DNA self-assembled FeNxC nanocatalytic network for ultrasensitive electrochemical detection of microRNA

Ning, Yunyun; Zhang, Cuiling; Wang, Chunyan; Zhou, Chuqing; Gong, Nana; Wang, Qiang; Zhu, Ye

doi:10.1016/j.aca.2022.340218

Cited by 3 publications

References 33 publications

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Self-assembled nanomaterials for fabrication of electrochemical biosensors for biomedical applications

Natarajan,

Sivakumar,

Singh

et al. 2024

Electrochemistry

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Self-assembled nanoparticles have proven results in the fabrication of sensors for biomedical applications. In recent years nanoparticles have assembled into clusters by adopting various techniques to develop superstructures. The characteristics of self-assembled nanoparticles are modified by altering the interactions occurring between nano-sized components via external and internal fields. It exhibits easily tuneable and flexible surfaces in multi-dimensional structures. In this chapter, we explored the fabrication and integration of electrochemical sensors with self-assembled nanoparticles. We correlated the properties of self-assembled motifs like size and electrical properties with the sensing performance. Initially, we discussed single-component self-assembled nanomaterials like DNA and peptides and their applications in the biomedical arena. Additionally, peptides will undergo a self-assembly process with the drugs, for targeted tumor therapy. Further self-assembled nature of various transition nano metals/oxides and their role in sensors as electrode support and signal labelling was deliberated. For the fruitful understanding of the title compound the challenges and prospects of electrochemical biosensors in medical applications were also addressed. This chapter will serve as an exhaustive guide to harmonize the vital aspects to formulate stable self-assembled nanostructures for biomedical applications.

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Self-assembled nanomaterials for fabrication of electrochemical biosensors for biomedical applications

Natarajan,

Sivakumar,

Singh

et al. 2024

Electrochemistry

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State-of-the-Art Fluorescent Probes: Duplex-Specific Nuclease-Based Strategies for Early Disease Diagnostics

et al. 2022

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Precision healthcare aims to improve patient health by integrating prevention measures with early disease detection for prompt treatments. For the delivery of preventive healthcare, cutting-edge diagnostics that enable early disease detection must be clinically adopted. Duplex-specific nuclease (DSN) is a useful tool for bioanalysis since it can precisely digest DNA contained in duplexes. DSN is commonly used in biomedical and life science applications, including the construction of cDNA libraries, detection of microRNA, and single-nucleotide polymorphism (SNP) recognition. Herein, following the comprehensive introduction to the field, we highlight the clinical applicability, multi-analyte miRNA, and SNP clinical assays for disease diagnosis through large-cohort studies using DSN-based fluorescent methods. In fluorescent platforms, the signal is produced based on the probe (dyes, TaqMan, or molecular beacon) properties in proportion to the target concentration. We outline the reported fluorescent biosensors for SNP detection in the next section. This review aims to capture current knowledge of the overlapping miRNAs and SNPs’ detection that have been widely associated with the pathophysiology of cancer, cardiovascular, neural, and viral diseases. We further highlight the proficiency of DSN-based approaches in complex biological matrices or those constructed on novel nano-architectures. The outlooks on the progress in this field are discussed.

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Hybridization Chain Reaction-Based Electrochemical Biosensors by Integrating the Advantages of Homogeneous Reaction and Heterogeneous Detection

et al. 2023

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The conventional hybridization chain reaction (HCR)-based electrochemical biosensors usually require the immobilization of probes on the electrode surface. This will limit the applications of biosensors due to the shortcomings of complex immobilization processes and low HCR efficiency. In this work, we proposed astrategy for the design of HCR-based electrochemical biosensors by integrating the advantages of homogeneous reaction and heterogeneous detection. Specifically, the targets triggered the autonomous cross-opening and hybridization oftwobiotin-labeled hairpin probes to form long-nicked dsDNA polymers. The HCR products with many biotin tags were then captured by a streptavidin-covered electrode, thus allowing for the attachment of streptavidin-conjugated signal reporters through streptavidin–biotin interactions. By employing DNA and microRNA-21 as the model targets and glucose oxidase as the signal reporter, the analytical performances of the HCR-based electrochemical biosensors were investigated. The detection limits of this method were found to be 0.6 fM and 1 fM for DNA and microRNA-21, respectively. The proposed strategy exhibited good reliability for target analysis in serum and cellular lysates. The strategy can be used to develop various HCR-based biosensors for a wide range of applications because sequence-specific oligonucleotides exhibit high binding affinity to a series of targets. In light of the high stability and commercial availability of streptavidin-modified materials, the strategy can be used for the design of different biosensors by changing the signal reporter and/or the sequence of hairpin probes.

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DNA self-assembled FeNxC nanocatalytic network for ultrasensitive electrochemical detection of microRNA

Cited by 3 publications

References 33 publications

Self-assembled nanomaterials for fabrication of electrochemical biosensors for biomedical applications

Self-assembled nanomaterials for fabrication of electrochemical biosensors for biomedical applications

State-of-the-Art Fluorescent Probes: Duplex-Specific Nuclease-Based Strategies for Early Disease Diagnostics

Hybridization Chain Reaction-Based Electrochemical Biosensors by Integrating the Advantages of Homogeneous Reaction and Heterogeneous Detection

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