DNA transfer to worn upper garments during different activities and contacts: An inter-laboratory study

Szkuta, Bianca; Ansell, Ricky; Boiso, Lina; Connolly, E. Sander; Kloosterman, A.; Kokshoorn, Bas; McKenna, Louise; Steensma, Kristy; Oorschot, Roland A.H. van

doi:10.1016/j.fsigen.2020.102268

Cited by 14 publications

(16 citation statements)

References 20 publications

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“…It has been shown that variations in DNA processing methods, especially those prior to the introduction of more sensitive methods (in 2012-2017, depending on jurisdiction), can also influence the quantity of DNA recovered and composition of profiles generated (e.g., as illustrated by environmental DNA monitoring programs [Ballantyne, Poy, & Van Oorschot, 2013] and interlaboratory studies on cable ties [Steensma et al, 2017] and worn clothing [Szkuta et al, 2019[Szkuta et al, , 2020). Few studies have investigated the impact of interlaboratory differences in the methods applied on DNA profiling outcomes, and fewer still have assessed the consequences of downstream use of such data for evaluations given activity level propositions.…”

Section: Types Of Data Available For Usementioning

confidence: 99%

“…Studies have been conducted on the presence of background DNA from unknown individuals on surfaces and items in various public and private spaces (e.g., van den Berge, Ozcanhan, et al, 2016). A small number of recent studies revealed that in private spaces, many of these unknowns appear to be close associates of the owner/user of the item, such as their partners and other house inhabitants, or work colleagues (Boyko et al, 2020;Goray et al, 2020;Reither et al, 2019;Szkuta et al, 2019Szkuta et al, , 2020Taylor, Abarno, Rowe, & Rask-Nielsen, 2016). In addition to highlighting the frequency and relative contributions of the known and unknown associates of the person, item, and/or environment of interest in which various individuals are observed under different conditions, these studies have highlighted the importance of obtaining elimination reference samples to assist in DNA profile interpretation.…”

Section: Supplementary Knowledge From Caseworkmentioning

confidence: 99%

“…Subsequent research studies have also highlighted limitations regarding the use of total DNA quantities to inform evaluations given activity level propositions. First, high intraperson and interperson variability in DNA quantities, for example, recovered from knives (Butcher et al, 2019; G. E. Meakin, Butcher, van Oorschot, & Morgan, 2017; Samie et al, 2016), guns (Gosch & Courts, 2019), and clothing (Szkuta et al, 2019, 2020) used in different scenarios, limits the reproducibility of DNA quantities for similar activities. Second, such variation, specifically differences in the shedder status of individuals, has been shown to impact the probabilities of DNA transfer to worn T‐shirts in a simulated attack scenario (Fonneløp et al, 2017).…”

Section: The Path Forwardmentioning

confidence: 99%

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Evaluating forensic DNA evidence: Connecting the dots

Meakin

Kokshoorn

Oorschot

et al. 2020

WIREs Forensic Science

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Technological developments within the field of forensic genetics have enhanced the sensitivity and specificity of DNA testing tremendously and have broadened the applications of biological evidence in criminal investigations.Evaluation and communication of the evidential findings within criminal cases have not maintained the same pace, which has largely stemmed from a failure to adopt a standardized approach within and across the various fields of forensic science. Within forensic biology, this has led to unjustified opinions on the weight of the evidence and occurrences of the association fallacy, when the weight of evidence given propositions at one level of the hierarchy of propositions is inappropriately transposed to a higher level. We further define the association fallacy to include the terms, "source level fallacy" and "activity level fallacy," to enable the forensic science community to better identify and address issues in biological evidence evaluation. It is important to understand these concepts and their causes, and in doing so, identify potential avenues to avoid these fallacies in forensic science casework. These avenues include training and education of forensic and legal professionals, as well as research into transfer, persistence, prevalence and recovery (TPPR) of DNA and biological evidence in general.

