DNA-surfactant complexes in organic media

Sergeyev, Vladimir G.; Pyshkina, O. A.; Gallyamov, Marat O.; Yaminsky, I.; Зезин, А. Б.; Kabanov, V.A.

doi:10.1007/bf01189520

Cited by 15 publications

(20 citation statements)

References 10 publications

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“…The total chain length, however, is considered to be much larger than l p , and the entire polymer cannot be regarded, therefore, as a single rigid rod. This case corresponds, in particular, to experiments on long DNA molecules [3][4][5][6][7][8][9][10], whose persistence length is typically very large (of order 50 nm), but much smaller than the total chain length (which is usually larger than a micron) [27]. We argue that such an intermediate system may, in certain cases, be governed by different physics.…”

Section: Introductionmentioning

confidence: 83%

“…In addition, there has been growing interest in the interactions between DNA macromolecules and surfactants, lipids and short polyamines [3][4][5][6][7][8][9][10]. These interactions are relevant to various biochemical applications such as DNA extraction and purification [8][9][10] and genetic delivery systems [11]. Association of folded proteins (e.g., RecA) with DNA plays a key role in genetic regulatory mechanisms.…”

Section: Introductionmentioning

confidence: 99%

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Binding of molecules to DNA and other semiflexible polymers

Diamant

Andelman

2000

Phys. Rev. E

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A theory is presented for the binding of small molecules such as surfactants to semiflexible polymers. The persistence length is assumed to be large compared to the monomer size but much smaller than the total chain length. Such polymers (e.g., DNA) represent an intermediate case between flexible polymers and stiff, rod-like ones, whose association with small molecules was previously studied. The chains are not flexible enough to actively participate in the self-assembly, yet their fluctuations induce long-range attractive interactions between bound molecules. In cases where the binding significantly affects the local chain stiffness, those interactions lead to a very sharp, cooperative association. This scenario is of relevance to the association of DNA with surfactants and compact proteins such as RecA. External tension exerted on the chain is found to significantly modify the binding by suppressing the fluctuation-induced interaction.61.25.Hq,87.15.Nn,87.14.Gg

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Section: Introductionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Binding of molecules to DNA and other semiflexible polymers

Diamant

Andelman

2000

Phys. Rev. E

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“…[1] Thus, SFM has been successfully employed to study DNA, [2] RNA, [3] and their complexes with proteins, [4] surfactants [5] and other compounds. The ex situ visualization of conformational changes of DNA molecules has been carried out for the effect imposed by interaction with oppositely charged silanes, [6] with polymer nanoparticles, [7] surfactants, [8] Mg 2 + ions (in ethanol), [9] spermidine, [10,11] polylysine, [12,13] poly(ethylene glycol)-poly(amidoamine) copolymer, [14] lipospermine and polyethylenimine.…”

Section: Introductionmentioning

confidence: 99%

Reversible Collapse of Brushlike Macromolecules in Ethanol and Water Vapours as Revealed by Real‐Time Scanning Force Microscopy

Gallyamov

Tartsch

Khokhlov

et al. 2004

Chemistry A European J

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Environment-controlled scanning force microscopy allowed us to study adsorption and desorption of single poly(methacrylate)-graft-poly(n-butyl acrylate) brush molecules on mica in real time. The molecules transform reversibly from a two-dimensional, extended wormlike state to a compact globular state. The dynamics of the conformational transition was sufficiently slow in order to allow its observation by scanning force microscope in real time. The reversible transformation is effected by coadsorption of water or ethanol, the latter introduces the collapse. Adsorbing ethanol and water from the vapour atmosphere results in a change of the surface properties of mica, either favouring adsorption or desorption of the graft polymer. When the extended, tightly adsorbed poly(n-butyl acrylate) brush molecules are exposed to ethanol vapour, the macromolecules swell and contract to form compact globules. Exchanging the ethanol vapour to a humid atmosphere caused the molecules to extend again to a wormlike two-dimensional conformation. Coexistence of collapsed and extended strands within the same molecule indicates a single-molecule first-order transition in agreement with observations on Langmuir films previously reported.

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“…15,16 However, the resolution limit of wavelength of fluorescence light fails to present a more accurate analysis of the structure of DNAbased complex. 17 In view of that, we use atomic force microscopy to analyze the morphology of DNA/CS-12 complex. Figure 2 displays the AFM image of DNA/CS-12 complex.…”

Section: Resultsmentioning

confidence: 99%

Formation of a DNA/N‐dodecylated chitosan complex and salt‐induced gene delivery

Liu

Yao

Liu

2001

J of Applied Polymer Sci

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An N-dodecylated chitosan (CS-12) was synthesized from dodecyl bromide and chitosan and was assembled with DNA to form a polyelectrolyte complex (DNA/ CS-12 PEC). UV was used to examine the thermal stability of DNA embedded in PEC. The results indicate that the incorporation of dodecylated chitosan can enhance the thermal stability of DNA. The analysis of AFM image shows that PEC develops a globule-like structure composed of 40 -115 DNA molecules. Dissociation of PEC was investigated by the addition of low molecular weight electrolytes. The added small molecular salts dissociate the PEC, inducing DNA to release. The ability of Mg 2ϩ to dissociate PEC is greater compared to that of Na ϩ and K ϩ . From AFM images, it can be visualized that DNA remains intact and undamaged due to the protection from DNase offered by alkylated chitosan.

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DNA-surfactant complexes in organic media

Cited by 15 publications

References 10 publications

Binding of molecules to DNA and other semiflexible polymers

Binding of molecules to DNA and other semiflexible polymers

Reversible Collapse of Brushlike Macromolecules in Ethanol and Water Vapours as Revealed by Real‐Time Scanning Force Microscopy

Formation of a DNA/N‐dodecylated chitosan complex and salt‐induced gene delivery

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