2012
DOI: 10.1371/journal.pone.0030541
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DNA Repair in Human Pluripotent Stem Cells Is Distinct from That in Non-Pluripotent Human Cells

Abstract: The potential for human disease treatment using human pluripotent stem cells, including embryonic stem cells and induced pluripotent stem cells (iPSCs), also carries the risk of added genomic instability. Genomic instability is most often linked to DNA repair deficiencies, which indicates that screening/characterization of possible repair deficiencies in pluripotent human stem cells should be a necessary step prior to their clinical and research use. In this study, a comparison of DNA repair pathways in plurip… Show more

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Cited by 52 publications
(56 citation statements)
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“…Normally, cells of early mammalian embryos and embryonic stem cells cycle rapidly, with significantly shortened G1 phase of the cell cycle. The G1/S phase checkpoint is activated in the presence of DNA damage and is associated with decreasing the risk for transmitting mutations to the cell's progeny [150][151][152]. The strictness of the control checkpoint in G1 is relaxed in embryonic cells compared to the requirements of G1/S transition in somatic cells [153].…”
Section: Increasing the Propensity For Differentiation Along Multiplementioning
confidence: 99%
See 1 more Smart Citation
“…Normally, cells of early mammalian embryos and embryonic stem cells cycle rapidly, with significantly shortened G1 phase of the cell cycle. The G1/S phase checkpoint is activated in the presence of DNA damage and is associated with decreasing the risk for transmitting mutations to the cell's progeny [150][151][152]. The strictness of the control checkpoint in G1 is relaxed in embryonic cells compared to the requirements of G1/S transition in somatic cells [153].…”
Section: Increasing the Propensity For Differentiation Along Multiplementioning
confidence: 99%
“…The strictness of the control checkpoint in G1 is relaxed in embryonic cells compared to the requirements of G1/S transition in somatic cells [153]. What is more, mammalian embryonic cells are universally sensitive to the presence of DNA damage, but different types of embryonic cells may respond differently to it [152]. In early mouse embryos and in mESC (their in vitro counterparts) the G1 checkpoint is virtually switched off, allowing the cells to proceed with DNA replication even in the presence of DNA damage.…”
Section: Increasing the Propensity For Differentiation Along Multiplementioning
confidence: 99%
“…This is of particular importance in the cells of the early embryo, which are expected to divide quickly to form enough cells so as to lay the progenitors of all cell populations of the adult organism and all DNAmodifying events must be resolved before the cell proceeds to S phase. Embryonic cells are therefore exquisitely sensitive to the presence of DNA damage [81].…”
Section: Signalling Mediated By Erk1/2mentioning
confidence: 99%
“…In any case, there is a risk of genotoxic damage to embryonic cells, and there is not much choice, figuratively speaking, on how to proceed with damage resolution. The G1/S phase provides a major checkpoint in eukaryotic cells (also known as restriction point), and its strictness correlates with the potential risk of letting a cell carrying potentially harmful mutations to produce progeny [81][82][83]. In somatic cells, the presence of the restriction point is obligatory and failure to comply with its requirements usually results in temporary or permanent cell cycle arrest or cell death via the apoptosis pathway.…”
Section: Signalling Mediated By Erk1/2mentioning
confidence: 99%
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