lack of Csm4. We further show that actin was required for locus mobility that was induced by targeting the chromatin-remodeling protein Ino80. Correlated with this, DNA repair by homologous recombination was less efficient. Overall, interphase chromosome dynamics are modulated by the additive effects of cytoskeletal actin through forces mediated by the nuclear envelope and nuclear actin, probably through the function of actin in chromatin-remodeling complexes.

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“…Wu and colleagues showed that knockdown models of human INO80, or of its binding partner YY1, increased cellular sensitivity toward DNAdamaging agents, and in their study they highlighted the essentiality of both INO80 and YY1 for HR repair (Wu, S. et al, 2007). However, a recent report indicated that H2A phosphorylation is dispensable for recruitment of the chromatin remodelers INO80 and SNF6 (a component of SWI/SNF) to DSB sites in yeast (Bennett et al, 2013), suggesting that the interaction with γ-H2AX may not be essential for directing the accumulation of histone remodelers at DSB damage sites.…”

Section: Introductionmentioning

confidence: 99%

Chromatin modification and NBS1: their relationship in DNA double-strand break repair

2015

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The importance of chromatin modification, including histone modification and chromatin remodeling, for DNA double-strand break (DSB) repair, as well as transcription and replication, has been elucidated. Phosphorylation of H2AX to γ-H2AX is one of the first responses following DSB detection, and this histone modification is important for the DSB damage response by triggering several events, including the accumulation of DNA damage response-related proteins and subsequent homologous recombination (HR) repair. The roles of other histone modifications such as acetylation, methylation and ubiquitination have also been recently clarified, particularly in the context of HR repair. NBS1 is a multifunctional protein that is involved in various DNA damage responses. Its recently identified binding partner RNF20 is an E3 ubiquitin ligase that facilitates the monoubiquitination of histone H2B, a process that is crucial for recruitment of the chromatin remodeler SNF2h to DSB damage sites. Evidence suggests that SNF2h functions in HR repair, probably through regulation of end-resection. Moreover, several recent reports have indicated that SNF2h can function in HR repair pathways as a histone remodeler and that other known histone remodelers can also participate in DSB damage responses. On the other hand, information about the roles of such chromatin modifications and NBS1 in non-homologous end joining (NHEJ) repair of DSBs and stalled fork-related damage responses is very limited; therefore, these aspects and processes need to be further studied to advance our understanding of the mechanisms and molecular players involved.

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DNA repair choice defines a common pathway for recruitment of chromatin regulators

Cited by 88 publications

References 49 publications

Elucidation of IgH 3′ region regulatory role during class switch recombination via germline deletion

Elucidation of IgH 3′ region regulatory role during class switch recombination via germline deletion

Evidence for actin dual role in regulating chromosome organization and dynamics in yeast

Chromatin modification and NBS1: their relationship in DNA double-strand break repair

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