DNA-Specific DAPI Staining of the Pyrenoid Matrix During its Fission in Dunaliella salina (Dunal) Teodoresco (Chlorophyta)

Oleksienko, Anna A; Kot, Yurii; Komaristaya, V. P.

doi:10.1007/s00284-020-02159-0

Cited by 4 publications

(3 citation statements)

References 57 publications

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“…This phenomenon was potentially associated with the concurrent division of chloroplasts and equitable distribution of the pyrenoid during cellular division. 34 After cell division, the small daughter cells exhibited a relatively higher intracellular Cu(II) fluorescence intensity. Intriguingly, it appeared that the labile Cu(II) pool predominantly formed and accumulated during the SM phase, as evidenced by the constancy of the total unicellular Cu(II) fluorescence intensity during the G phase and its significant elevation during the SM phase (Figure 1D).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…These and other textural parameter trends underscore the intricate arrangement of labile Cu(II) throughout the cell cycle. This phenomenon was potentially associated with the concurrent division of chloroplasts and equitable distribution of the pyrenoid during cellular division . After cell division, the small daughter cells exhibited a relatively higher intracellular Cu(II) fluorescence intensity.…”

Section: Results and Discussionmentioning

confidence: 99%

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Dynamic Regulation of Intracellular Labile Cu(I)/Cu(II) Cycle in Microalgae Chlamydomonas reinhardtii: Disrupting the Balance by Cu Stress

Deng,

Wang

2024

Environ. Sci. Technol.

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The labile metal pool involved in intracellular trafficking and homeostasis is the portion susceptible to environmental stress. Herein, we visualized the different intracellular distributions of labile Cu(I) and Cu(II) pools in the alga Chlamydomonas reinhardtii. We first demonstrated that labile Cu(I) predominantly accumulated in the granules within the cytoplasmic matrix, whereas the labile Cu(II) pool primarily localized in the pyrenoid and chloroplast. The cell cycle played an integral role in balancing the labile Cu(I)/Cu(II) pools. Specifically, the labile Cu(II) pool primarily accumulated during the SM phase following cell division, while the labile Cu(I) pool dynamically changed during the G phase as cell size increased. Notably, the labile Cu(II) pool in algae at the SM stage exhibited heightened sensitivity to environmental Cu stress. Exogenous Cu stress disrupted the intracellular labile Cu(I)/Cu(II) cycle and balance, causing a shift toward the labile Cu(II) pool. Our proteomic analysis further identified a putative cupric reductase, potentially capable of reducing Cu(II) to Cu(I), and four putative multicopper oxidases, potentially capable of oxidizing Cu(I) to Cu(II), which may be involved in the conversion between the labile Cu(I) pool and labile Cu(II) pool. Our study elucidated a dynamic cycle of the intracellular labile Cu(I)/Cu(II) pools, which were accessible and responsive to environmental changes.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%

Dynamic Regulation of Intracellular Labile Cu(I)/Cu(II) Cycle in Microalgae Chlamydomonas reinhardtii: Disrupting the Balance by Cu Stress

Deng,

Wang

2024

Environ. Sci. Technol.

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“…DAPI is a uorescent stain that can stain living cells and emit blue-violet uorescence under the excitation of laser at the appropriate wavelength. 36,37 It is a uorescent stain that can stain living cells. PI is a nuclear staining reagent that can stain DNA and is oen used for apoptosis detection.…”

Section: Antibacterial Properties For Escherichia Colimentioning

confidence: 99%

Enhanced bacterial disinfection by CuI–BiOI/rGO hydrogel under visible light irradiation

Wang

Yang

et al. 2021

RSC Adv.

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Discovery of membrane-targeting amphiphilic honokiol derivatives containing an oxazolethione moiety to combat methicillin-resistant Staphylococcus aureus (MRSA) infections

Yang,

Cui,

et al. 2024

European Journal of Medicinal Chemistry

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DNA-Specific DAPI Staining of the Pyrenoid Matrix During its Fission in Dunaliella salina (Dunal) Teodoresco (Chlorophyta)

Cited by 4 publications

References 57 publications

Dynamic Regulation of Intracellular Labile Cu(I)/Cu(II) Cycle in Microalgae Chlamydomonas reinhardtii: Disrupting the Balance by Cu Stress

Dynamic Regulation of Intracellular Labile Cu(I)/Cu(II) Cycle in Microalgae Chlamydomonas reinhardtii: Disrupting the Balance by Cu Stress

Enhanced bacterial disinfection by CuI–BiOI/rGO hydrogel under visible light irradiation

Discovery of membrane-targeting amphiphilic honokiol derivatives containing an oxazolethione moiety to combat methicillin-resistant Staphylococcus aureus (MRSA) infections

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