DNA vaccination of neonate piglets in the face of maternal immunity induces humoral memory and protection against a virulent pseudorabies virus challenge

Fischer, Laurent; Barzu, Simona; Andréoni, C.; Buisson, Nathalie; Brun, A; Audonnet, Jean Christophe

doi:10.1016/s0264-410x(02)00736-3

Cited by 44 publications

(19 citation statements)

References 26 publications

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“…Furthermore, DNA-based immunization enables the use of a very pure immunogen, since plasmid preparations can readily be purified from protein contaminants. Finally, DNA immunization has been shown to be capable of inducing immunity irrespective of the presence of maternally derived antibodies (4,10). The strong immunogenicity of ORF2/IL18-encoding plasmid in piglets suggests that the pBudCE4.1-ORF2/IL18 plasmid may be useful in the control of PCV2 infections on both an individual and a population basis, even in the presence of maternally derived immunity.…”

Section: Discussionmentioning

confidence: 99%

Immune Responses of Piglets Immunized by a Recombinant Plasmid Containing Porcine Circovirus Type 2 and Porcine Interleukin-18 Genes

Chen

Wang

et al. 2014

Viral Immunology

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In this study, two recombinant plasmids containing the ORF2 gene of porcine circovirus type 2 (PCV2) with or without porcine interleukin-18 (IL-18) were constructed and evaluated for their ability to protect piglets against PCV2 challenge. Transient expression of the plasmids in PK-15 cells could be detected using Western blot. Piglets were given two intramuscular immunizations 3 weeks apart and were challenged with a virulent Wuzhi strain of PCV2 at 42 days after the initial immunization. All animals vaccinated with pBudCE4

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Section: Discussionmentioning

confidence: 99%

Immune Responses of Piglets Immunized by a Recombinant Plasmid Containing Porcine Circovirus Type 2 and Porcine Interleukin-18 Genes

Chen

Wang

et al. 2014

Viral Immunology

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“…The most compelling feature of DNA vaccination is related to safety, because they can be manufactured in the absence of substances of animal origin and can be highly purified. Further advantages are related to the de novo synthesis of antigens structurally identical to those produced during an active infection resulting in the stimulation of both humoral and cellular immune responses [83] and to their ability to overcome maternal derived immunity in neonates or in very young animals [28]. However, a disadvantage of DNA vaccines is that they trigger relatively weak serological responses against certain antigens when compared to conventional vaccines.…”

Section: Dna Vaccinesmentioning

confidence: 99%

Equine viral vaccines: the past, present and future

2004

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-The increasing international movement of horses combined with the relaxation of veterinary regulations has resulted in an increased incidence of equine infectious diseases. Vaccination, along with management measures, has become the primary method for the effective control of these diseases. Traditionally modified live and inactivated vaccines have been used and these vaccines have proven to be very successful in preventing disease. However, there are a number of equine infectious diseases for which conventional technology has shown its limitations. The advent of recombinant technology has stimulated the development of second generation vaccines, including gene deleted mutants, live vectored vaccines and DNA vaccines. These vaccines have in common that protective antigens are endogenously processed and presented along the molecules of the MHC I and MHC II complex, resulting in the stimulation of both humoral and cell-mediated immune responses similar to natural infection. The present paper provides a review of the vaccines being employed today against the most important equine viral diseases followed by a summary of new developments that are expected to bring improved vaccines to the market in the foreseeable future.

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“…Immunization based on DNA vaccines has more advantages than the conventional vaccination methods as it eliminates the need to perform conventional antigen preparation, which is rather laborious. It enables the use of a more pure immunogen, since plasmidial preparations could be easily purified, besides being capable of inducing immunity even in the presence of maternal antibodies (Hassett et al 1997, Fischer et al 2003. Differently from inactivated or subunit vaccines, DNA vaccines may result in an antigenic presentation via Class I and Class II MHC molecules, which mimics the resulting response to natural infection, inducing CD4 + and CD8 + T lymphocytes and antibody production.…”

Section: Discussionmentioning

confidence: 99%

Development and evaluation of a recombinant DNA vaccine candidate expressing porcine circovirus 2 structural protein

Júnior¹,

Castro²,

Chiarelli-Neto³

et al. 2009

Pesq. Vet. Bras.

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RESUMO.-[Desenvolvimento e avaliação de um candidato à vacina de DNA recombinante expressando a proteína estrutural do circovírus suíno 2.] O circovírus suíno 2 (PCV2) é geralmente associado à síndrome da circovirose suína, que é considerada uma importante doença de suínos e possui um sério impacto econômico na suinocultura mundial. Este trabalho descreve a construção de um plasmídeo recombinante que expressa a proteína estrutural do PCV2 e a avaliação das respostas imune humoral e celular por meio de vacinação em camundongos BALB/c. O candidato vacinal foi submetido a aná-lises in vivo, determinando a capacidade de induzir resposta imune específica em camundongos. O DNA de um isolado brasileiro de PCV2 foi extraído e o gene que codifica para a proteína do capsídeo foi amplificado por PCR e inserido num plasmídeo de expressão.

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DNA vaccination of neonate piglets in the face of maternal immunity induces humoral memory and protection against a virulent pseudorabies virus challenge

Cited by 44 publications

References 26 publications

Immune Responses of Piglets Immunized by a Recombinant Plasmid Containing Porcine Circovirus Type 2 and Porcine Interleukin-18 Genes

Immune Responses of Piglets Immunized by a Recombinant Plasmid Containing Porcine Circovirus Type 2 and Porcine Interleukin-18 Genes

Equine viral vaccines: the past, present and future

Development and evaluation of a recombinant DNA vaccine candidate expressing porcine circovirus 2 structural protein

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