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Section: Types Of Data Available For Usementioning

confidence: 99%

Section: Supplementary Knowledge From Caseworkmentioning

confidence: 99%

Section: The Path Forwardmentioning

confidence: 99%

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Evaluating forensic DNA evidence: Connecting the dots

Meakin

Kokshoorn

Oorschot

et al. 2020

WIREs Forensic Science

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“…Many terrorism and natural disasters have caused ISSN : 1411 3724 Eksakta : Berkala Ilmiah Bidang MIPA many fatalities, some of which can no longer be recognized or identified due to damage to some or all parts of the victim's body. To be able to identify victims, genetic information is needed (DNA (deoxyrebous nucleid acid) from the victim [9][10][11][12][13][14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

“…DNA is a biological fingerprint of an individual obtained from the genetic inheritance of parents [9][10][11][12][13][14][15][16][17][18][19][20] (21,22,24), DNA profile identification is carried out on DNA evidence contained in human body tissues such as hair, teeth, blood, urine, saliva, muscles and sperm (23). The DNA profile maps the genetic makeup of individuals consisting of 16 loci, namely CSF1PO, D13S317, D16S539, D18S51, D19S433, D21S11, D2S1338, D3S1358, D5S818, D7S720, D8S1179, FGA, TH01, T19X, D21S11, D2S1338, D3S1358, D5S818, D7S720, D8S1179, FGA, TH01, T19X, and D21S11, D2S1338, D3S1358, D5S818, D7S720, D8S1179, FGA, TH01, TPOX, and VWA.…”

Section: Introductionmentioning

confidence: 99%

The Similarity Measurement of Human DNA Profile Using Fuzzy Similarity

Dewi

Soedarsono

2020

Eksakta

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This research investigated the similarity of human DNA profile using fuzzy similarity measure. The similarity measurement of DNA profile had been done by measuring the similarity between query’s DNA profile and its biological family such as father, mother, brother, sister, grandmother and grandfather. The similarity measurement had been done to the short tandem repeat (STR) alleles in sixteen loci. The result of the experiment showed that each simulation gave matching result. This research is useful for Indonesian National Police (POLRI) in identifying process of disaster victim, terrorism victim and other criminal conduct.

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Comparison of genotyping and weight of evidence results when applying different genotyping strategies on samples from a DNA transfer experiment

et al. 2022

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In this study we assessed to what extent data on the subject of TPPR (transfer, persistence, prevalence, recovery) that are obtained through an older STR typing kit can be used in an activity-level evaluation for a case profiled with a more modern STR kit. Newer kits are generally more sensitive and consist of more loci which could increase the evidential value at the source-level. On the other hand, the increased genotyping information may invoke a higher number of contributors in weight of evidence calculations, which could affect the evidential values as well. An activity scenario well explored in earlier studies [1,2] was redone using volunteers with known DNA profiles. DNA extracts were analyzed with three different approaches, namely using the optimal DNA input for 1) an older and 2) a newer STR typing system, and 3) using a standard, volume-based input combined with replicate PCR analysis with only the newer STR kit. The genotyping results were analyzed for various aspects such as percentage detected alleles and relative peak height contribution for background and the contributors known to be involved in the activity. Next, source-level LRs were calculated and the same trends were observed with regards to inclusionary and exclusionary LRs for persons who had or had not been into direct contact with the sampled areas. We subsequently assessed the impact on the outcome of activity-level evaluation in an exemplary case by applying the assigned probabilities to a Bayesian network. We infer that data of different STR kits can be combined in activity-level evaluations.

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DNA transfer to worn upper garments during different activities and contacts: An inter-laboratory study

Cited by 14 publications

References 20 publications

Evaluating forensic DNA evidence: Connecting the dots

Evaluating forensic DNA evidence: Connecting the dots

The Similarity Measurement of Human DNA Profile Using Fuzzy Similarity

Comparison of genotyping and weight of evidence results when applying different genotyping strategies on samples from a DNA transfer experiment

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